Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQ398_RS15535 Genome accession   NZ_CP053882
Coordinates   3348379..3350085 (+) Length   568 a.a.
NCBI ID   WP_041210692.1    Uniprot ID   A0AAQ0J2H4
Organism   Aeromonas jandaei strain 3036     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3343379..3355085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ398_RS15510 (HQ398_15485) pdhR 3344565..3345329 (-) 765 WP_104015851.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HQ398_RS15515 (HQ398_15490) ampD 3345608..3346177 (-) 570 WP_215805984.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HQ398_RS15520 (HQ398_15495) - 3346300..3346770 (+) 471 WP_201912989.1 retropepsin-like aspartic protease -
  HQ398_RS15525 (HQ398_15500) nadC 3346774..3347631 (+) 858 WP_215805986.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HQ398_RS15530 (HQ398_15505) tapA 3347955..3348371 (+) 417 WP_156853719.1 type IVa pilus major pilin TapA -
  HQ398_RS15535 (HQ398_15510) pilB 3348379..3350085 (+) 1707 WP_041210692.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HQ398_RS15540 (HQ398_15515) pilC 3350231..3351472 (+) 1242 WP_215805988.1 type II secretion system F family protein Machinery gene
  HQ398_RS15545 (HQ398_15520) - 3351495..3352366 (+) 872 Protein_3023 A24 family peptidase -
  HQ398_RS15550 (HQ398_15525) coaE 3352384..3352992 (+) 609 WP_215805990.1 dephospho-CoA kinase -
  HQ398_RS15555 (HQ398_15530) zapD 3353037..3353759 (+) 723 WP_215805992.1 cell division protein ZapD -
  HQ398_RS15560 (HQ398_15535) yacG 3353763..3353963 (+) 201 WP_215805995.1 DNA gyrase inhibitor YacG -
  HQ398_RS15565 (HQ398_15540) mutT 3354036..3354440 (-) 405 WP_041210679.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62537.94 Da        Isoelectric Point: 5.3215

>NTDB_id=448389 HQ398_RS15535 WP_041210692.1 3348379..3350085(+) (pilB) [Aeromonas jandaei strain 3036]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILESKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESESDALGMEDIDESEI
SDLEVSDESSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRMSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=448389 HQ398_RS15535 WP_041210692.1 3348379..3350085(+) (pilB) [Aeromonas jandaei strain 3036]
ATGACCTCCAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCACTGATAAGTGAGGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGTAAACCCTTTGTAACCTTCCTGATCGAGAACGAGATCCTTGAGAGCAAGGCTCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCATCATGTGCTGCCCATATACACCCAAGGGCACACTCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAAGATTTCGGCTTCAGCTTTGGCCTGCATACCGAAGCGCTGCTGGTCGAAGAGAACA
AGCTCACAGCGGCAATAGGAAAATTGCTGGAGAGCGAAAGCGATGCCCTCGGAATGGAGGATATCGATGAATCTGAAATA
TCCGACCTCGAAGTATCCGATGAGAGCAGTCGACTGGATGAAAGCGTCAACACCGCTGATGATGATGCCCCAATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGCGCCTCCGACCTGCACTTTGAGCCCTACGAGCAGAAATATC
GCATTCGCTTTCGTATCGACGGCATCCTGCATGAAGTGGCAACCCCGCCAGTCAATTTGGCCAACCGTTTTTCTGCCCGT
CTCAAGGTCATGGCACGGCTCGATATCGCCGAACGGCGGCTGCCACAGGATGGCCGAATCAAGCTCAAACTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACTCTGCCCACCATGTGGGGCGAGAAAATTGTTATCCGTCTGCTCGACTCATCAG
CTGCCCGCCTCAATATCGAGCAGCTCGGCTTTGATGACCGGCAAAAAGCGCAATATCTGCGTGCACTTTCGAAACCTCAA
GGCATGATCTTGGTCACCGGCCCGACCGGTTCGGGTAAAACGGTCTCGCTCTATACCGGTCTTAATATTCTCAACACTAC
CGAGGTGAACATCTCCACCGCTGAAGATCCGGTGGAGATCAACTTGCCCGGTGTCAACCAGGTACAGGTCAACCCCAAGG
CTGGTCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAGATCCGCGAT
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCAGCCGC
CGAAACCCTCACCCGGATGATGAACATGGGGGTGCCAGCCTTTAATATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GACTGGCACGCAAACTGTGTGACCACTGCAAGGCTCCCGAAGTAGTGCCGGAAGCCGAATTACTGGAGCTGGGCTTTACT
CAGCAGCAACTGGCGGCGGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGCTCTGGTGGATACAAAGGGCGGGT
CGGTATCTACGAGATCATGCTGATGTCGGAAAACATAGCAAAGCTGATCATGCAGGGGGCCAACTCGCTACAAATAGCGG
CTATAGCCCAGAAAGAGGGGATGCGTACCTTGCGTATGTCCGGATTGGAAAAGGCTCGCCTTGGCGTCACCAGTCTGGCA
GAGGTCAACCGGGTGACAACGAATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

100

0.558
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

50.871

100

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.596

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361