Detailed information NTG0449170

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FOC88_RS20415
• Genome accession: NZ_CP053934
• Coordinates: 3846189..3849914 (-)
• Length: 1241 a.a.
• NCBI ID: WP_042511618.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain FDAARGOS_794
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3841189..3854914

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC88_RS20365 (FOC88_20360)	-	3842123..3842407 (+)	285	WP_000925338.1	hypothetical protein	-
FOC88_RS20370 (FOC88_20365)	-	3842443..3843342 (-)	900	WP_042511615.1	fumarylacetoacetate hydrolase family protein	-
FOC88_RS20375 (FOC88_20370)	-	3843590..3843769 (+)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
FOC88_RS20380 (FOC88_20375)	gerPA	3843866..3844087 (+)	222	WP_001111188.1	spore germination protein GerPA	-
FOC88_RS20385 (FOC88_20380)	gerPB	3844102..3844308 (+)	207	WP_001012512.1	spore germination protein GerPB	-
FOC88_RS20390 (FOC88_20385)	gerPC	3844376..3844990 (+)	615	WP_001070767.1	spore germination protein GerPC	-
FOC88_RS20395 (FOC88_20390)	gerPD	3844997..3845191 (+)	195	WP_001052802.1	spore germination protein GerPD	-
FOC88_RS20400 (FOC88_20395)	-	3845207..3845593 (+)	387	WP_042511616.1	spore germination protein GerPE	-
FOC88_RS20405 (FOC88_20400)	gerPF	3845636..3845851 (+)	216	WP_001141566.1	spore germination protein GerPF	-
FOC88_RS20410 (FOC88_20405)	-	3845889..3846176 (-)	288	WP_042511617.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
FOC88_RS20415 (FOC88_20410)	addA	3846189..3849914 (-)	3726	WP_042511618.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FOC88_RS20420 (FOC88_20415)	addB	3849911..3853426 (-)	3516	WP_042511619.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FOC88_RS20425 (FOC88_20420)	lepB	3853543..3854106 (-)	564	WP_000751894.1	signal peptidase I	-
FOC88_RS20430 (FOC88_20425)	-	3854163..3854756 (-)	594	WP_000347516.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142723.10 Da
• Isoelectric Point: 4.8339

>NTDB_id=449170 FOC88_RS20415 WP_042511618.1 3846189..3849914(-) (addA) [Bacillus thuringiensis strain FDAARGOS_794]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNMIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSAAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLCRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
ETRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=449170 FOC88_RS20415 WP_042511618.1 3846189..3849914(-) (addA) [Bacillus thuringiensis strain FDAARGOS_794]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGAGAGGCTTTAGAAAAAGTATTAATTGATGAGCCTGGCTCTCAGCACATCCGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACCGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATATGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCAGCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAACAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTAAAATA
CCGTGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTCGCTGGACTTTGTCGTCGTTTTAATACGCAAGACTTAATGAA
ACGTTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGGTTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCGGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTA
GAAACTCGTTACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGTTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.586	100	0.536