Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQN60_RS03805 Genome accession   NZ_CP054143
Coordinates   794297..796000 (+) Length   567 a.a.
NCBI ID   WP_173532421.1    Uniprot ID   A0A6M8SLF4
Organism   Deefgea piscis strain D17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 789297..801000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN60_RS03775 (HQN60_03775) rplS 789569..789949 (-) 381 WP_173532416.1 50S ribosomal protein L19 -
  HQN60_RS03780 (HQN60_03780) trmD 789994..790755 (-) 762 WP_173532417.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  HQN60_RS03785 (HQN60_03785) rimM 790788..791330 (-) 543 WP_308419424.1 ribosome maturation factor RimM -
  HQN60_RS03790 (HQN60_03790) rpsP 791334..791576 (-) 243 WP_173532418.1 30S ribosomal protein S16 -
  HQN60_RS03795 (HQN60_03795) ffh 791774..793117 (-) 1344 WP_173532419.1 signal recognition particle protein -
  HQN60_RS03800 (HQN60_03800) - 793201..794010 (+) 810 WP_173532420.1 inner membrane protein YpjD -
  HQN60_RS03805 (HQN60_03805) pilB 794297..796000 (+) 1704 WP_173532421.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HQN60_RS03810 (HQN60_03810) pilC 796014..797237 (+) 1224 WP_173532422.1 type II secretion system F family protein Machinery gene
  HQN60_RS03815 (HQN60_03815) pilD 797230..798093 (+) 864 WP_308419425.1 A24 family peptidase Machinery gene
  HQN60_RS03820 (HQN60_03820) coaE 798094..798708 (+) 615 WP_173532423.1 dephospho-CoA kinase -
  HQN60_RS03825 (HQN60_03825) zapD 798727..799524 (+) 798 WP_254456673.1 cell division protein ZapD -
  HQN60_RS03830 (HQN60_03830) - 799521..799712 (+) 192 WP_173532424.1 DNA gyrase inhibitor YacG -
  HQN60_RS03835 (HQN60_03835) - 799795..800553 (+) 759 WP_173532425.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62131.63 Da        Isoelectric Point: 5.2953

>NTDB_id=451104 HQN60_RS03805 WP_173532421.1 794297..796000(+) (pilB) [Deefgea piscis strain D17]
MTAPLISGLARLLVQHGRLIEADAEMLQTTAATNKTSFIEQLIQSKKMSARDIADFSSKTFGYPLLDLDRIDPSYIPQGV
LDSKAMQAQRLVPLYKRGTKLFVGISDITNMAAVENVRFQTNAQVEPIIVEDPKLAVLLNKLLEASGANLKDMVIDEADL
EMAGGDDAPVQDTAANLEIDDAPVVKYLQKILMDAINQGASDIHFEPYEKFFRVRYRVDGVLREVATPPLAIKEKISSRI
KVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTLPTMHGEKIVMRILDPSSATLGIDALGYDPDQKATILEAIHRPYG
MVLVTGPTGSGKTVSLYTCLNLLNKPDINISTAEDPCEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRDL
ETADIAIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIQPFNIASSVILITAQRLARRLCSCKQPVDIPTEALLNAGFDQS
DLDGTWHSYGPNGCEICKDSGYKGRVGIYQVMPITDELNRIIMTNGNAIDIADQARREGVLSLREAGLRKVKQGLTSLEE
IMAVTNE

