Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FOC66_RS10145 Genome accession   NZ_CP053939
Coordinates   2112231..2113910 (-) Length   559 a.a.
NCBI ID   WP_003748316.1    Uniprot ID   -
Organism   Neisseria mucosa strain FDAARGOS_758     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2107231..2118910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC66_RS10115 (FOC66_10115) lspA 2107586..2108092 (-) 507 WP_003748308.1 signal peptidase II -
  FOC66_RS10120 (FOC66_10120) glyQ 2108241..2109140 (-) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  FOC66_RS10125 (FOC66_10125) yacG 2109222..2109404 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  FOC66_RS10130 (FOC66_10130) coaE 2109401..2110021 (-) 621 WP_003748310.1 dephospho-CoA kinase -
  FOC66_RS10135 (FOC66_10135) pilD 2110021..2110908 (-) 888 WP_003748312.1 A24 family peptidase Machinery gene
  FOC66_RS10140 (FOC66_10140) pilG 2110910..2112151 (-) 1242 WP_003748314.1 type II secretion system F family protein Machinery gene
  FOC66_RS10145 (FOC66_10145) pilF 2112231..2113910 (-) 1680 WP_003748316.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOC66_RS10150 (FOC66_10150) carB 2114314..2117529 (-) 3216 WP_003748319.1 carbamoyl-phosphate synthase large subunit -
  FOC66_RS10155 (FOC66_10155) - 2117558..2118463 (-) 906 WP_003748321.1 hypothetical protein -
  FOC66_RS10160 (FOC66_10160) - 2118585..2118755 (+) 171 WP_161800351.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62311.52 Da        Isoelectric Point: 5.8686

>NTDB_id=449276 FOC66_RS10145 WP_003748316.1 2112231..2113910(-) (pilF) [Neisseria mucosa strain FDAARGOS_758]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPDLFSDGIITPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCVPIFKRGRKVYFAVSDPTQIQNFQKIAFSSGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINEEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFATALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRIGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=449276 FOC66_RS10145 WP_003748316.1 2112231..2113910(-) (pilF) [Neisseria mucosa strain FDAARGOS_758]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCGACTTGTTTTCAGACGGCATCATCACCCCTAAAGCCTTGGGCGAATTGGTTGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTACACTACTATCCGCGCAATAATGTAGTCAGCGACATCCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCGTTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACTCAAATCCA
AAACTTCCAAAAAATCGCTTTTTCTTCAGGTTTAAGTGTTGATTTGGTAGTGGTTCCCGATGACCAACTCAGCTCCTTAC
TGGAATGGTTGGGTCAGCGTTCGACCACCATTCTGAAAGAAATCAACGAAGAACATGAAGCAACGCAGCAATCTCAGTCG
CTTTATATCGATAATGAAGAGGCTGAAGACGGCCCTATCCCCCGCTTCATCCACAAAACCTTATCCGATGCCCTAAACGC
AGGTGCATCAGATATCCACTTTGAATTCTACGAACAAATGGCGCGCGTCCGTTTCCGCGTGGACGGACAGTTGCGCGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGTCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCTGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCACAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACGCTGTTTGGCGAAAAAGTAGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGCCCTTACGGCATGGTGTTGGTTACCGGCCCAACAGGCTCG
GGTAAAACCGTTTCGCTCTATACCTGCCTGAGCATTTTGAACACCGAAGATGTCAACATTTCAACGGCAGAGGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAGAAACAAGGCCTGACGTTTGCCACCGCACTCCGATCCT
TCTTGCGTCAAGATCCTGATATTATTATGGTCGGTGAGATCCGCGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCGACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
GGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGGCTGTGTCCAAACTGCAAAC
GCGAAGTGGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAGCTC
TACCGCCCGGTTGGTTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCATCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACGGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACCTGCGTCGCGCCGGCCTCATGAAAGTCATGCAGGGCCTGACTTCGTTGGAAGAAGTAACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.102

100

0.771
  pilB Acinetobacter baylyi ADP1

50.35

100

0.515
  pilB Acinetobacter baumannii D1279779

50.088

100

0.506
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

45.73

100

0.46
  pilB Vibrio campbellii strain DS40M4

44.425

100

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.447

96.78

0.372