Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HWQ56_RS02340 Genome accession   NZ_CP056030
Coordinates   487661..489733 (+) Length   690 a.a.
NCBI ID   WP_158154469.1    Uniprot ID   A0A7D5D4D8
Organism   Pseudomonas eucalypticola strain NP-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482661..494733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ56_RS02320 (HWQ56_02320) comM 484812..485876 (+) 1065 WP_158154473.1 pilus assembly protein PilM Machinery gene
  HWQ56_RS02325 (HWQ56_02325) - 485876..486448 (+) 573 WP_158154472.1 PilN domain-containing protein -
  HWQ56_RS02330 (HWQ56_02330) pilO 486445..487104 (+) 660 WP_158154471.1 type 4a pilus biogenesis protein PilO -
  HWQ56_RS02335 (HWQ56_02335) - 487101..487625 (+) 525 WP_158154470.1 pilus assembly protein PilP -
  HWQ56_RS02340 (HWQ56_02340) pilQ 487661..489733 (+) 2073 WP_158154469.1 type IV pilus secretin PilQ Machinery gene
  HWQ56_RS02345 (HWQ56_02345) aroK 489738..490256 (+) 519 WP_158154468.1 shikimate kinase AroK -
  HWQ56_RS02350 (HWQ56_02350) aroB 490387..491484 (+) 1098 WP_158154467.1 3-dehydroquinate synthase -
  HWQ56_RS02355 (HWQ56_02355) - 491499..493109 (+) 1611 WP_176569688.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74320.13 Da        Isoelectric Point: 5.0733