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=451104 HQN60_RS03805 WP_173532421.1 794297..796000(+) (pilB) [Deefgea piscis strain D17]
ATGACTGCCCCCCTTATTTCTGGGCTCGCACGGCTTTTGGTGCAGCACGGCCGTTTAATCGAAGCTGATGCCGAAATGCT
GCAGACAACCGCAGCCACAAATAAAACCTCGTTCATCGAGCAGTTAATTCAAAGCAAAAAAATGTCGGCGCGAGATATTG
CCGATTTTTCATCAAAGACCTTCGGTTACCCCCTTCTCGATCTTGATCGGATTGATCCTAGCTACATTCCACAAGGCGTA
CTCGATAGCAAGGCCATGCAGGCGCAGCGTCTTGTTCCGCTCTACAAGCGGGGCACTAAATTATTTGTTGGTATCTCTGA
TATTACCAATATGGCGGCAGTGGAAAACGTACGTTTTCAAACCAATGCCCAAGTCGAACCCATCATTGTCGAAGACCCCA
AGCTCGCGGTTTTACTTAATAAACTGCTAGAGGCCAGTGGTGCCAACCTCAAAGATATGGTGATTGATGAGGCCGATTTG
GAAATGGCTGGCGGCGACGATGCGCCAGTTCAAGATACCGCGGCCAATCTTGAAATCGATGACGCGCCTGTTGTTAAGTA
CCTACAAAAAATCTTGATGGATGCCATTAACCAAGGCGCATCGGATATCCACTTTGAACCTTACGAAAAATTTTTCCGCG
TTCGTTATCGTGTCGATGGCGTTTTGCGTGAAGTTGCGACGCCGCCACTGGCGATTAAAGAGAAAATTTCCTCCCGTATC
AAAGTCATTTCCAAACTCGATATTTCAGAAAAACGCGTCCCACAAGATGGTCGGATGAAACTCGTTTTATCCAAAAGCCG
CGCCATTGATTTCCGGGTTTCAACTTTGCCCACCATGCATGGCGAAAAAATCGTTATGCGGATTTTAGATCCATCGTCAG
CGACGCTAGGCATTGATGCTCTTGGCTACGACCCAGATCAAAAAGCGACCATTCTCGAGGCCATTCATCGCCCCTATGGC
ATGGTCTTGGTAACCGGCCCAACTGGCTCAGGTAAAACCGTATCGCTGTATACCTGTTTAAATTTACTCAACAAGCCGGA
TATCAATATTTCTACAGCGGAAGATCCTTGCGAGATTAACTTGCCTGGCATTAACCAAGTTAACGTGAATGATAAAGCCG
GTTTAACATTTTCTGCCGCACTGAAAGCCTTCTTGCGGCAAGATCCTGACATCATCATGGTCGGTGAGATTCGTGATTTA
GAGACTGCGGATATTGCGATTAAAGCCGCCCAAACTGGGCATATGGTTTTTTCTACTCTGCATACCAATGATGCACCAAC
CACGTTAACGCGGATGTTAAATATGGGTATTCAACCCTTTAACATCGCCTCTTCGGTAATCTTGATTACCGCGCAACGTT
TGGCGCGTCGACTGTGTAGCTGCAAGCAGCCAGTTGATATACCCACCGAGGCCCTACTTAATGCTGGTTTTGATCAAAGT
GATCTTGATGGAACATGGCATAGTTACGGCCCTAATGGCTGTGAAATATGCAAAGACAGCGGCTATAAGGGCCGAGTTGG
CATTTATCAAGTGATGCCCATTACTGACGAACTCAATCGCATTATTATGACCAACGGCAATGCCATTGATATTGCCGACC
AAGCACGCCGTGAAGGGGTTCTCTCTTTACGTGAAGCGGGCTTAAGAAAAGTAAAACAAGGTCTCACTTCTTTAGAAGAA
ATTATGGCCGTTACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8SLF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.559
  pilB Acinetobacter baylyi ADP1

55.536

100

0.557
  pilF Neisseria gonorrhoeae MS11

54.352

99.295

0.54
  pilB Legionella pneumophila strain ERS1305867

53.004

99.824

0.529
  pilB Vibrio parahaemolyticus RIMD 2210633

46.277

99.471

0.46
  pilB Vibrio cholerae strain A1552

45.775

100

0.459
  pilB Vibrio campbellii strain DS40M4

44.7

99.824

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.777

93.298

0.39
  pilF Thermus thermophilus HB27

37.719

100

0.379