Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQ393_RS10765 Genome accession   NZ_CP058627
Coordinates   2287395..2289101 (+) Length   568 a.a.
NCBI ID   WP_179355186.1    Uniprot ID   A0A7H9BK23
Organism   Chitinibacter bivalviorum strain 2T18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2282395..2294101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ393_RS10735 (HQ393_10735) rplS 2282808..2283188 (-) 381 WP_179355182.1 50S ribosomal protein L19 -
  HQ393_RS10740 (HQ393_10740) trmD 2283229..2283990 (-) 762 WP_179358479.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  HQ393_RS10745 (HQ393_10745) rimM 2284040..2284585 (-) 546 WP_308419946.1 ribosome maturation factor RimM -
  HQ393_RS10750 (HQ393_10750) rpsP 2284596..2284844 (-) 249 WP_179355183.1 30S ribosomal protein S16 -
  HQ393_RS10755 (HQ393_10755) ffh 2284944..2286287 (-) 1344 WP_179355184.1 signal recognition particle protein -
  HQ393_RS10760 (HQ393_10760) - 2286369..2287175 (+) 807 WP_179355185.1 inner membrane protein YpjD -
  HQ393_RS10765 (HQ393_10765) pilB 2287395..2289101 (+) 1707 WP_179355186.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HQ393_RS10770 (HQ393_10770) pilC 2289115..2290341 (+) 1227 WP_179355187.1 type II secretion system F family protein Machinery gene
  HQ393_RS10775 (HQ393_10775) pilD 2290346..2291203 (+) 858 WP_179355188.1 A24 family peptidase Machinery gene
  HQ393_RS10780 (HQ393_10780) coaE 2291203..2291808 (+) 606 WP_179355189.1 dephospho-CoA kinase -
  HQ393_RS10785 (HQ393_10785) zapD 2291871..2292629 (+) 759 WP_179355190.1 cell division protein ZapD -
  HQ393_RS10790 (HQ393_10790) - 2292629..2292820 (+) 192 WP_179355191.1 DNA gyrase inhibitor YacG -
  HQ393_RS10795 (HQ393_10795) - 2292918..2293670 (+) 753 WP_179355192.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62474.92 Da        Isoelectric Point: 5.3024

>NTDB_id=464678 HQ393_RS10765 WP_179355186.1 2287395..2289101(+) (pilB) [Chitinibacter bivalviorum strain 2T18]
MSTPQISGLARLLVQHGRLSDADAEALQASANTNKTTFIEQLTQSKKLSARDVAEFSSQTFGYPLLDIDRIDSSYLPQGV
IDNNVMRAQRIVPLFKRGTKLFIGISDITNLEAVEKVRFQTNAQVEPIIVEDNKLIVLINRTIDATGANLKEMDLEDVEL
ALDGGDPEPTAAETQMGQDIDDAPIVKYLQKILMDAINGGASDIHFEPYEKFYRIRFRIDGVLREIAQPPLAIKEKISSR
IKVISKLDISEKRIPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKAIILEAIQRPY
GMVLVTGPTGSGKTVSLYTCLNLLNKPDINIATAEDPCEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKKHIDLPNQALIDAGFTQ
EDLDGTWQPYGPVGCEICKDTGYKGRVGIYQVMPITDEINRLIMNHGNAIDIADQARKEGVLSLREAGLRKVKQGLTSLE
EVMAVTNV

