Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HUF19_RS01745 Genome accession   NZ_CP054475
Coordinates   431225..433288 (+) Length   687 a.a.
NCBI ID   WP_260998225.1    Uniprot ID   -
Organism   Thalassolituus hydrocarboniclasticus strain alknpb1M-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426225..438288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUF19_RS01725 (HUF19_01725) pilM 428361..429458 (+) 1098 WP_366516530.1 pilus assembly protein PilM Machinery gene
  HUF19_RS01730 (HUF19_01730) - 429458..430006 (+) 549 WP_260998222.1 PilN domain-containing protein -
  HUF19_RS01735 (HUF19_01735) pilO 430011..430652 (+) 642 WP_260998223.1 type 4a pilus biogenesis protein PilO -
  HUF19_RS01740 (HUF19_01740) - 430654..431193 (+) 540 WP_260998224.1 pilus assembly protein PilP -
  HUF19_RS01745 (HUF19_01745) pilQ 431225..433288 (+) 2064 WP_260998225.1 type IV pilus secretin PilQ family protein Machinery gene
  HUF19_RS01750 (HUF19_01750) aroK 433292..433807 (+) 516 WP_260998226.1 shikimate kinase AroK -
  HUF19_RS01755 (HUF19_01755) aroB 433813..434904 (+) 1092 WP_260998227.1 3-dehydroquinate synthase -
  HUF19_RS01760 (HUF19_01760) - 434928..436448 (+) 1521 WP_260998228.1 AAA family ATPase -

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 74590.77 Da        Isoelectric Point: 4.5969

>NTDB_id=452703 HUF19_RS01745 WP_260998225.1 431225..433288(+) (pilQ) [Thalassolituus hydrocarboniclasticus strain alknpb1M-1]
MIKALPVLLLALMASAVSAATLNDLKFSSLPGDVTEIRMVFDGTPPQVSGYTIEQPARIALDLPGVVSTLKTKRHQIGSG
NARSATLVSTKDRSRLVVNLTRLVGYSTKVDGNTLLLRIGQTEGEKVFVADNSTDQAGMLSSDIKVVNVDFRRGELGEGQ
VQVMLNDPRASANIRREGNKIIADLKGVRLPEALSRRLDVTDFATPVRFVDTKREDNSTRIVIEPTSDDFEYLAYQTDKL
LSINVSVLPEEQKEERKKQFPFTGEKLSLNFQNIEVRAVLQLIADFTGLNLVASDTVQGSITLRLQNVPWDQALDLVLKT
KGLGKRQMGSVLLIAPAEEIAAREKIELEAVKQVEELAPLVTEYMQLKYAKASELAKLLTSEQGLLSERGSAVVDERTNT
LLMKDTAGNLEKVREALNMLDVPVRQVLIEARIVVATTSVGEEMGVKWGGAGYKNNGNNWTTVGGSQTTIAEGNQVLLDR
FNGDEPEAINLTSANIVDFGVTNAAATTFAVGYQTADYLLDLELAAIETDGRAEIVSQPRVITADGQTASIESGTEIPYQ
QASSSGATNVSFKSAVLRLEVTPQITPDDRIIMDLVINQDSVGELTPAGPSINTNAVETQVLVDNGETVVLGGIFRSEEV
TAISKTPFFGDLPLIGALFRYNSHSEDKSELLVFITPRLVKDSLSNR

