Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HJ571_RS26565 Genome accession   NZ_CP053747
Coordinates   5657714..5659414 (+) Length   566 a.a.
NCBI ID   WP_179839642.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Pae1255-NDM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5652714..5664414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HJ571_RS26540 (HJ571_26550) nadC 5654519..5655367 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HJ571_RS26550 (HJ571_26560) - 5655548..5656414 (-) 867 WP_225007570.1 O-antigen ligase family protein -
  HJ571_RS26555 - 5656424..5656930 (-) 507 WP_225007572.1 hypothetical protein -
  HJ571_RS26560 (HJ571_26565) - 5657019..5657483 (-) 465 WP_023129290.1 pilin -
  HJ571_RS26565 (HJ571_26570) pilB 5657714..5659414 (+) 1701 WP_179839642.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HJ571_RS26570 (HJ571_26575) pilC 5659418..5660635 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  HJ571_RS26575 (HJ571_26580) pilD 5660636..5661508 (+) 873 WP_034037193.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HJ571_RS26580 (HJ571_26585) coaE 5661505..5662116 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  HJ571_RS26585 (HJ571_26590) yacG 5662113..5662313 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HJ571_RS26590 (HJ571_26595) - 5662350..5662559 (-) 210 WP_003094660.1 hypothetical protein -
  HJ571_RS26595 (HJ571_26600) - 5662665..5663354 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  HJ571_RS26600 (HJ571_26605) - 5663351..5663821 (-) 471 WP_003103866.1 hypothetical protein -
  HJ571_RS26605 (HJ571_26610) - 5663818..5664243 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62329.24 Da        Isoelectric Point: 5.4470

>NTDB_id=446859 HJ571_RS26565 WP_179839642.1 5657714..5659414(+) (pilB) [Pseudomonas aeruginosa strain Pae1255-NDM1]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQTVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=446859 HJ571_RS26565 WP_179839642.1 5657714..5659414(+) (pilB) [Pseudomonas aeruginosa strain Pae1255-NDM1]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGTGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGACGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

51.342

98.763

0.507
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

94.17

0.489
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.601

100

0.41
  pilF Thermus thermophilus HB27

38.489

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.173

0.375