Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HQ912_RS26360 Genome accession   NZ_CP054128
Coordinates   5941374..5943446 (-) Length   690 a.a.
NCBI ID   WP_047225964.1    Uniprot ID   -
Organism   Pseudomonas sp. MPDS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5936374..5948446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ912_RS26345 (HQ912_26345) - 5938030..5939592 (-) 1563 WP_173407468.1 AAA family ATPase -
  HQ912_RS26350 (HQ912_26350) aroB 5939603..5940703 (-) 1101 WP_047225963.1 3-dehydroquinate synthase -
  HQ912_RS26355 (HQ912_26355) aroK 5940851..5941369 (-) 519 WP_003196983.1 shikimate kinase AroK -
  HQ912_RS26360 (HQ912_26360) pilQ 5941374..5943446 (-) 2073 WP_047225964.1 type IV pilus secretin PilQ Machinery gene
  HQ912_RS26365 (HQ912_26365) - 5943443..5943988 (-) 546 WP_173407469.1 pilus assembly protein PilP -
  HQ912_RS26370 (HQ912_26370) pilO 5943985..5944608 (-) 624 WP_047225966.1 type 4a pilus biogenesis protein PilO -
  HQ912_RS26375 (HQ912_26375) - 5944605..5945171 (-) 567 WP_047225967.1 PilN domain-containing protein -
  HQ912_RS26380 (HQ912_26380) comM 5945171..5946235 (-) 1065 WP_173407470.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74611.80 Da        Isoelectric Point: 5.4013

>NTDB_id=450934 HQ912_RS26360 WP_047225964.1 5941374..5943446(-) (pilQ) [Pseudomonas sp. MPDS]
MNRFFSALGISLWIALLSPMVHAVNLKTLDVAALPGDRIELKLTFDGPPPAPRGYTTEQPARIALDLPGVTSQLAHKRHD
LGAGNARSATVVEAADRTRLIIGLTQLAPYTARVEGNSLLVVVGKGAVASATAQAPRPAAPSAVPTRASAPVGKAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFAKTRLPEPLRVRLDVKDFATPVQFVNASAGADRATISIEPSGTFD
YSTYQTDNKLTVSVRPMTVEDLQKRDASREAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVTTGD
STTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEQKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=450934 HQ912_RS26360 WP_047225964.1 5941374..5943446(-) (pilQ) [Pseudomonas sp. MPDS]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGCGGTCAATCTGAA
AACGCTGGATGTCGCGGCGCTGCCGGGGGACCGCATCGAGTTGAAACTCACCTTCGACGGGCCGCCCCCTGCGCCTCGGG
GCTACACGACCGAGCAGCCGGCCCGGATCGCTCTGGATCTGCCGGGGGTGACCAGCCAGTTGGCCCACAAGAGACATGAT
CTTGGTGCTGGCAATGCGCGAAGCGCCACGGTGGTCGAGGCGGCTGATCGTACCCGTCTGATCATTGGCCTTACCCAGCT
TGCGCCCTATACCGCGCGGGTGGAGGGCAACAGCCTGCTCGTGGTGGTCGGCAAGGGAGCGGTTGCCAGCGCTACTGCCC
AGGCGCCACGCCCGGCGGCTCCCAGCGCTGTGCCCACCAGAGCCAGTGCGCCGGTGGGCAAGGCGATCCGAGGGGTAGAT
TTCCAGCGCGGCACCCAGGGCGAAGGCAACGTGGTGATCGATCTGTCGGACCCCTCGATCGCCCCGGACATCCAGGAGCG
TGAAGGCAAGATCATCCTCAACTTCGCCAAGACCCGGTTACCCGAACCGCTGCGCGTGCGTCTGGACGTCAAGGACTTCG
CCACCCCGGTGCAGTTCGTCAATGCCAGTGCCGGCGCCGACCGGGCCACCATCAGCATCGAACCCAGCGGTACTTTCGAT
TACTCCACCTACCAGACGGACAACAAACTGACCGTCAGTGTCCGACCCATGACTGTCGAGGATCTGCAAAAACGCGATGC
CAGTCGCGAGGCCTACAGCGGTGAAAAGCTGTCCCTCAACTTTCAGGACATCGAGGTGCGCTCGGTCCTGCAACTGATTG
CCGATTTCACCAACCTCAACCTGGTGGCCAGTGACACGGTGCAGGGCGGCATTACCCTGCGCCTGCAAAATGTGCCTTGG
GACCAGGCGCTGGACCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGTCGGCAATGTGCTGCTGGTCGCCCCCGC
CGACGAAATCGCGGCCCGTGAGCGCCAGGAACTCGAATCCCAGAAGCAGATTGCCGACCTGGCGCCGCTACGGCGTGAGC
TGTTGCAGGTCAACTATGCGAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAAGCCAAGGCCGAT
GAGCGCGGTACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAACTGCG
CCGGATCGTGGCGCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACGTTGACTACG
ACAAGAGCCTGGGCGTGCGCTGGGGTGGATCGATCCAGAACAAAGGCAACTGGAACACTTCAGGGGTCACCACCGGCGAC
TCCACCACCATCGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTTGTCGATCTGGGCGCGTCGGGCAATACCTCCGG
CATCGGCATCGCCTTCATCACCGACAATGTACTGCTGGACCTGGAATTGACGGCCATGGAGAAAACCGGCAACGGGGAAA
TCGTCTCTCAGCCCAAGGTGGTGACGTCCGACAAAGAGACGGCAAAAATCCTCAAGGGCACGGAGATTCCCTATCAGGAA
GCCAGTTCCAGTGGTGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCTTGGAGGTGACCCCGCAGATCACCCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAGGTGAATGATGTGCCGCCAATCA
AGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAG
AGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GCAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.448

100

0.751
  pilQ Acinetobacter baumannii strain A118

38.784

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.784

100

0.416
  comQ Acinetobacter baylyi ADP1

41.245

100

0.413
  pilQ Legionella pneumophila strain Lp02

37.926

97.826

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.926

97.826

0.371