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=464678 HQ393_RS10765 WP_179355186.1 2287395..2289101(+) (pilB) [Chitinibacter bivalviorum strain 2T18]
ATGAGCACCCCGCAAATTTCTGGTCTCGCACGCTTACTCGTGCAGCATGGCCGACTGAGCGATGCAGATGCCGAAGCACT
GCAAGCGAGCGCCAACACAAATAAAACCACTTTTATTGAGCAATTAACTCAAAGCAAAAAGCTATCGGCACGGGATGTCG
CAGAGTTCTCATCTCAAACGTTTGGCTACCCTCTGCTCGACATCGATCGAATTGACTCCAGCTATTTGCCGCAAGGTGTA
ATCGACAATAACGTTATGCGCGCCCAGCGCATTGTTCCGCTATTCAAACGCGGTACAAAACTTTTCATCGGCATTTCAGA
TATCACCAATCTGGAAGCAGTTGAAAAAGTTCGCTTCCAAACCAACGCGCAAGTCGAACCGATTATCGTTGAAGACAACA
AGCTCATCGTTCTCATCAATAGAACAATTGATGCCACAGGTGCCAACCTGAAAGAAATGGACCTCGAAGATGTGGAGCTT
GCACTCGACGGCGGCGACCCCGAACCAACAGCGGCTGAAACTCAAATGGGGCAAGATATTGACGATGCCCCCATTGTTAA
ATATCTGCAAAAAATCTTGATGGATGCCATCAATGGCGGCGCATCCGACATTCACTTTGAGCCGTACGAAAAATTCTACC
GGATTCGTTTTCGCATCGACGGGGTACTCCGCGAAATCGCCCAGCCGCCATTAGCAATCAAAGAGAAAATATCATCCCGC
ATCAAGGTCATTTCGAAGCTCGACATTTCAGAAAAGCGCATTCCACAAGATGGACGCATGAAATTAGTCCTGTCTAAATC
GCGAGCTATTGATTTCCGGGTCTCCACCCTGCCGACCATGCATGGTGAAAAAATTGTAATGCGGATTTTGGATCCTTCAT
CCGCAACGCTCGGCATTGATGCTCTCGGCTACGATCCAGATCAAAAAGCCATTATCCTTGAAGCAATTCAGCGCCCGTAT
GGCATGGTCTTAGTGACAGGCCCGACCGGCTCAGGCAAAACCGTATCGCTTTACACATGCCTTAATTTACTCAATAAGCC
AGATATCAATATCGCAACGGCGGAAGACCCATGCGAGATTAACTTGCCGGGCATCAACCAGGTCAATGTAAATGACAAAG
CGGGGCTAACGTTCTCTGCCGCACTCAAGGCCTTCCTGCGTCAAGACCCCGACATCATTATGGTCGGCGAGATTCGCGAC
CTAGAAACCGCCGATATCGCAATTAAAGCTGCGCAAACGGGCCACATGGTGTTTTCAACACTCCACACCAATGATGCCCC
AACCACATTAACGCGAATGCTCAATATGGGCATCCAGCCGTTCAATATTGCTTCATCGGTCATTCTAATTACCGCTCAGC
GCCTAGCTCGCCGTCTATGCAGTTGCAAAAAACACATTGACCTTCCAAATCAGGCCTTAATCGACGCAGGCTTTACCCAA
GAAGACTTGGATGGCACATGGCAGCCGTACGGCCCCGTCGGCTGCGAAATATGCAAAGACACTGGCTACAAAGGTCGTGT
TGGTATTTACCAAGTTATGCCGATTACAGATGAAATCAACCGACTAATCATGAACCATGGCAATGCCATTGATATTGCTG
ATCAGGCTCGCAAAGAAGGCGTACTAAGCCTTCGTGAAGCAGGACTTCGAAAAGTTAAACAAGGCCTCACCTCACTTGAA
GAAGTGATGGCCGTTACAAATGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-144)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H9BK23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.185

100

0.553
  pilF Neisseria gonorrhoeae MS11

54.225

100

0.542
  pilB Legionella pneumophila strain ERS1305867

52.632

100

0.528
  pilB Vibrio cholerae strain A1552

46.018

99.472

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.293

99.12

0.449
  pilB Vibrio campbellii strain DS40M4

44.504

99.296

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.769

100

0.41
  pilF Thermus thermophilus HB27

37.786

100

0.379