Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CQZ96_RS12070 Genome accession   NZ_CP058272
Coordinates   2585323..2587038 (+) Length   571 a.a.
NCBI ID   WP_105714638.1    Uniprot ID   -
Organism   Acinetobacter sp. MYb10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2580323..2592038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CQZ96_RS12030 (CQZ96_012060) nusA 2581065..2582549 (-) 1485 WP_004718337.1 transcription termination factor NusA -
  CQZ96_RS12035 (CQZ96_012065) rimP 2582584..2583108 (-) 525 WP_004718339.1 ribosome maturation factor RimP -
  CQZ96_RS12060 (CQZ96_012090) secG 2583900..2584241 (-) 342 WP_034613616.1 preprotein translocase subunit SecG -
  CQZ96_RS12065 (CQZ96_012095) tpiA 2584251..2585045 (-) 795 WP_105714639.1 triose-phosphate isomerase -
  CQZ96_RS12070 (CQZ96_012100) pilB 2585323..2587038 (+) 1716 WP_105714638.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CQZ96_RS12075 (CQZ96_012105) pilC 2587066..2588298 (+) 1233 WP_105714637.1 type II secretion system F family protein Machinery gene
  CQZ96_RS12080 (CQZ96_012110) pilD 2588301..2589182 (+) 882 WP_171287958.1 prepilin peptidase Machinery gene
  CQZ96_RS12085 (CQZ96_012115) coaE 2589209..2589808 (+) 600 WP_105714636.1 dephospho-CoA kinase -
  CQZ96_RS12090 (CQZ96_012120) - 2589877..2590788 (-) 912 WP_105714635.1 DMT family transporter -
  CQZ96_RS12095 (CQZ96_012125) rlmB 2590827..2591582 (-) 756 WP_105714634.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63541.99 Da        Isoelectric Point: 4.9143

>NTDB_id=463352 CQZ96_RS12070 WP_105714638.1 2585323..2587038(+) (pilB) [Acinetobacter sp. MYb10]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALSNAQKNKVSIVAYLSEKLNIPPLIIAEMISQEFGEPIFDLDSFNPDIL
PKELIEPNLVTKHKVIPLVQRGNILYVATSDPTNIEALDAIRFNCGLNIEPIIVEHHKLEKYLEKHFTVEESFNFGDEDF
DVDIIATDDNPPSEDDDDEASEEEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLAGRLA
SRLKVMSRMDISEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEKQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLSFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPVEIPRQSLLEMG
FTEQDLSNPDLQIFQPVGCNECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLIKVINGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=463352 CQZ96_RS12070 WP_105714638.1 2585323..2587038(+) (pilB) [Acinetobacter sp. MYb10]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGGTATATCCGTCGCTTAGTCGACGAAGGTTTTTTATCTGCCCAGCA
AATGAAAGATGCGCTCAGTAACGCTCAAAAAAATAAAGTGAGTATCGTGGCTTATCTCAGTGAAAAACTAAATATACCGC
CACTGATCATTGCTGAAATGATCTCCCAAGAATTTGGAGAACCCATTTTTGATCTTGATAGCTTTAATCCTGACATACTG
CCCAAGGAGCTTATTGAGCCAAATTTAGTCACTAAACATAAAGTGATTCCTTTAGTACAACGTGGCAATATTTTATATGT
CGCCACCAGTGATCCGACCAATATAGAAGCGCTGGATGCCATTCGTTTTAACTGCGGTTTGAATATTGAACCGATCATTG
TTGAACATCATAAATTAGAAAAATATTTAGAAAAGCACTTTACTGTAGAGGAAAGCTTTAATTTTGGTGATGAAGATTTT
GACGTTGATATCATTGCCACTGATGACAACCCTCCATCAGAAGATGATGACGATGAGGCTTCAGAAGAAGAAGCACCTAT
TGTTAAATATATCAATAAACTCTTGATCGATGCGATTCGTATGGGTGCATCTGATTTACACTTTGAACCTTATGAAAAAA
GTTATCGGGTGCGTTATCGTGTAGATGGGGTACTAAGAACCATTACCAATCCCCCCTTGCAATTAGCTGGCCGTTTGGCA
TCACGTCTAAAAGTGATGTCTAGAATGGATATTTCTGAAAAGCGCGTTCCGCAGGATGGTCGTATTAAGTTAAAAATATC
AAAGACCAAAGCCATTGATTTTCGTGTGAATTCACTACCGACATTATTTGGCGAAAAATTGGTACTGCGTATTCTTGACC
CTTCCAGCGCAATGTTGGGTATTGATGCGCTGGGTTATGAAGAAAAGCAAAAAGCATTATTTATGGAGGCCTTAGAAAAA
CCTCAAGGCATGTTACTGATTACTGGACCCACAGGTTCAGGTAAAACTGTTTCCCTCTATACGGGCTTAAATATCCTCAA
TACAGAAAGTTCAAATATTTCTACAGCCGAAGATCCTGTTGAAATTAACTTGGAAGGGATCAATCAAGTTAACGTCAATC
TTAAAGCAGGTCTCAGCTTTTCGGTCGCTTTGAAATCATTCCTACGTCAAGATCCTGACATTATCATGGTCGGTGAGATT
CGAGACCTCGAAACTGCTGAAATTGCGATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCTACCCTACATACCAACAG
CGCACCTGAAACATTAACCCGTTTACGTAATATGGGTGTACCTTCATTTAATATTGCCACCTCAGTCAATCTTGTGATTG
CGCAACGATTGGCAAGACGTTTATGTAATCATTGTAAAGCACCCGTTGAAATCCCTAGACAGAGTCTGTTAGAAATGGGC
TTTACCGAACAAGACTTAAGCAATCCTGACTTACAAATTTTTCAGCCTGTGGGCTGTAATGAATGCCGTGAAGGCTATAA
AGGTCGTGTCGGGATCTACGAAGTGATGAAAGTAACACCTGAGATTTCAAAAATTATCATGGAAGATGGCAATGCGCTGC
AAATTGCAGCAGCATCGGAGAAATTAGGGTTTGATAATCTAAGACGTTCAGGTTTAATTAAAGTCATCAATGGGTTAACT
TCTTTACAAGAAATTAACCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.846

100

0.79
  pilB Acinetobacter baylyi ADP1

75.131

100

0.751
  pilB Legionella pneumophila strain ERS1305867

50.871

100

0.511
  pilF Neisseria gonorrhoeae MS11

49.823

98.774

0.492
  pilB Vibrio campbellii strain DS40M4

47.594

98.249

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.772

90.718

0.461
  pilB Vibrio cholerae strain A1552

50.677

90.543

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

34.584

100

0.371