Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HW090_RS06945 Genome accession   NZ_CP058349
Coordinates   1580660..1582363 (-) Length   567 a.a.
NCBI ID   WP_256930760.1    Uniprot ID   -
Organism   Pseudomonas sp. ABC1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1575660..1587363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW090_RS06910 (HW090_06910) rpsI 1575793..1576185 (-) 393 WP_179112822.1 30S ribosomal protein S9 -
  HW090_RS06915 (HW090_06915) rplM 1576200..1576628 (-) 429 WP_179112823.1 50S ribosomal protein L13 -
  HW090_RS06920 (HW090_06920) - 1576975..1577664 (+) 690 WP_179112824.1 energy-coupling factor ABC transporter permease -
  HW090_RS06925 (HW090_06925) yacG 1577697..1577888 (-) 192 WP_179112825.1 DNA gyrase inhibitor YacG -
  HW090_RS06930 (HW090_06930) coaE 1577885..1578493 (-) 609 WP_373416369.1 dephospho-CoA kinase -
  HW090_RS06935 (HW090_06935) pilD 1578569..1579438 (-) 870 WP_179112827.1 A24 family peptidase Machinery gene
  HW090_RS06940 (HW090_06940) pilC 1579442..1580647 (-) 1206 WP_256930759.1 type II secretion system F family protein Machinery gene
  HW090_RS06945 (HW090_06945) pilB 1580660..1582363 (-) 1704 WP_256930760.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HW090_RS06950 (HW090_06950) pilA 1582664..1582834 (+) 171 WP_179112828.1 type IV pilin protein Machinery gene
  HW090_RS06955 (HW090_06955) - 1582847..1583317 (+) 471 WP_179112829.1 hypothetical protein -
  HW090_RS06960 (HW090_06960) - 1583671..1584240 (-) 570 WP_179112830.1 YiiX/YebB-like N1pC/P60 family cysteine hydrolase -
  HW090_RS06965 (HW090_06965) - 1584240..1584644 (-) 405 WP_179112831.1 DUF4878 domain-containing protein -
  HW090_RS06970 (HW090_06970) - 1584731..1585072 (+) 342 WP_179112832.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HW090_RS06975 (HW090_06975) - 1585133..1585768 (-) 636 WP_179112833.1 pseudouridine synthase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62040.28 Da        Isoelectric Point: 5.8522

>NTDB_id=463993 HW090_RS06945 WP_256930760.1 1580660..1582363(-) (pilB) [Pseudomonas sp. ABC1]
MDVFRHKDESVEPGIALTGLACELIESGLLDEQQIRQAQKEADRERISLVAWLARHRLVDSQRLMLLSGRAFGMPCFDLD
LFDMGHAPAGLVSEKMARTHRVLPLQLQGNRLRVAVSDPANHQAISDIQFCTGLCVETVLVDDELLSRVLDRFYAPAGAD
LGAEPDSLAISDDAREIPPDGSEDAPAVRLVRDVLLRAVKEGASDLHFEPHEKYYRIRCRTDGLLYELARPPMSLASRIS
TRLKVMAGMDIAERRRPQDGRARLDAADGRPVDMRVSTLPALWGERIVIRILDASLSCLGVDELGLEAEQQRLYLSALRQ
SRGLILVTGPTGSGKTRTLYSGLATLNTGSLNISTVEDPVEISLDGTCQVNINPRQGLGFAQVSRALLRQDPDVIMVGEI
RDPETAEVAIKAAQTGHLVLSTLHTNSAAQTLERLRNLGIPAFNIAAAVSLIVAQRLVRRLCTCKREISLPQEVLLQEGF
PPESMGCFTLFEGVGCSECRDGYRGRTGIHEVVRITPELQQLIMRGGNSIEMAALATAEGFHSLRVAGLLKVMQGVTSLS
EVNRVMG