>NTDB_id=457952 HWQ56_RS02340 WP_158154469.1 487661..489733(+) (pilQ) [Pseudomonas eucalypticola strain NP-1]
MNRMLSALGLTLWMALLSPAVMAATLKTLDVAALPGDRVELKLTFDTPVPAPRGYTTDQPARIALDLPGVNNGLSTKTRD
LGVGNARSVTVVQASDRTRLIINLTNLAPYGTRVEGNNLYVMIGQGANSLEGNAAAPAGASVAPRAAVASAAPSRPAYAQ
PAGRAIRNVDFQRGAQGEGNVVIDLSDATLSPDIQEQGNKIVLTFPKTQLPDPLRVRLDVKDFATPVQFVNSTVSADTAT
VTIEPGGTFDYSVFQTDNKLTVSVREVTAEDIARKNADRFVYTGQKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGG
ITLRLQNVPWDQALDLVLKTKNLDKRKVGNVLLVAPAAEIAARERQELESQKQIEELAPLRRELLQVNYAKAADIAKLFQ
SVTNAKATTDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVNYDKSLGVSWSGATNGTGN
NRWTVAGTQTTSTNTFVDLGATSATSGIGLGYVSNNLTLDLQLSAMEASGNGEVVSQPKVVTSDKETARIIKGTEVPYQE
SSSSGATSVSFKEASLSLEVTPQITPDNRIIMDVKVNKDEPDYANELNGVPPIKKNEVKAKVLVNDGETVVIGGVFSNTQ
TKTTAKVPFLGDVPYLGRLFRSDTVSEEKTELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=457952 HWQ56_RS02340 WP_158154469.1 487661..489733(+) (pilQ) [Pseudomonas eucalypticola strain NP-1]
ATGAATAGGATGCTTTCAGCCCTTGGTCTCACGCTATGGATGGCGCTGCTTTCGCCCGCCGTGATGGCGGCCACCCTGAA
AACGCTGGATGTGGCGGCGTTGCCGGGGGACCGGGTCGAGCTCAAGCTGACTTTCGATACCCCGGTGCCGGCCCCACGCG
GCTACACCACCGACCAGCCCGCCCGTATCGCCCTGGACCTTCCCGGCGTGAACAACGGGCTGTCCACCAAAACCCGTGAC
CTGGGCGTGGGCAATGCCCGTAGCGTCACCGTGGTGCAGGCCAGCGACCGTACCCGGCTGATCATCAACCTGACCAACCT
GGCGCCCTATGGCACTCGGGTTGAAGGCAACAACCTGTACGTGATGATCGGCCAGGGCGCCAATAGCCTGGAGGGCAATG
CAGCGGCCCCTGCGGGCGCCAGCGTGGCGCCACGCGCGGCCGTCGCCAGCGCGGCGCCTTCGCGGCCGGCCTATGCCCAA
CCGGCTGGCCGGGCCATTCGCAATGTCGATTTCCAGCGCGGTGCCCAGGGCGAAGGCAATGTGGTCATCGACCTCAGCGA
TGCCACCCTCAGTCCCGATATCCAGGAGCAGGGCAACAAGATCGTCCTGACGTTCCCCAAGACCCAGCTGCCAGACCCTT
TGCGGGTGCGCCTGGACGTCAAGGATTTCGCCACGCCGGTGCAGTTCGTCAACTCGACCGTTTCGGCTGACACCGCCACC
GTTACCATCGAGCCGGGTGGCACCTTCGATTATTCGGTGTTCCAGACCGACAACAAGCTGACCGTCAGCGTGCGTGAGGT
GACCGCCGAGGACATCGCCCGCAAGAATGCCGACCGTTTTGTGTACACCGGCCAGAAGCTGTCGCTCAACTTCCAGGATA
TCGACGTGCGCTCGGTGCTGCAGTTGATCGCGGACTTCACCAACCTCAACCTGGTGGCCAGCGACACCGTTCAGGGCGGC
ATCACCCTGCGCCTGCAGAATGTGCCGTGGGACCAGGCCCTGGATCTGGTGCTCAAGACCAAGAACCTGGACAAGCGCAA
GGTGGGCAACGTGCTGCTGGTGGCCCCGGCCGCCGAGATCGCCGCGCGTGAGCGTCAGGAGCTGGAGTCGCAGAAGCAGA
TCGAGGAACTGGCGCCCCTGCGCCGTGAACTGTTGCAGGTCAACTATGCCAAGGCGGCCGACATCGCCAAACTGTTCCAG
TCGGTCACCAATGCCAAGGCCACGACCGATGAGCGCGGCTCGATTACCGTGGATGAGCGCACCAACAACATCATCGCTTA
CCAGACCCAGGACCGCCTCGACGAACTGCGGCGCATCGTTGCGCAATTGGATATTCCGGTTCGCCAGGTCATGATCGAGG
CGCGCATCGTCGAGGCCAATGTCAACTACGACAAGAGCCTGGGCGTGAGCTGGAGCGGCGCCACCAATGGGACTGGCAAC
AACCGCTGGACCGTCGCCGGCACCCAGACCACCAGCACCAACACCTTCGTCGACCTGGGCGCCACCAGCGCCACCTCGGG
TATCGGCCTGGGCTACGTGTCCAACAACCTGACACTGGACCTGCAGCTCAGCGCGATGGAGGCCAGCGGCAACGGCGAAG
TGGTGTCGCAGCCCAAGGTCGTGACCTCCGACAAGGAAACCGCGCGGATCATCAAGGGTACCGAAGTGCCGTACCAGGAA
TCCTCTTCCAGCGGCGCTACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACACCGCAGATCACCCCTGA
CAACCGCATCATCATGGATGTGAAGGTCAACAAGGATGAACCTGACTATGCCAACGAGCTCAACGGCGTACCGCCCATCA
AGAAGAACGAGGTGAAGGCGAAGGTCCTCGTCAATGATGGCGAAACAGTGGTAATCGGGGGTGTTTTCTCCAATACCCAA
ACAAAAACCACCGCGAAGGTGCCATTTCTTGGTGATGTGCCGTATCTTGGCCGCCTGTTTCGATCGGATACCGTGTCCGA
GGAAAAAACCGAGCTGTTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(310-356)

Bacterial type II/III secretion system short domain (Secretin_N)

(384-449)

AMIN domain (AMIN)

(42-121)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5D4D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.897

100

0.745
  comQ Acinetobacter baylyi ADP1

42.06

100

0.426
  pilQ Acinetobacter baumannii D1279779

38.544

100

0.414
  pilQ Acinetobacter baumannii strain A118

38.544

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.053

98.261

0.374
  pilQ Legionella pneumophila strain Lp02

38.053

98.261

0.374