Detailed information NTG0457951

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: HWQ56_RS02320
• Genome accession: NZ_CP056030
• Coordinates: 484812..485876 (+)
• Length: 354 a.a.
• NCBI ID: WP_158154473.1
• Uniprot ID: A0A7D5D4L0
• Organism: Pseudomonas eucalypticola strain NP-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 479812..490876

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWQ56_RS02305 (HWQ56_02305)	rpmE	480417..480635 (+)	219	WP_158154476.1	50S ribosomal protein L31	-
HWQ56_RS02310 (HWQ56_02310)	-	480876..482144 (+)	1269	WP_158154475.1	malic enzyme-like NAD(P)-binding protein	-
HWQ56_RS02315 (HWQ56_02315)	-	482206..484629 (-)	2424	WP_176569687.1	penicillin-binding protein 1A	-
HWQ56_RS02320 (HWQ56_02320)	comM	484812..485876 (+)	1065	WP_158154473.1	pilus assembly protein PilM	Machinery gene
HWQ56_RS02325 (HWQ56_02325)	-	485876..486448 (+)	573	WP_158154472.1	PilN domain-containing protein	-
HWQ56_RS02330 (HWQ56_02330)	pilO	486445..487104 (+)	660	WP_158154471.1	type 4a pilus biogenesis protein PilO	-
HWQ56_RS02335 (HWQ56_02335)	-	487101..487625 (+)	525	WP_158154470.1	pilus assembly protein PilP	-
HWQ56_RS02340 (HWQ56_02340)	pilQ	487661..489733 (+)	2073	WP_158154469.1	type IV pilus secretin PilQ	Machinery gene
HWQ56_RS02345 (HWQ56_02345)	aroK	489738..490256 (+)	519	WP_158154468.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37757.27 Da
• Isoelectric Point: 4.4324

>NTDB_id=457951 HWQ56_RS02320 WP_158154473.1 484812..485876(+) (comM) [Pseudomonas eucalypticola strain NP-1]
MLGLFGKKANSLLGIDISSTSVKLLELSRSGNRYKVEAYAVEPLPANAVVEKNIAELEGVGQALARVLVKARSSAKTAAV
AVAGSAVITKTIEMDAALSEDEMESQLKVEADQYIPYPLEEVAIDFEVQGYSARNPERVDVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLEPALVDGDGPLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVMEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASISGLDQLIQQRLGTPAMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=457951 HWQ56_RS02320 WP_158154473.1 484812..485876(+) (comM) [Pseudomonas eucalypticola strain NP-1]
GTGCTTGGACTCTTCGGCAAGAAAGCGAATTCGCTTCTGGGGATCGACATCAGCTCTACATCGGTAAAGCTTCTGGAGCT
CAGCCGCTCCGGCAACCGATACAAGGTCGAGGCCTACGCGGTTGAGCCCCTCCCGGCCAACGCGGTGGTGGAAAAGAACA
TTGCGGAACTCGAAGGCGTGGGCCAGGCCCTGGCACGCGTGCTGGTCAAGGCGCGCAGCAGCGCCAAGACCGCCGCCGTG
GCGGTGGCCGGCTCGGCCGTGATCACCAAGACCATTGAAATGGACGCCGCGCTTTCCGAAGACGAAATGGAAAGCCAGCT
CAAGGTCGAGGCCGATCAGTACATTCCCTACCCCCTGGAAGAGGTCGCCATCGACTTCGAAGTGCAGGGCTATTCGGCGC
GCAACCCGGAGCGGGTGGACGTGCTGCTGGCGGCTTGCCGCAAGGAAAACGTCGAAGTGCGCGAAGCCGCCCTGGCCCTG
GCGGGCCTCACCGCGCGCGTGGTCGACGTCGAGGCCTATGCCCTGGAACGGGCCTTCGGTTTGCTGGAGCCGGCCCTGGT
GGATGGCGATGGGCCGCTGACCGTGGCCGTGGTGGACATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGCC
GTATCATCTACACCCGCGAGCAGTTGTTCGGCGGCCGCCAGCTGACCGAGGAAATCCAGCGCCGTTACGGCCTGTCGATG
GAAGAGGCTGGCCTGGCCAAGAAGCAGGGTGGGCTGCCCGACGACTACGTGATGGAAGTGCTGCAACCGTTCAAGGATGC
GGTGGTGCAGCAGGTGTCGCGTTCCCTGCAGTTCTTCTTCGCCGCCGGGCAGTTCAATGACGTCGACTACATCCTGTTGG
CGGGGGGCACGGCCTCCATCTCAGGTCTGGACCAGTTGATCCAGCAGCGCCTGGGCACCCCGGCGATGGTGGCCAACCCC
TTCGCCGACATGGCCCTGAGCAGCAAGGTCAACGCCGGCGCCCTGGCCAGCGATGCGCCAGCGCTGATGATCGCCTGCGG
CCTGGCCTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A7D5D4L0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	57.91	100	0.579
pilM	Acinetobacter baumannii D1279779	57.91	100	0.579
comM	Acinetobacter baylyi ADP1	54.52	100	0.545
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46