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=463993 HW090_RS06945 WP_256930760.1 1580660..1582363(-) (pilB) [Pseudomonas sp. ABC1]
ATGGACGTCTTTCGACATAAAGACGAGTCAGTGGAGCCGGGTATTGCTCTGACCGGCTTGGCATGCGAGTTGATCGAATC
AGGGCTGCTGGATGAGCAGCAGATACGGCAAGCTCAGAAAGAGGCGGATCGTGAGCGAATATCACTGGTTGCCTGGCTTG
CACGGCACCGTCTGGTCGATAGCCAGCGGTTGATGCTGTTGTCCGGCAGGGCTTTCGGAATGCCTTGCTTCGATCTCGAC
CTGTTCGACATGGGGCACGCTCCCGCTGGATTGGTCAGCGAGAAAATGGCCAGGACGCACCGCGTGCTACCACTGCAGTT
GCAGGGTAATCGCCTGCGTGTGGCCGTCTCCGACCCTGCAAACCACCAGGCTATCAGCGACATTCAGTTCTGTACGGGGT
TGTGCGTGGAGACCGTGCTGGTCGACGACGAACTGCTCAGTAGGGTCTTGGACCGGTTTTATGCTCCCGCCGGGGCTGAC
CTCGGTGCTGAGCCGGATTCTCTGGCTATCTCGGATGATGCACGGGAAATACCTCCGGATGGCTCTGAGGATGCGCCTGC
GGTGCGCCTTGTACGGGATGTCCTGCTTCGGGCTGTCAAGGAGGGGGCGTCCGATCTGCATTTCGAACCGCATGAGAAGT
ACTACCGGATACGTTGCCGTACCGATGGGCTTCTGTATGAGCTGGCACGACCTCCGATGTCTCTGGCCAGCAGGATATCG
ACACGCCTGAAGGTCATGGCCGGTATGGATATCGCGGAGCGTCGGCGGCCGCAGGATGGCCGGGCAAGGTTGGACGCGGC
CGATGGGCGCCCCGTCGACATGCGCGTCAGCACCCTGCCAGCCCTCTGGGGCGAACGAATCGTGATACGCATACTCGATG
CCTCCCTCTCCTGTCTGGGTGTCGATGAGTTGGGGCTGGAGGCAGAACAACAGCGGTTGTACCTGTCGGCATTGAGGCAA
TCACGGGGGCTGATTCTCGTGACCGGCCCCACCGGCTCTGGCAAGACAAGAACCTTGTACAGCGGGCTGGCTACGCTCAA
CACCGGAAGCCTGAATATATCGACGGTGGAAGATCCGGTGGAGATCAGCCTGGACGGTACCTGCCAGGTCAATATCAATC
CGCGCCAAGGGCTTGGCTTTGCCCAGGTATCCCGTGCACTCCTGCGGCAGGATCCGGATGTCATCATGGTGGGCGAGATT
CGCGATCCGGAAACAGCGGAAGTTGCCATCAAGGCGGCACAGACCGGCCATCTCGTCCTGTCCACATTGCATACCAACAG
TGCCGCGCAAACGCTGGAAAGACTGCGCAACCTGGGCATCCCGGCCTTCAACATCGCCGCAGCGGTCAGCCTGATCGTCG
CCCAACGCCTGGTACGCCGATTGTGCACGTGCAAGCGGGAAATATCGTTGCCACAGGAGGTGCTGCTTCAGGAGGGTTTC
CCGCCTGAGAGCATGGGATGTTTCACGCTTTTCGAAGGCGTGGGTTGCAGTGAATGCCGGGACGGTTACAGGGGGCGAAC
CGGCATACATGAGGTCGTACGGATCACTCCCGAGTTGCAGCAGTTGATCATGCGAGGAGGCAACTCCATCGAGATGGCCG
CCCTGGCCACGGCAGAAGGCTTTCACAGCCTGCGCGTTGCCGGCCTGCTGAAGGTCATGCAAGGCGTTACCAGCCTGTCT
GAAGTGAATCGAGTAATGGGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

48.75

98.765

0.481
  pilB Acinetobacter baylyi ADP1

48.387

98.413

0.476
  pilB Legionella pneumophila strain ERS1305867

46.384

100

0.464
  pilB Vibrio campbellii strain DS40M4

44.602

99.647

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

44.366

100

0.444
  pilB Vibrio cholerae strain A1552

48.617

89.242

0.434
  pilF Neisseria gonorrhoeae MS11

44.618

90.123

0.402