Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPT10_RS24640 Genome accession   NZ_CP053705
Coordinates   5315310..5317010 (+) Length   566 a.a.
NCBI ID   WP_073647569.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PAC6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5310310..5322010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPT10_RS24615 (HPT10_24640) ampD 5310346..5310912 (-) 567 WP_003094640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPT10_RS24620 (HPT10_24645) - 5311063..5313318 (-) 2256 WP_003112843.1 DUF1631 domain-containing protein -
  HPT10_RS24625 (HPT10_24650) nadC 5313604..5314452 (+) 849 WP_023126570.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPT10_RS24635 (HPT10_24660) - 5314606..5315079 (-) 474 WP_015503553.1 pilin -
  HPT10_RS24640 (HPT10_24665) pilB 5315310..5317010 (+) 1701 WP_073647569.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPT10_RS24645 (HPT10_24670) pilC 5317014..5318231 (+) 1218 WP_023126571.1 type II secretion system F family protein Machinery gene
  HPT10_RS24650 (HPT10_24675) pilD 5318234..5319106 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HPT10_RS24655 (HPT10_24680) coaE 5319103..5319714 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HPT10_RS24660 (HPT10_24685) yacG 5319711..5319911 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HPT10_RS24665 (HPT10_24690) - 5319948..5320157 (-) 210 WP_003094660.1 hypothetical protein -
  HPT10_RS24670 (HPT10_24695) - 5320263..5320952 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HPT10_RS24675 (HPT10_24700) - 5320949..5321419 (-) 471 WP_023096122.1 hypothetical protein -
  HPT10_RS24680 (HPT10_24705) - 5321416..5321841 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62357.24 Da        Isoelectric Point: 5.7041

>NTDB_id=446477 HPT10_RS24640 WP_073647569.1 5315310..5317010(+) (pilB) [Pseudomonas aeruginosa strain PAC6]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAVNDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFLE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=446477 HPT10_RS24640 WP_073647569.1 5315310..5317010(+) (pilB) [Pseudomonas aeruginosa strain PAC6]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCTAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAAGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCGTCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGCAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCGCCTGTAGTACGTTT
CGTAAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGAG
TGCGCTTCCGTACCGACGGCATGCTTCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCTGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.58
  pilB Acinetobacter baylyi ADP1

56.661

99.47

0.564
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.622

99.47

0.504
  pilF Neisseria gonorrhoeae MS11

50.356

99.293

0.5
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366