Detailed information NTG0450023

Bioinformatically predicted

Overview

• Name: rarA
• Type: Machinery gene
• Locus tag: I5367_RS12500
• Genome accession: NZ_CP065539
• Coordinates: 2579732..2581000 (+)
• Length: 422 a.a.
• NCBI ID: WP_012118079.1
• Uniprot ID: A7Z748
• Organism: Bacillus amyloliquefaciens strain DGL1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2574732..2586000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5367_RS12480 (I5367_12480)	-	2576103..2576723 (-)	621	WP_088461189.1	tetratricopeptide repeat protein	-
I5367_RS12485 (I5367_12485)	mnmA	2576811..2577926 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
I5367_RS12490 (I5367_12490)	-	2577963..2579102 (-)	1140	WP_012118078.1	cysteine desulfurase family protein	-
I5367_RS12495 (I5367_12495)	cymR	2579121..2579537 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
I5367_RS12500 (I5367_12500)	rarA	2579732..2581000 (+)	1269	WP_012118079.1	replication-associated recombination protein A	Machinery gene
I5367_RS12505 (I5367_12505)	-	2581117..2581881 (-)	765	WP_012118080.1	ThiF family adenylyltransferase	-
I5367_RS12515 (I5367_12515)	aspS	2582207..2583985 (-)	1779	WP_012118081.1	aspartate--tRNA ligase	-
I5367_RS12520 (I5367_12520)	hisS	2584000..2585274 (-)	1275	WP_012118082.1	histidine--tRNA ligase	-
I5367_RS12525 (I5367_12525)	-	2585631..2585801 (-)	171	WP_012118083.1	YrzK family protein	-

Sequence

Protein

• Length: 422 a.a.
• Molecular weight: 46675.54 Da
• Isoelectric Point: 7.9149

>NTDB_id=450023 I5367_RS12500 WP_012118079.1 2579732..2581000(+) (rarA) [Bacillus amyloliquefaciens strain DGL1]
MKPLAYRMRPANIEDIIGQEHLVKEDKIIGRMVRAKHLSSMILYGPPGIGKTSIATAIAGSTSIAFRKLNAVIHNKKDME
IVVQEAKMSGQVILILDEVHRLDKGKQDFLLPYLENGMIILIGATTANPYHAINPAIRSRTQIFELEPLTPDLIKQALER
ALTDEHRGLGSYSVSVDDDAMDHFAQGCGGDVRSALNALELAVLSTKESSDGTIRITRETAEECLQKKSYTHDKNGDAHY
DVLSAFQKSIRGSDANAALHYLARLIEAGDLESISRRLLVIAYEDIGLASPQAGPRVLSAIQTAERIGFPEARIPLANAV
IELCLSPKSNSAISAVDEALKDIRTGKIGDVPKHLKDAHYKGAQELGRGIGYQYPHDFENGWVEQQYLPEPLKNKQYYKP
KQTGKFEAAIKQVYEKLMKQKK

Nucleotide

• Length: 1269 bp

>NTDB_id=450023 I5367_RS12500 WP_012118079.1 2579732..2581000(+) (rarA) [Bacillus amyloliquefaciens strain DGL1]
ATGAAACCATTGGCGTATCGGATGCGGCCTGCCAATATCGAAGACATTATCGGCCAGGAACATTTGGTCAAAGAGGATAA
AATCATCGGCAGAATGGTGCGTGCAAAGCACTTATCATCCATGATCCTGTACGGACCGCCGGGCATCGGAAAAACGTCCA
TCGCCACAGCGATCGCAGGATCGACAAGCATCGCATTCAGGAAATTGAATGCCGTCATTCATAATAAAAAAGACATGGAG
ATTGTCGTACAGGAAGCGAAAATGTCAGGCCAGGTCATTTTAATCCTTGACGAAGTGCACAGGCTTGATAAGGGAAAGCA
GGATTTTCTGCTGCCCTATTTAGAAAACGGCATGATTATTTTAATCGGCGCGACGACCGCCAACCCTTATCATGCCATTA
ACCCGGCCATCAGAAGCCGGACCCAGATATTTGAGCTTGAACCGTTAACACCCGATCTGATCAAGCAGGCATTGGAAAGA
GCGCTCACAGATGAACACCGGGGGCTCGGTTCCTACAGCGTTTCGGTGGACGACGACGCGATGGATCATTTCGCGCAAGG
GTGCGGAGGAGACGTCCGCTCAGCTTTGAACGCACTTGAGCTTGCCGTCCTCTCCACAAAGGAATCAAGCGACGGCACGA
TCCGGATTACACGGGAGACGGCGGAAGAATGTCTGCAGAAAAAAAGCTATACTCATGATAAAAACGGTGATGCGCATTAT
GACGTCTTATCCGCCTTCCAAAAATCGATCCGAGGCTCGGACGCAAACGCCGCCCTTCATTATCTGGCCCGTCTGATCGA
AGCCGGCGACCTTGAAAGCATATCAAGAAGGCTTCTCGTGATCGCCTATGAAGATATCGGCCTCGCAAGCCCGCAGGCAG
GCCCCCGGGTGCTGAGCGCGATTCAGACCGCAGAACGGATCGGCTTTCCGGAAGCGAGAATTCCGCTGGCCAATGCGGTA
ATCGAATTATGCCTCTCGCCAAAATCAAACTCCGCCATCTCAGCCGTTGATGAGGCGCTCAAAGACATCAGGACAGGTAA
AATCGGCGATGTGCCGAAACATTTAAAAGACGCCCACTATAAAGGGGCCCAAGAGCTCGGACGCGGGATCGGCTATCAAT
ATCCTCACGATTTCGAGAACGGATGGGTCGAACAGCAATATTTGCCTGAACCGTTAAAAAACAAGCAATACTATAAACCG
AAACAAACGGGGAAATTTGAAGCCGCCATAAAGCAAGTGTATGAAAAACTGATGAAGCAGAAAAAATAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (8-125)

• MgsA AAA+ ATPase C terminal (MgsA_C) (252-417)

• AAA C-terminal domain (AAA_assoc_2) (172-251)

3D structure

• AlphaFold DB: A7Z748

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rarA	Bacillus subtilis subsp. subtilis str. 168	90.974	99.763	0.908