Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUK68_RS15715 Genome accession   NZ_CP054840
Coordinates   3394761..3396506 (+) Length   581 a.a.
NCBI ID   WP_175505033.1    Uniprot ID   A0A6N1X7F7
Organism   Comamonas antarctica strain 16-35-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3389761..3401506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK68_RS15685 (HUK68_15685) - 3390056..3390376 (+) 321 WP_175505028.1 metalloregulator ArsR/SmtB family transcription factor -
  HUK68_RS15690 (HUK68_15690) - 3390376..3390810 (+) 435 WP_175505029.1 YeeE/YedE family protein -
  HUK68_RS15695 (HUK68_15695) - 3390821..3391240 (+) 420 WP_175505030.1 DUF6691 family protein -
  HUK68_RS15700 (HUK68_15700) - 3391232..3391951 (-) 720 WP_244146187.1 transcriptional regulator -
  HUK68_RS15705 (HUK68_15705) - 3391985..3394075 (-) 2091 WP_175505031.1 aminotransferase class V-fold PLP-dependent enzyme -
  HUK68_RS15710 (HUK68_15710) - 3394173..3394640 (+) 468 WP_175505032.1 Lrp/AsnC family transcriptional regulator -
  HUK68_RS15715 (HUK68_15715) pilB 3394761..3396506 (+) 1746 WP_175505033.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK68_RS15720 (HUK68_15720) pilC 3396524..3397732 (+) 1209 WP_175505034.1 type II secretion system F family protein Machinery gene
  HUK68_RS15725 (HUK68_15725) pilD 3397738..3398619 (+) 882 WP_175505867.1 A24 family peptidase Machinery gene
  HUK68_RS15730 (HUK68_15730) coaE 3398619..3399254 (+) 636 WP_175505035.1 dephospho-CoA kinase -
  HUK68_RS15735 (HUK68_15735) zapD 3399378..3400133 (+) 756 WP_175505036.1 cell division protein ZapD -
  HUK68_RS15740 (HUK68_15740) - 3400141..3400353 (+) 213 WP_175505037.1 DNA gyrase inhibitor YacG -
  HUK68_RS15745 (HUK68_15745) - 3400363..3400824 (-) 462 WP_175505038.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63212.34 Da        Isoelectric Point: 5.7361

>NTDB_id=454845 HUK68_RS15715 WP_175505033.1 3394761..3396506(+) (pilB) [Comamonas antarctica strain 16-35-5]
MPAADSVTPSTSSMALPGLGRALISAGTITAQVAEDLHRKSQAARTSFISELIGSGTVTSAQVAHTLSSVFGAPLIDLDA
IDPALLPQGLLAPKLCLNYRVVVLSKRNNRLTIAAADPTDQEAAERIKFTSQMGIDWIIAEYDKLVQLCESMHKSVSESL
QSYTSSGDFNFDDIKAEEAEEANDSANTAGSDVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRVDGELREIA
SPPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPNRTIDFRVSTLPTLFGEKIVIRILDPESAKMGIEALGYEPE
EKDRLMHAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLR
QDPDIIMVGEIRDLETADTSIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPA
DIPREALLDAGYDANDLDGSWVTYKPVGCKACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKLEGVRSLRDS
GLYKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=454845 HUK68_RS15715 WP_175505033.1 3394761..3396506(+) (pilB) [Comamonas antarctica strain 16-35-5]
ATGCCCGCTGCTGACTCCGTCACTCCGTCCACATCCAGCATGGCGCTTCCCGGCCTGGGACGCGCGTTGATTTCCGCCGG
CACGATCACGGCCCAGGTGGCCGAGGATCTGCACCGCAAATCCCAGGCTGCGCGCACCAGCTTCATCTCCGAACTCATAG
GTTCCGGCACCGTGACGTCGGCGCAGGTGGCGCATACGCTGTCGAGCGTCTTCGGCGCGCCGCTGATCGATCTCGACGCC
ATCGATCCCGCATTGCTGCCCCAGGGGCTGCTTGCGCCCAAGCTCTGCCTGAACTACCGCGTGGTGGTGCTGAGCAAGCG
CAACAACCGCCTGACGATTGCCGCCGCCGATCCCACCGACCAGGAGGCCGCGGAACGCATCAAGTTCACCTCCCAGATGG
GGATCGACTGGATCATTGCCGAGTACGACAAGCTGGTGCAACTGTGCGAGTCGATGCACAAGAGCGTTTCCGAATCGCTG
CAGTCCTATACCTCGTCGGGTGACTTCAATTTCGACGACATCAAGGCCGAGGAAGCCGAGGAAGCCAACGACAGCGCCAA
TACCGCAGGCTCCGATGTCGAGGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCGTTCAACATGCGCG
CCTCCGACCTGCACTTCGAGCCCTACGAGCACAACTACCGGGTGCGTTTCCGCGTCGATGGCGAGCTGCGCGAGATCGCC
TCGCCGCCAATTGCGATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGCCTGGACATTTCCGAGAAGCGCGT
GCCGCAAGACGGGCGCATGAAGCTCAAGGTCGGCCCCAACCGCACCATCGACTTCCGCGTGAGCACGCTGCCGACGCTGT
TCGGCGAGAAGATCGTGATCCGTATCCTCGATCCCGAAAGCGCCAAGATGGGCATCGAGGCGCTGGGTTACGAGCCCGAG
GAAAAGGACCGGCTGATGCATGCGATCAGCCGCCCCTACGGCATGATCCTGGTCACCGGCCCCACGGGCTCGGGCAAGAC
CGTTTCGCTCTACACCTGCCTGAACCTGCTCAACAAGCCCGGCGTCAACATCGCCACGGCCGAGGACCCGTCGGAAATCA
ACCTGCCCGGCGTGAACCAGGTCAACGTCAACGAAAAGGCCGGGCTGACCTTCGCCACCGCGCTCAAGGCCTTCCTGCGC
CAGGACCCCGACATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGATACCTCGATCAAGGCCGCGCAGACGGG
CCACCTGGTGCTGTCCACGCTGCATACCAACGACGCGCCCACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGT
TCAACATTGCCTCGAGCGTGATACTGATCACCGCGCAGCGCCTGGCCCGGCGCCTGTGCCCGCAGTGCAAGCAGCCAGCC
GACATCCCGCGCGAGGCGCTGCTCGATGCCGGCTACGACGCCAACGACCTCGATGGCAGCTGGGTCACCTACAAGCCCGT
GGGCTGCAAGGCCTGCAACAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCTCCGAGGAAATCCAGC
GCATCATCCTGCGCGACGGCAGCGCGCTCGAGATCGCGGCCCAGGCCAAGCTCGAAGGCGTGCGTTCGCTGCGCGACTCC
GGCTTGTACAAGGCGCGCCTGGGACTGACTTCGCTGGAAGAAGTGCTGGCCGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(204-471)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N1X7F7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.299

99.139

0.528
  pilB Acinetobacter baumannii D1279779

52.076

99.484

0.518
  pilF Neisseria gonorrhoeae MS11

51.323

97.59

0.501
  pilB Legionella pneumophila strain ERS1305867

48.517

98.623

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.31

97.246

0.441
  pilB Vibrio campbellii strain DS40M4

44.561

98.107

0.437
  pilB Vibrio cholerae strain A1552

45.505

93.804

0.427
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.037

92.427

0.37
  pilF Thermus thermophilus HB27

37.345

97.246

0.363