Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HP062_RS15830 Genome accession   NZ_CP053929
Coordinates   3466815..3468032 (+) Length   405 a.a.
NCBI ID   WP_010467583.1    Uniprot ID   A0A024EER5
Organism   Pseudomonas sp. B14-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3461815..3473032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP062_RS15810 (HP062_15810) - 3463232..3463528 (+) 297 WP_154501766.1 DUF2845 domain-containing protein -
  HP062_RS15815 (HP062_15815) - 3463814..3464167 (-) 354 WP_010467586.1 BON domain-containing protein -
  HP062_RS15820 (HP062_15820) pilA 3464479..3464886 (-) 408 WP_172791653.1 pilin Machinery gene
  HP062_RS15825 (HP062_15825) pilB 3465112..3466812 (+) 1701 WP_172791654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HP062_RS15830 (HP062_15830) pilC 3466815..3468032 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  HP062_RS15835 (HP062_15835) pilD 3468034..3468906 (+) 873 WP_172791655.1 A24 family peptidase Machinery gene
  HP062_RS15840 (HP062_15840) coaE 3468903..3469526 (+) 624 WP_042932554.1 dephospho-CoA kinase -
  HP062_RS15845 (HP062_15845) yacG 3469523..3469723 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  HP062_RS15850 (HP062_15850) - 3469727..3469942 (-) 216 WP_172791656.1 hypothetical protein -
  HP062_RS15855 (HP062_15855) - 3470006..3470695 (-) 690 WP_083375730.1 energy-coupling factor ABC transporter permease -
  HP062_RS15860 (HP062_15860) - 3470692..3471159 (-) 468 WP_172791657.1 FAD/FMN-containing dehydrogenase -
  HP062_RS15865 (HP062_15865) - 3471288..3471914 (+) 627 WP_007970534.1 DUF1780 domain-containing protein -
  HP062_RS15870 (HP062_15870) - 3471911..3472438 (+) 528 WP_010467568.1 MOSC domain-containing protein -
  HP062_RS15875 (HP062_15875) - 3472490..3472663 (+) 174 WP_008028730.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44190.99 Da        Isoelectric Point: 9.7373

>NTDB_id=449072 HP062_RS15830 WP_010467583.1 3466815..3468032(+) (pilC) [Pseudomonas sp. B14-6]
MAVKAAKISVYAWEGTDRKGSKMTGELSGLNPALIKAQLRKQGINPGKVRKKSASIFSFGNRIKAQEIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLIKVVPQFESVFKGFGAELPAFTVMVIGLSEFLQAWWWMMLGALIAAIFGVR
HAFKTSEAFRDRTDTWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPSMAIQLTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=449072 HP062_RS15830 WP_010467583.1 3466815..3468032(+) (pilC) [Pseudomonas sp. B14-6]
ATGGCGGTCAAAGCAGCAAAAATCAGCGTCTACGCCTGGGAAGGCACGGACAGGAAAGGATCAAAGATGACCGGCGAGTT
GAGCGGTCTCAACCCGGCCTTGATCAAGGCTCAGTTGCGCAAGCAGGGGATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CCTCGATTTTCAGTTTCGGCAATCGGATCAAGGCTCAGGAAATCGCCCTGTTTACCCGGCAAATGGCAACGATGATGAAA
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATCGGTGAAGGCTTCGACAACCCGGCCATGCGCAAACTGGTGGACGA
GGTGAAGCAGGAAGTCGCGGCGGGTAACAGCTTCGCCGCGTCCTTGCGTAAAAAACCGCAGTATTTCGACGAGCTGTACT
GCAACCTCGTCGACGCAGGTGAGCAGGCTGGCGCTCTGGATACTCTGCTGGAACGGGTGGCGACCTATAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCCGTGGTCTTTGTGGCGATCATTGTCACCGGGAT
TCTGCTGATCAAGGTGGTGCCGCAGTTCGAGTCGGTGTTCAAGGGCTTTGGCGCCGAGTTGCCGGCCTTCACTGTGATGG
TCATCGGCTTGTCGGAGTTCTTGCAGGCCTGGTGGTGGATGATGCTTGGCGCGTTGATCGCCGCGATCTTCGGTGTGCGC
CACGCCTTTAAAACATCCGAGGCCTTTCGGGACCGCACGGACACCTGGCTGCTGAAGCTGCCGCTGATCGGCACCTTGAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCCTGTCGACCACATTCGCCGCCGGCGTGCCGTTGGTCGAAGCCCTGG
ATTCAGTGGCGGGCGCGACCGGCAACATCGTGTTCAAGCGGGCGGTGCTGCGCATCAAGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTCTCGATGCGCACCTCAGGCATCTTTCCCAGTATGGCGATCCAGCTGACCGCCATTGGCGAAGAATCCGG
CGCTCTGGACGACATGCTCGATAAGGTCGCGAGCTTCTACGAGGACGAAGTGGACAATATGGTCGATAACCTCACCAGCT
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATTGTCGGTGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EER5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

58.333

100

0.588
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

40.148

100

0.402
  pilC Thermus thermophilus HB27

37.656

99.012

0.373