Detailed information NTG0446920

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HQN78_RS19690
• Genome accession: NZ_CP053750
• Coordinates: 4239888..4241024 (+)
• Length: 378 a.a.
• NCBI ID: WP_048412756.1
• Uniprot ID: A0A0J6NRP0
• Organism: Chromobacterium sp. Beijing
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4234888..4246024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HQN78_RS19660 (HQN78_19455)	dksA	4235305..4235715 (-)	411	WP_019102526.1	RNA polymerase-binding protein DksA	-
HQN78_RS19665 (HQN78_19460)	-	4236000..4236305 (-)	306	WP_107801226.1	c-type cytochrome	-
HQN78_RS19670 (HQN78_19465)	-	4236398..4236979 (-)	582	WP_107801225.1	YggT family protein	-
HQN78_RS19675 (HQN78_19470)	proC	4236988..4237791 (-)	804	WP_107801224.1	pyrroline-5-carboxylate reductase	-
HQN78_RS19680 (HQN78_19475)	-	4237845..4238537 (-)	693	WP_227105727.1	YggS family pyridoxal phosphate-dependent enzyme	-
HQN78_RS19685 (HQN78_19480)	pilT	4238805..4239842 (+)	1038	WP_043589321.1	type IV pilus twitching motility protein PilT	Machinery gene
HQN78_RS19690 (HQN78_19485)	pilU	4239888..4241024 (+)	1137	WP_048412756.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HQN78_RS19695 (HQN78_19490)	-	4241166..4241753 (-)	588	WP_107801222.1	TetR/AcrR family transcriptional regulator	-
HQN78_RS19700 (HQN78_19495)	-	4242089..4244005 (+)	1917	WP_235085729.1	diguanylate cyclase	-
HQN78_RS19705 (HQN78_19500)	-	4244058..4244765 (+)	708	WP_107802064.1	pseudouridine synthase	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 42296.42 Da
• Isoelectric Point: 6.2382

>NTDB_id=446920 HQN78_RS19690 WP_048412756.1 4239888..4241024(+) (pilU) [Chromobacterium sp. Beijing]
MEKDQASKFILDLLKHATGKNASDVFIAADFPPAMKIDGKITPVAPQPLSAQHTKELVRAVMNDRQTEEFETKKEANFAI
NPPGLGRFRVSAYVQQGHAGMVLRKINTEIPTFDQLNLPLVLRDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSHNHD
HIITIEDPIEYVHQHKKSIITQREVGVDTDDWDIALKNTLRQAPDVILMGEIRDRETMGYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRSIISQRLVPIKSGRGRVAAVEILLNSPLVSDMIFKGEIAGLKEVMGRSRETG
MQTFDQSLFELYEADLISYEDALRNADSINDLRLKIKLYSEGSKSRDPLEGIDHLDIV

Nucleotide

• Length: 1137 bp

>NTDB_id=446920 HQN78_RS19690 WP_048412756.1 4239888..4241024(+) (pilU) [Chromobacterium sp. Beijing]
ATGGAAAAAGACCAGGCTTCCAAGTTCATACTGGATCTGCTGAAGCACGCCACCGGCAAGAACGCCTCCGATGTCTTCAT
CGCCGCCGATTTCCCGCCGGCGATGAAGATAGACGGCAAGATCACACCGGTGGCGCCGCAGCCCCTGTCCGCCCAGCACA
CCAAGGAATTGGTGCGCGCGGTGATGAACGACCGGCAGACCGAGGAGTTCGAAACCAAGAAGGAGGCCAACTTCGCGATC
AATCCGCCGGGCCTGGGGCGCTTCCGCGTCAGCGCCTATGTGCAGCAGGGCCACGCCGGCATGGTGTTGCGCAAGATCAA
CACCGAAATCCCCACTTTCGACCAGCTCAACCTGCCGCTGGTCTTGCGCGACATCGCGCTGATCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCTCGGGCAAATCCACCTCGCTGGCCGCGCTGGTGGACTGGCGCAACAGCCACAACCACGAC
CACATCATCACCATCGAAGATCCGATCGAATACGTGCACCAGCACAAAAAGTCCATCATCACCCAGCGCGAGGTGGGCGT
GGACACCGATGATTGGGACATCGCCTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGGCTACGGCTTGCAGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCC
AACCAGGCGCTGGACCGCATCCTCAACTTCTTCCCCGAGGAGCGTCATCAACAGGTCTTGATGGATTTGTCGCTGAATAT
GCGTTCCATCATTTCCCAGCGGCTGGTGCCGATCAAAAGCGGCCGCGGCCGGGTGGCGGCGGTGGAAATCCTGCTCAACA
GCCCGCTGGTGTCCGACATGATCTTCAAGGGCGAAATCGCCGGTCTGAAGGAAGTGATGGGCCGCTCGCGCGAGACCGGC
ATGCAGACCTTCGACCAGTCCTTGTTCGAGCTGTACGAGGCGGATCTGATCAGCTACGAGGACGCTCTGCGCAACGCCGA
CTCCATCAACGATCTGCGGCTGAAGATCAAGCTTTACAGCGAAGGCTCCAAGTCCAGGGACCCGCTGGAAGGCATAGACC
ACCTCGACATCGTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (17-277)

3D structure

• AlphaFold DB: A0A0J6NRP0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.032	92.328	0.563
pilU	Acinetobacter baylyi ADP1	55.738	96.825	0.54
pilU	Vibrio cholerae strain A1552	53.602	91.799	0.492
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.104	89.153	0.402
pilT	Acinetobacter baylyi ADP1	41.003	89.683	0.368
pilT	Pseudomonas aeruginosa PAK	40.653	89.153	0.362