Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HA399_RS03735 Genome accession   NZ_CP058244
Coordinates   879047..880783 (-) Length   578 a.a.
NCBI ID   WP_404326097.1    Uniprot ID   -
Organism   Cobetia sp. UIB-001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 874047..885783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HA399_RS03710 (HA399_03710) - 874713..875666 (+) 954 WP_107335407.1 Nudix family hydrolase -
  HA399_RS03715 (HA399_03715) yacG 875797..876042 (-) 246 WP_043332481.1 DNA gyrase inhibitor YacG -
  HA399_RS03720 (HA399_03720) coaE 876035..876685 (-) 651 WP_404326093.1 dephospho-CoA kinase -
  HA399_RS03725 (HA399_03725) pilD 876682..877542 (-) 861 WP_303568750.1 A24 family peptidase Machinery gene
  HA399_RS03730 (HA399_03730) pilC 877634..878872 (-) 1239 WP_404326095.1 type II secretion system F family protein Machinery gene
  HA399_RS03735 (HA399_03735) pilB 879047..880783 (-) 1737 WP_404326097.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HA399_RS03740 (HA399_03740) - 881358..881804 (+) 447 WP_404326099.1 pilin -
  HA399_RS03745 (HA399_03745) - 882025..883200 (+) 1176 WP_404326101.1 O-antigen ligase family protein -
  HA399_RS03750 (HA399_03750) - 883238..883540 (-) 303 WP_043332474.1 hypothetical protein -
  HA399_RS03755 (HA399_03755) - 883713..884999 (-) 1287 WP_404326102.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63454.54 Da        Isoelectric Point: 4.8793

>NTDB_id=463004 HA399_RS03735 WP_404326097.1 879047..880783(-) (pilB) [Cobetia sp. UIB-001]
MPQSEHTLKGFAARLVDSGLLEPQAAQLAEQEANEAGESLLLHIIAHGLVTARDATLAAAWEYGLPYVDLDALRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLTPALEQYLEAGEESAMDMLSED
GEALDGLDFEGEEDNNHDMAITASSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLIENTRPPFNMRN
RISARLKVMSRMDISERRLPQDGAIKLRLSKSRSIDFRVNTLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LDQPQGMILVTGPTGSGKTVTLYTGLNILNTPERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCTHCRQPAEIPPEAMA
SMGFSEDNGFSAAELAAATIFRPVGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGTAIELADQARKEGLSDLRRSGLR
KVMSGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=463004 HA399_RS03735 WP_404326097.1 879047..880783(-) (pilB) [Cobetia sp. UIB-001]
ATGCCCCAGTCAGAACACACGCTAAAAGGATTTGCTGCACGACTGGTCGACTCTGGCCTGCTCGAACCACAGGCAGCACA
GCTGGCTGAACAGGAAGCCAATGAGGCCGGAGAAAGCCTGTTGCTGCATATCATCGCGCACGGCCTGGTGACGGCACGTG
ACGCGACATTGGCGGCCGCCTGGGAATACGGCTTACCGTATGTGGATCTCGATGCCCTTCGTATCGACTCGCTGCCTTCC
ATTGATGAGCTGCCCGAGAAAGTCTTGCGCAAGCTGGGAGTATTGCCACTCTATCGCCGCGAGCATCGCCTGACCGTCGG
TGTGCCCTCCCCATCGACGCTCACGCATCTTGATGAACTGCAATTTGCCACCGGCTTGTCGATCGAGAGTGTCCTAGTCG
CCGCCGACCAGCTGACGCCGGCATTGGAGCAGTACCTGGAGGCCGGCGAAGAAAGCGCCATGGACATGCTGAGCGAAGAT
GGCGAAGCGCTTGATGGGCTGGATTTCGAGGGCGAAGAAGATAACAACCATGACATGGCGATCACGGCCAGCAGTGACGA
TGCACCGGTGGTGCGCTTCGTTCACAAGGTGCTGCTGGATGCCATCAAGCGTGGCGCCTCGGATATCCACTTCGAGCCCT
ACGAATCCACCTATCGCATTCGTTTTCGTATCGATGGCATGTTGATCGAGAATACTCGCCCGCCCTTCAATATGCGCAAT
CGTATCTCGGCGCGCCTGAAAGTCATGTCGCGCATGGATATTTCCGAGCGCCGTTTGCCGCAGGATGGTGCAATCAAATT
GCGTCTGTCCAAGTCCCGCTCCATCGACTTCCGTGTCAATACACTGCCCACCGTGTACGGCGAGAAGCTGGTGCTGCGTA
TCCTCGACCCCAGCTCGGCCAAGATGGGCATCGAGGCGCTGGGATTTGAACCCGAGCAACGCGCCATGTTCGAGAAGACG
CTGGATCAGCCACAGGGCATGATTCTGGTCACTGGCCCGACCGGTAGCGGCAAGACCGTGACGCTCTACACCGGCTTGAA
TATCCTCAATACACCGGAGCGCAATATCTCGACGGCGGAAGATCCGGTCGAGCTCAAGGTAGAAGGCGTCAACCAGGTCA
ACGTGCTGCCCAAGATCGGCCTGGACTTCGCTTCGGCCCTGCGCGCCTTCCTGCGCCAGGATCCCGATGTGGTGATGGTC
GGGGAAATTCGTGACAAGGAAACCGCCGAGATTGCCGTCAAGGCGGCACAGACCGGTCACCTGGTACTTTCTACCTTGCA
CACCAACTCGGCGCCCGAGACACTGACACGCCTGAGCAACATGGGAATTGCCCCCTTCAATATCGCGAGTTCCGTCTCGT
TGATCATCGCTCAGCGCCTTGCTCGCCGCCTGTGTACCCACTGCCGACAGCCCGCGGAGATTCCGCCCGAGGCGATGGCC
TCGATGGGCTTCAGTGAAGACAATGGCTTCAGTGCAGCAGAACTCGCGGCCGCAACCATATTCCGCCCCGTCGGTTGCAG
CCAGTGCACGCTGGGTTACAAGGGGCGAGTCGGCATCTATGAAGTCATGCCGATCTCGGATGAGATGAGCCGCTTGATCA
TGCGTGAGGGCACCGCCATCGAGCTGGCAGACCAGGCGCGCAAGGAAGGGCTTTCAGACCTGAGGCGTAGCGGCTTGCGC
AAGGTGATGTCGGGCATGACCTCGCTGGAAGAAGTCAATCGCATCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.715

98.789

0.521
  pilB Legionella pneumophila strain ERS1305867

51.91

99.654

0.517
  pilB Acinetobacter baylyi ADP1

52.098

98.962

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

48.325

98.097

0.474
  pilB Vibrio cholerae strain A1552

47.14

99.827

0.471
  pilB Vibrio campbellii strain DS40M4

47.12

99.135

0.467
  pilF Neisseria gonorrhoeae MS11

47.662

96.194

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.368

99.654

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

41.825

91.003

0.381
  pilF Thermus thermophilus HB27

38.407

97.751

0.375