Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HTY51_RS13340 Genome accession   NZ_CP054424
Coordinates   2778011..2779747 (+) Length   578 a.a.
NCBI ID   WP_174253178.1    Uniprot ID   A0A6N0BWE9
Organism   Rhodoferax sp. BAB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2773011..2784747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HTY51_RS13310 (HTY51_13310) proB 2773508..2774617 (-) 1110 WP_371733872.1 glutamate 5-kinase -
  HTY51_RS13315 (HTY51_13315) cgtA 2774772..2775848 (-) 1077 WP_057673746.1 Obg family GTPase CgtA -
  HTY51_RS13320 (HTY51_13320) rpmA 2775989..2776246 (-) 258 WP_057673747.1 50S ribosomal protein L27 -
  HTY51_RS13325 (HTY51_13325) rplU 2776259..2776570 (-) 312 WP_082605348.1 50S ribosomal protein L21 -
  HTY51_RS13330 (HTY51_13330) - 2776781..2777710 (+) 930 WP_174254282.1 polyprenyl synthetase family protein -
  HTY51_RS13340 (HTY51_13340) pilB 2778011..2779747 (+) 1737 WP_174253178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HTY51_RS13345 (HTY51_13345) pilC 2779772..2780989 (+) 1218 WP_174253179.1 type II secretion system F family protein Machinery gene
  HTY51_RS13350 (HTY51_13350) pilD 2780989..2781831 (+) 843 WP_174253180.1 A24 family peptidase Machinery gene
  HTY51_RS13355 (HTY51_13355) coaE 2781835..2782443 (+) 609 WP_174253181.1 dephospho-CoA kinase -
  HTY51_RS13360 (HTY51_13360) zapD 2782466..2783221 (+) 756 WP_174253182.1 cell division protein ZapD -
  HTY51_RS13365 (HTY51_13365) - 2783251..2783469 (+) 219 WP_057673754.1 DNA gyrase inhibitor YacG -
  HTY51_RS13370 (HTY51_13370) - 2783485..2783961 (-) 477 WP_174253183.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62789.29 Da        Isoelectric Point: 6.2117

>NTDB_id=452431 HTY51_RS13340 WP_174253178.1 2778011..2779747(+) (pilB) [Rhodoferax sp. BAB1]
MAAVDTAIHRENAPVALPGLGRALILAGKLEQKSAEELFRKASSNRTSFIAELTGSGAVSAADLAHTMSAAFAAPLIDLD
AVDTQRLPKELLDPKICHDYRVVVLGKRTNRLVVATADPSDQAAAEKIKFASQMGVDWVIAEYDKLLKLVEVHATSASEA
IDNIVGGDIEFDEFTTEAVSDSAESATSEVDDAPVVKFLHKMLIDAIGMRASDLHFEPYEHNYRVRFRIDGELREIASPP
VAIKDKLASRIKVISKMDISEKRVPQDGKMKLKVGPERVIDFRVSTLPTLFGEKIVIRILDPNSAKLGIDALGYEPEEKE
RLLKAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDKAGLTFAAALKSFLRQDP
DIIMVGEIRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPAEIP
KKTMLDAGFQPDELDGSWINYRPVGCSACNNGYKGRVGIYQVMPITEELQRIILRDGSALEIAKQAQAEGVRSLRLSGLH
KVKQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=452431 HTY51_RS13340 WP_174253178.1 2778011..2779747(+) (pilB) [Rhodoferax sp. BAB1]
ATGGCCGCCGTAGATACCGCCATCCACCGCGAAAATGCTCCCGTTGCCCTGCCCGGACTGGGGCGCGCCCTGATTCTGGC
TGGCAAGCTGGAGCAGAAGTCGGCCGAGGAACTGTTCCGCAAGGCGTCCAGCAACCGCACCAGTTTCATCGCCGAACTCA
CGGGTTCGGGAGCCGTTTCTGCTGCGGATCTGGCCCACACCATGTCAGCGGCATTCGCCGCCCCGCTGATCGACCTGGAC
GCCGTCGACACACAACGCCTGCCCAAGGAACTGCTGGACCCCAAGATCTGCCATGACTACCGCGTGGTGGTCCTGGGCAA
GCGCACCAACCGGCTGGTGGTCGCCACGGCCGACCCCTCTGATCAGGCCGCCGCAGAAAAGATCAAGTTTGCCAGCCAGA
TGGGCGTGGACTGGGTCATTGCGGAATATGACAAGCTGCTCAAGCTGGTGGAAGTCCACGCCACCTCGGCCTCGGAAGCC
ATCGACAACATCGTCGGTGGTGATATCGAATTCGACGAGTTCACGACCGAGGCCGTGAGCGACAGCGCCGAGTCCGCCAC
TTCCGAGGTGGACGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATCGATGCCATCGGCATGCGCGCTTCGGACC
TGCATTTCGAGCCCTACGAGCACAACTACCGGGTGCGTTTCCGCATCGACGGCGAACTGCGCGAGATCGCCTCGCCGCCC
GTGGCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGAAGATGGACATCTCCGAGAAGCGGGTGCCGCAAGA
CGGCAAGATGAAGCTCAAGGTCGGCCCCGAACGCGTCATCGATTTCCGGGTCAGCACGCTGCCCACGCTGTTCGGTGAAA
AGATCGTGATCCGTATCCTGGACCCGAACAGCGCCAAGCTGGGCATCGATGCGCTGGGCTACGAACCGGAAGAAAAAGAA
CGGCTGCTCAAGGCCATCAGCCGTCCCTACGGGATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCT
GTACACCTGTCTGAATCTGCTGAACAAGCCGGGGGTGAACATCGCCACGGCCGAGGACCCGTCCGAAATCAACCTGCCGG
GCGTGAACCAGGTCAACGTCAACGACAAGGCCGGCCTGACCTTCGCCGCCGCGCTCAAATCCTTCCTGCGCCAGGATCCC
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGACATCGCCATCAAGGCGGCCCAGACCGGTCACCTGGT
GATGTCCACGCTGCACACCAATGACGCGCCCACCACCCTCACGCGCATGCGCAATATGGGGATCGCCCCGTTCAACATTG
CCTCCAGCGTGATCCTGATCACGGCGCAGCGTCTGGCGCGCCGCCTGTGCCCGAACTGCAAGGCACCGGCCGAGATCCCC
AAGAAGACGATGCTCGATGCGGGCTTCCAGCCGGACGAGCTGGACGGCTCCTGGATCAACTACCGCCCGGTCGGCTGTTC
CGCCTGCAACAACGGCTACAAGGGACGTGTGGGCATCTACCAGGTCATGCCGATCACCGAGGAGCTGCAGCGCATCATCC
TGCGTGATGGCTCCGCCCTGGAAATCGCCAAGCAGGCGCAGGCCGAGGGGGTGCGCTCGCTGCGCCTGTCCGGCCTGCAC
AAGGTCAAGCAGGGCCTGACTTCGCTCGAAGAAGTCCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-151)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0BWE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.615

97.059

0.54
  pilB Acinetobacter baylyi ADP1

54.276

99.135

0.538
  pilF Neisseria gonorrhoeae MS11

52.043

97.405

0.507
  pilB Legionella pneumophila strain ERS1305867

50.355

97.578

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

44.92

97.059

0.436
  pilB Vibrio campbellii strain DS40M4

44.268

98.097

0.434
  pilB Vibrio cholerae strain A1552

46.455

92.734

0.431
  pilF Thermus thermophilus HB27

38.22

99.135

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.262

92.388

0.372