Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HUT27_RS02325 Genome accession   NZ_CP054867
Coordinates   517585..519657 (+) Length   690 a.a.
NCBI ID   WP_124354532.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain PCLRT02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512585..524657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUT27_RS02305 (HUT27_02305) pilM 514815..515867 (+) 1053 WP_124354531.1 pilus assembly protein PilM Machinery gene
  HUT27_RS02310 (HUT27_02310) - 515867..516433 (+) 567 WP_009046598.1 PilN domain-containing protein -
  HUT27_RS02315 (HUT27_02315) pilO 516430..517047 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  HUT27_RS02320 (HUT27_02320) - 517044..517571 (+) 528 WP_041985501.1 pilus assembly protein PilP -
  HUT27_RS02325 (HUT27_02325) pilQ 517585..519657 (+) 2073 WP_124354532.1 type IV pilus secretin PilQ Machinery gene
  HUT27_RS02330 (HUT27_02330) aroK 519662..520180 (+) 519 WP_009041824.1 shikimate kinase AroK -
  HUT27_RS02335 (HUT27_02335) aroB 520328..521428 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  HUT27_RS02340 (HUT27_02340) - 521439..523028 (+) 1590 WP_009046603.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74685.80 Da        Isoelectric Point: 5.4337

>NTDB_id=455054 HUT27_RS02325 WP_124354532.1 517585..519657(+) (pilQ) [Pseudomonas chlororaphis strain PCLRT02]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGPRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATVAQSAPGQPAVASGRAKATASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNTERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=455054 HUT27_RS02325 WP_124354532.1 517585..519657(+) (pilQ) [Pseudomonas chlororaphis strain PCLRT02]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCGCTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGACCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCCGCGTACCCGGGTGGTGGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGAGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGTGGCGCAGAGTGCAC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGGCCAAGGCCACGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTCCAG
CGCGGCGAGCAGGGTGAAGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGTCTGCCAGAACCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTAAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACACCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAAAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGATCTCGAGCCGCTGCGGCGTGAACTGCTGC
AGGTCAACTATGCCAAGGCGGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCTGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATTGGTACGCCGGGCAGCACCAGCAGCAATTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAAACCGGTAACGGCGAAA
TCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGTGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.049

100

0.736
  comQ Acinetobacter baylyi ADP1

39.754

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.852

100

0.409
  pilQ Acinetobacter baumannii D1279779

37.852

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37