Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPY10_RS02365 Genome accession   NZ_CP053822
Coordinates   490437..492125 (-) Length   562 a.a.
NCBI ID   WP_175247443.1    Uniprot ID   -
Organism   Vibrio cholerae strain E7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485437..497125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY10_RS02340 (HPY10_02340) yacG 486503..486700 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY10_RS02345 (HPY10_02345) zapD 486858..487598 (-) 741 WP_000207194.1 cell division protein ZapD -
  HPY10_RS02350 (HPY10_02350) coaE 487630..488238 (-) 609 WP_162810836.1 dephospho-CoA kinase -
  HPY10_RS02355 (HPY10_02355) pilD 488235..489110 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  HPY10_RS02360 (HPY10_02360) pilC 489168..490394 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY10_RS02365 (HPY10_02365) pilB 490437..492125 (-) 1689 WP_175247443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY10_RS02370 (HPY10_02370) pilA 492129..492554 (-) 426 WP_142609993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY10_RS02375 (HPY10_02375) nadC 492799..493689 (-) 891 WP_175245918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY10_RS02380 (HPY10_02380) ampD 493985..494530 (+) 546 WP_175245919.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY10_RS02385 (HPY10_02385) fldB 494701..495219 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY10_RS02390 (HPY10_02390) xerD 495357..496265 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY10_RS02395 (HPY10_02395) dsbC 496282..497034 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62079.16 Da        Isoelectric Point: 5.9427

>NTDB_id=447881 HPY10_RS02365 WP_175247443.1 490437..492125(-) (pilB) [Vibrio cholerae strain E7G]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLSLGIFPAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGVANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNNELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=447881 HPY10_RS02365 WP_175247443.1 490437..492125(-) (pilB) [Vibrio cholerae strain E7G]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGAACTTCGGTGCCCGAGGCGCTGCTCAGTTTGGGCATTTTCCCCGCCCAAGAACTGACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGATCTTAGCCGTTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTTGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGTAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCTGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGCGTTTCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTGAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCACTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGTCACTTAGTGCTTTCCACTCTGCACACCAACTCGGCTGCCGAAACCGT
GATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAATCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTACGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATAACGAGTTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

51.521

93.594

0.482
  pilB Acinetobacter baylyi ADP1

49.812

94.662

0.472
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37

100

0.395
  pilF Thermus thermophilus HB27

40.111

96.263

0.386