Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HUT27_RS02305 Genome accession   NZ_CP054867
Coordinates   514815..515867 (+) Length   350 a.a.
NCBI ID   WP_124354531.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain PCLRT02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509815..520867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUT27_RS02295 (HUT27_02295) - 510767..512035 (+) 1269 WP_009046595.1 malic enzyme-like NAD(P)-binding protein -
  HUT27_RS02300 (HUT27_02300) - 512163..514598 (-) 2436 WP_164485464.1 penicillin-binding protein 1A -
  HUT27_RS02305 (HUT27_02305) pilM 514815..515867 (+) 1053 WP_124354531.1 pilus assembly protein PilM Machinery gene
  HUT27_RS02310 (HUT27_02310) - 515867..516433 (+) 567 WP_009046598.1 PilN domain-containing protein -
  HUT27_RS02315 (HUT27_02315) pilO 516430..517047 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  HUT27_RS02320 (HUT27_02320) - 517044..517571 (+) 528 WP_041985501.1 pilus assembly protein PilP -
  HUT27_RS02325 (HUT27_02325) pilQ 517585..519657 (+) 2073 WP_124354532.1 type IV pilus secretin PilQ Machinery gene
  HUT27_RS02330 (HUT27_02330) aroK 519662..520180 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37058.49 Da        Isoelectric Point: 4.9975

>NTDB_id=455053 HUT27_RS02305 WP_124354531.1 514815..515867(+) (pilM) [Pseudomonas chlororaphis strain PCLRT02]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAS
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQHLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=455053 HUT27_RS02305 WP_124354531.1 514815..515867(+) (pilM) [Pseudomonas chlororaphis strain PCLRT02]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTTATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCAGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCTATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCACCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.682

100

0.56
  comM Acinetobacter nosocomialis M2

55.398

100

0.557
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46