Detailed information NTG0451049

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HRJ47_RS14840
• Genome accession: NZ_CP054137
• Coordinates: 3042572..3043690 (+)
• Length: 372 a.a.
• NCBI ID: WP_086400212.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain JXA13
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3037572..3048690

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HRJ47_RS14830 (HRJ47_14820)	yggS	3040696..3041388 (-)	693	WP_044099923.1	YggS family pyridoxal phosphate-dependent enzyme	-
HRJ47_RS14835 (HRJ47_14825)	pilT	3041515..3042552 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
HRJ47_RS14840 (HRJ47_14830)	pilU	3042572..3043690 (+)	1119	WP_086400212.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HRJ47_RS14845 (HRJ47_14835)	fur	3043760..3044197 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
HRJ47_RS14850 (HRJ47_14840)	omlA	3044309..3044707 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
HRJ47_RS14855 (HRJ47_14845)	-	3044772..3045086 (-)	315	WP_014205982.1	RnfH family protein	-
HRJ47_RS14860 (HRJ47_14850)	-	3045086..3046153 (-)	1068	WP_140157923.1	hypothetical protein	-
HRJ47_RS14865 (HRJ47_14855)	-	3046307..3046762 (-)	456	WP_086400214.1	bacteriohemerythrin	-
HRJ47_RS14870 (HRJ47_14860)	-	3047014..3047862 (-)	849	WP_086400240.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41935.14 Da
• Isoelectric Point: 6.6404

>NTDB_id=451049 HRJ47_RS14840 WP_086400212.1 3042572..3043690(+) (pilU) [Acinetobacter pittii strain JXA13]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGSEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=451049 HRJ47_RS14840 WP_086400212.1 3042572..3043690(+) (pilU) [Acinetobacter pittii strain JXA13]
ATGGATTTTAATGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTACTAAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAATAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCACGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTTCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	97.849	0.403
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363