Detailed information NTG0458632

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV347_RS20740
• Genome accession: NZ_CP056191
• Coordinates: 4233059..4233859 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain RHBSTW-00995
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4228059..4238859

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV347_RS20705 (HV347_20710)	gspH	4228215..4228730 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
HV347_RS20710 (HV347_20715)	gspI	4228727..4229092 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HV347_RS20715 (HV347_20720)	gspJ	4229089..4229685 (+)	597	WP_023279845.1	type II secretion system minor pseudopilin GspJ	-
HV347_RS20720 (HV347_20725)	gspK	4229682..4230668 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
HV347_RS20725 (HV347_20730)	gspL	4230655..4231848 (+)	1194	WP_025985182.1	type II secretion system protein GspL	-
HV347_RS20730 (HV347_20735)	-	4231841..4232326 (+)	486	WP_004145896.1	type II secretion system protein M	-
HV347_RS20735 (HV347_20740)	-	4232316..4233062 (+)	747	WP_009309263.1	type II secretion system protein N	-
HV347_RS20740 (HV347_20745)	pilD	4233059..4233859 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
HV347_RS20745 (HV347_20750)	hrpB	4233907..4236336 (-)	2430	WP_004222692.1	ATP-dependent helicase HrpB	-
HV347_RS20750 (HV347_20755)	thpR	4236409..4236945 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HV347_RS20755 (HV347_20760)	sfsA	4236945..4237661 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HV347_RS20760 (HV347_20765)	dksA	4237824..4238279 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=458632 HV347_RS20740 WP_004145898.1 4233059..4233859(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00995]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=458632 HV347_RS20740 WP_004145898.1 4233059..4233859(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00995]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGGCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425