Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HU689_RS02360 Genome accession   NZ_CP055159
Coordinates   519479..521185 (-) Length   568 a.a.
NCBI ID   WP_186265260.1    Uniprot ID   -
Organism   Shewanella algae strain 2NE11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514479..526185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU689_RS02325 (HU689_02325) - 514715..514982 (-) 268 Protein_430 transposase -
  HU689_RS02330 (HU689_02330) mutT 515060..515458 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  HU689_RS02335 (HU689_02335) yacG 515458..515670 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  HU689_RS02340 (HU689_02340) zapD 515715..516455 (-) 741 WP_028779562.1 cell division protein ZapD -
  HU689_RS02345 (HU689_02345) coaE 516452..517063 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  HU689_RS02350 (HU689_02350) pilD 517064..517978 (-) 915 WP_096142092.1 prepilin peptidase Machinery gene
  HU689_RS02355 (HU689_02355) pilC 518053..519321 (-) 1269 WP_096142091.1 type II secretion system F family protein Machinery gene
  HU689_RS02360 (HU689_02360) pilB 519479..521185 (-) 1707 WP_186265260.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HU689_RS22530 (HU689_02365) - 521254..521595 (-) 342 WP_096142089.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HU689_RS02370 (HU689_02370) nadC 522454..523314 (-) 861 WP_096142088.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HU689_RS02375 (HU689_02375) ampD 523473..524051 (+) 579 WP_186265262.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HU689_RS02380 (HU689_02380) ampE 524105..524956 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  HU689_RS02385 (HU689_02385) pdhR 525232..525984 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63016.17 Da        Isoelectric Point: 5.4623

>NTDB_id=456455 HU689_RS02360 WP_186265260.1 519479..521185(-) (pilB) [Shewanella algae strain 2NE11]
MPTSSVHLGLSTLFIRKGLLSEEELAKAISFSKNQKQSLISTIIQNGALSARAIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLGKALEKVLEEDLSSLDLNGLDEES
LAGIEVTDTDKRHQEESESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQINTGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=456455 HU689_RS02360 WP_186265260.1 519479..521185(-) (pilB) [Shewanella algae strain 2NE11]
ATGCCGACATCGTCAGTTCATTTGGGATTATCAACACTTTTCATTCGCAAAGGACTACTAAGCGAAGAGGAACTAGCAAA
GGCGATTAGTTTTTCAAAAAATCAGAAACAAAGTTTAATTTCAACGATCATTCAGAATGGTGCTTTGTCGGCAAGGGCAA
TTGCAGAACTGTGTTATGAGGAATACGGTACTCCACTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATTCCGGAAGAG
TTTCTCAACAAAAAACTGATCGAAAAGCACCGCTGCCTGCCGTTGTTCAAACGCGGCAACCGCCTCTATATCGCCACTTC
AGACCCCACCAATATCGCCGCCCTGGAGGACTTTCAGTTCAGCGCCGGCCTGCACGCGGAAGCGATACTGGTCGAAGAGG
ATAAGCTAGGTAAAGCACTGGAAAAGGTGCTTGAAGAGGACTTGAGTTCACTGGATCTCAATGGCCTCGATGAGGAATCC
CTCGCCGGTATCGAGGTCACAGATACCGACAAGCGCCACCAGGAAGAGAGCGAAAGTAAAGATGACGCTCCCATAGTCAT
CTATATCAACAAGATTCTAACCGATGCCATCCGTAAGGGCGCATCGGATCTGCATTTCGAGCCTTACGAAAAACGCTACC
GCATTCGCTTTCGTATCGATGGCATCCTCCACGAAGTTTCCGAACCACCCATTTCTCTCTCGGGCCGTATCTCTGCCCGC
TTGAAAGTGATGTCCAAATTGGATATCGCCGAGCGCCGGGTACCCCAGGATGGCCGCATCAAAATGAAGCTGTCGCGTAA
CAAGAGTATCGATTTCCGGGTAAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTTATGCGGATCCTCGACTCCTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTAACCGGCCCTACCGGCTCTGGTAAAACCGTGTCCCTCTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGATCCGGTAGAGATCAACCTGGAAGGGGTCAACCAGGTGCATATCAACCCCAAGG
CAGGGCTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTGGAAACCGCCGAAATCGCCATTAAGGCGGCACAAACCGGCCACTTGGTACTCTCAACCCTGCATACCAACTCATCGGC
CGAAACCCTTACCCGCCTGCTTAATATGGGTGTTCCAGGTTATAACATTGCCAGTTCTGTCAATCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAAATAAACACAGGCTTTACCCCATATAAGCCCTGCGGCTGCGAATTCTGCTCCGGTGGCTATAAGGGCCGGGT
CGGTATCTATGAGGTGATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCG
CTACCGCCAAGCAGCAAGGGATGCGCGACCTGCGCCTTTCAGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.537

98.768

0.588
  pilB Acinetobacter baumannii D1279779

58.73

99.824

0.586
  pilB Legionella pneumophila strain ERS1305867

56.007

99.648

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

52.566

99.472

0.523
  pilB Vibrio cholerae strain A1552

52.962

98.063

0.519
  pilB Vibrio campbellii strain DS40M4

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

47.957

99.12

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

37.766

99.296

0.375