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=452703 HUF19_RS01745 WP_260998225.1 431225..433288(+) (pilQ) [Thalassolituus hydrocarboniclasticus strain alknpb1M-1]
ATGATAAAGGCACTACCGGTCCTGCTGCTGGCTTTGATGGCTTCTGCGGTTTCGGCGGCAACGCTGAACGATCTGAAGTT
TTCTTCATTACCCGGAGACGTGACTGAAATCCGCATGGTTTTCGATGGTACGCCGCCGCAGGTCAGTGGCTATACCATTG
AGCAGCCAGCGCGTATCGCTCTGGACTTGCCGGGTGTTGTCAGTACGCTGAAAACAAAGCGCCATCAGATCGGTTCCGGC
AACGCCCGCAGCGCCACTCTGGTGTCGACCAAAGACCGCTCACGTCTGGTTGTTAACCTCACCCGTCTGGTGGGTTACAG
CACCAAGGTTGATGGCAATACCCTGTTACTGCGTATCGGTCAGACAGAAGGCGAGAAAGTATTTGTCGCGGATAACAGCA
CCGATCAGGCAGGCATGCTGAGCAGCGATATCAAAGTGGTTAACGTTGATTTCCGTCGTGGCGAGCTGGGTGAAGGTCAG
GTTCAGGTGATGCTGAACGATCCCCGTGCTTCTGCCAATATCCGTCGCGAAGGCAATAAGATTATTGCCGATCTGAAAGG
CGTTCGTTTACCGGAAGCTCTGAGCCGTCGTCTGGATGTAACCGATTTTGCAACACCGGTACGCTTTGTCGACACCAAGC
GTGAAGATAACTCTACCCGTATCGTGATTGAGCCAACCTCGGACGATTTCGAGTATCTGGCTTATCAGACCGACAAGTTG
CTGTCGATCAACGTTTCTGTATTACCGGAAGAACAGAAAGAAGAACGCAAGAAACAGTTCCCGTTCACCGGCGAGAAGCT
CTCTCTTAACTTCCAGAATATTGAAGTACGTGCGGTTCTGCAGCTGATCGCCGATTTTACCGGCCTCAACCTTGTGGCAT
CCGATACCGTTCAGGGCAGCATTACCCTGCGCCTGCAGAACGTGCCATGGGATCAGGCGCTGGATCTGGTACTGAAAACC
AAAGGTCTGGGTAAACGCCAGATGGGCTCTGTACTGCTGATTGCGCCGGCGGAAGAAATTGCAGCACGTGAAAAAATCGA
GCTGGAAGCGGTTAAACAGGTAGAAGAACTGGCTCCGCTGGTCACCGAATACATGCAGCTGAAATATGCCAAAGCCAGCG
AGCTGGCCAAGCTGCTGACGTCCGAGCAGGGCTTGTTATCTGAGCGTGGTTCGGCGGTTGTCGATGAGCGCACCAATACC
CTGCTGATGAAAGATACCGCCGGTAATCTGGAAAAAGTACGTGAAGCCCTGAATATGCTCGATGTGCCAGTGCGTCAGGT
GCTGATTGAGGCCCGTATCGTCGTTGCCACTACCAGTGTTGGCGAAGAAATGGGTGTTAAGTGGGGTGGTGCCGGCTATA
AAAATAACGGTAATAACTGGACGACGGTTGGCGGCAGTCAGACCACTATTGCGGAAGGTAATCAGGTTCTGCTTGACCGC
TTTAATGGTGACGAGCCGGAAGCCATTAACCTGACTTCAGCAAACATTGTTGATTTCGGTGTGACCAACGCCGCGGCGAC
CACCTTTGCTGTGGGCTATCAGACCGCAGATTACCTGCTGGATCTGGAACTGGCGGCGATTGAAACCGATGGCCGTGCAG
AAATCGTGTCGCAGCCACGGGTTATTACTGCTGATGGCCAGACGGCTTCTATCGAATCCGGTACTGAAATTCCATATCAG
CAGGCCAGCTCCAGCGGTGCTACCAACGTGTCGTTCAAGTCTGCGGTACTGCGTCTGGAAGTAACACCGCAGATTACCCC
GGATGACCGCATTATCATGGATCTGGTAATCAACCAGGATTCCGTGGGCGAACTGACTCCGGCAGGACCAAGCATCAACA
CTAACGCCGTGGAAACTCAGGTTCTGGTCGACAATGGCGAAACGGTGGTGCTGGGTGGTATCTTCCGCTCGGAAGAAGTA
ACGGCAATCAGTAAAACTCCGTTCTTTGGCGACCTGCCATTAATCGGCGCGCTGTTCCGCTATAACAGCCACTCCGAAGA
TAAGAGCGAGCTGCTGGTATTTATCACTCCGCGTCTGGTTAAGGATTCGCTCAGCAATCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(526-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(364-426)

AMIN domain (AMIN)

(35-118)

Secretin and TonB N terminus short domain (STN)

(290-336)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

47.789

100

0.488
  pilQ Acinetobacter baumannii D1279779

41.643

100

0.435
  pilQ Acinetobacter baumannii strain A118

41.341

100

0.431
  comQ Acinetobacter baylyi ADP1

41.051

100

0.421
  pilQ Legionella pneumophila strain ERS1305867

39.617

98.836

0.392
  pilQ Legionella pneumophila strain Lp02

39.617

98.836

0.392