Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HP062_RS15825 Genome accession   NZ_CP053929
Coordinates   3465112..3466812 (+) Length   566 a.a.
NCBI ID   WP_172791654.1    Uniprot ID   -
Organism   Pseudomonas sp. B14-6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3460112..3471812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP062_RS15805 (HP062_15805) - 3460278..3463232 (+) 2955 WP_172791652.1 DUF748 domain-containing protein -
  HP062_RS15810 (HP062_15810) - 3463232..3463528 (+) 297 WP_154501766.1 DUF2845 domain-containing protein -
  HP062_RS15815 (HP062_15815) - 3463814..3464167 (-) 354 WP_010467586.1 BON domain-containing protein -
  HP062_RS15820 (HP062_15820) pilA 3464479..3464886 (-) 408 WP_172791653.1 pilin Machinery gene
  HP062_RS15825 (HP062_15825) pilB 3465112..3466812 (+) 1701 WP_172791654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HP062_RS15830 (HP062_15830) pilC 3466815..3468032 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  HP062_RS15835 (HP062_15835) pilD 3468034..3468906 (+) 873 WP_172791655.1 A24 family peptidase Machinery gene
  HP062_RS15840 (HP062_15840) coaE 3468903..3469526 (+) 624 WP_042932554.1 dephospho-CoA kinase -
  HP062_RS15845 (HP062_15845) yacG 3469523..3469723 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  HP062_RS15850 (HP062_15850) - 3469727..3469942 (-) 216 WP_172791656.1 hypothetical protein -
  HP062_RS15855 (HP062_15855) - 3470006..3470695 (-) 690 WP_083375730.1 energy-coupling factor ABC transporter permease -
  HP062_RS15860 (HP062_15860) - 3470692..3471159 (-) 468 WP_172791657.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62629.83 Da        Isoelectric Point: 5.8850

>NTDB_id=449071 HP062_RS15825 WP_172791654.1 3465112..3466812(+) (pilB) [Pseudomonas sp. B14-6]
MNDIALSGLAKQLVMAELLTDQSAQQAYLQAQRSKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKEAQPRGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLGDAIEKFFDNQATGLEDMADVDLDG
LDIESVDDNKKDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPSQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKALYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIQGFNIATSVTLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=449071 HP062_RS15825 WP_172791654.1 3465112..3466812(+) (pilB) [Pseudomonas sp. B14-6]
ATGAATGACATCGCCCTAAGCGGTCTGGCCAAGCAACTGGTCATGGCCGAACTGCTCACTGACCAAAGCGCGCAACAGGC
GTATCTGCAAGCCCAACGCAGCAAAATCTCCCTGGTCAGTTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGGCAGGTTG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTCGACCTCAATTGCCTGGACAAGGAAGCCCAGCCTCGAGGGTTG
GTCAGCGAGAAACTGATCCGTCAGCACCACGCCCTGCCCCTCTGGCGCCGTGGCAACAAACTGTTCGTGGGGGTTTCCGA
CCCGACCAATCATCAAGCCATCAATGACATCCAGTTCAGCACCGGTCTGAACACCGAAGCCATTCTGGTAGAGGACGATA
AGCTCGGCGATGCCATCGAGAAGTTCTTCGACAACCAAGCAACCGGCCTGGAAGACATGGCCGATGTTGACCTTGACGGG
CTGGACATCGAGTCGGTCGACGACAACAAAAAGGACGCCATCGCCGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGGG
TGCGGATGCGCACCGACGGCATGCTGCGAGAAGTCGCCAAGCCGCCGAGCCAACTGGCCAACCGCATTGCGGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCCAAGACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGCCTGAACAGAAAGCCTTGTACATGGCTGCACTGAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCGACCGGTTCGGGCAAGACCGTGTCGCTCTACACCGGGTTGAATATCCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTCGCCCAGGCTCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGACCTC
GAGACCGCCGAAATCGCCATCAAGGCTGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGCTTCAACATCGCCACCTCGGTCACCCTGATCATCGCCCAGCGGC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGGCGTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGATCAGTGCAATGGCGGTTACAAGGGGCGAGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGACATTCAGA
TGCGCAAGGACGGCTTTAATGACTTGCGCACCTCGGGCTTGATCAAGGCCATGCAAGGCGTCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.398

99.823

0.553
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.117

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.463

0.366