Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   I6G22_RS09075 Genome accession   NZ_CP065737
Coordinates   1674402..1675043 (-) Length   213 a.a.
NCBI ID   WP_003131746.1    Uniprot ID   Q9CI22
Organism   Lactococcus lactis strain FDAARGOS_865     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1669402..1680043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G22_RS09055 (I6G22_09060) cysK 1669433..1670365 (-) 933 WP_003131750.1 cysteine synthase A -
  I6G22_RS09060 (I6G22_09065) - 1670583..1671668 (+) 1086 WP_023188936.1 LysM peptidoglycan-binding domain-containing protein -
  I6G22_RS09065 (I6G22_09070) - 1671707..1672339 (-) 633 WP_003131748.1 MBL fold metallo-hydrolase -
  I6G22_RS09070 (I6G22_09075) - 1672340..1674334 (-) 1995 WP_025016908.1 transglycosylase domain-containing protein -
  I6G22_RS09075 (I6G22_09080) recU 1674402..1675043 (-) 642 WP_003131746.1 Holliday junction resolvase RecU Machinery gene
  I6G22_RS09080 (I6G22_09085) - 1675124..1675648 (+) 525 WP_023188934.1 DUF1273 domain-containing protein -
  I6G22_RS09085 (I6G22_09090) spx 1675808..1676194 (+) 387 WP_010905435.1 transcriptional regulator Spx -
  I6G22_RS09090 (I6G22_09095) - 1676286..1676567 (+) 282 WP_003131743.1 UPF0223 family protein -
  I6G22_RS09095 (I6G22_09100) - 1676533..1677312 (+) 780 WP_032943467.1 inositol monophosphatase family protein -
  I6G22_RS09100 (I6G22_09105) murA 1677406..1678692 (+) 1287 WP_025016910.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  I6G22_RS09105 (I6G22_09110) - 1678692..1678883 (+) 192 WP_003131739.1 DNA-directed RNA polymerase subunit beta -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 24369.13 Da        Isoelectric Point: 10.1344

>NTDB_id=451048 I6G22_RS09075 WP_003131746.1 1674402..1675043(-) (recU) [Lactococcus lactis strain FDAARGOS_865]
MVNYPNGRSRAYSTVPKKKKSLTELSVTKKSTSKTKGMVAFGKRGMNFEAEINATNEYYLSRGLAVIHKKPTPIQIVKVD
YPQRSRAKITEAYFRQASTTDYSGVYKGHYIDFEAKETQQKTVFPLKNFHEHQIVHMSNVLAQGGIAFVLLHFARLNETY
LLPSSCLITFYYEKGGLKSIPLSHIRENGYKIETNHIPRIPYLEIVNKLCEVQ

Nucleotide


Download         Length: 642 bp        

>NTDB_id=451048 I6G22_RS09075 WP_003131746.1 1674402..1675043(-) (recU) [Lactococcus lactis strain FDAARGOS_865]
ATGGTTAATTATCCTAATGGAAGGTCGCGAGCTTACAGCACTGTTCCTAAGAAAAAAAAATCACTGACAGAGCTGTCAGT
AACTAAAAAAAGCACTTCAAAAACCAAAGGGATGGTTGCCTTCGGGAAGCGGGGAATGAATTTTGAAGCTGAAATTAATG
CAACAAATGAGTATTATCTTTCTCGTGGACTTGCAGTTATCCACAAAAAGCCAACACCAATTCAAATCGTTAAAGTAGAT
TATCCACAAAGGAGTCGTGCAAAAATTACAGAAGCTTACTTCAGGCAAGCATCTACTACTGACTACTCTGGAGTCTATAA
AGGTCACTACATCGACTTTGAAGCAAAGGAAACACAGCAAAAAACTGTTTTCCCTCTAAAAAATTTCCATGAGCATCAAA
TTGTTCACATGAGTAATGTTCTCGCTCAAGGGGGAATTGCTTTTGTCCTCTTGCACTTTGCAAGGCTAAATGAAACATAT
CTTCTTCCATCTTCGTGTTTGATTACTTTTTATTACGAAAAAGGCGGTTTAAAGTCAATTCCGCTTTCTCATATTCGCGA
AAATGGCTATAAAATTGAAACGAACCACATTCCTAGAATTCCTTATCTAGAAATTGTGAATAAACTTTGTGAGGTACAGT
AA

Domains

Predicted by InterProScan.

Recombination protein U (RecU)

(44-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CI22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

47.393

99.061

0.469