Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HNE05_RS18070 Genome accession   NZ_CP053697
Coordinates   3928348..3930405 (-) Length   685 a.a.
NCBI ID   WP_173209919.1    Uniprot ID   A0A6M8FL71
Organism   Aquipseudomonas campi strain S1-A32-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3923348..3935405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNE05_RS18055 (HNE05_15690) - 3925023..3926660 (-) 1638 WP_173209913.1 AAA family ATPase -
  HNE05_RS18060 (HNE05_15695) aroB 3926671..3927774 (-) 1104 WP_173209915.1 3-dehydroquinate synthase -
  HNE05_RS18065 (HNE05_15700) aroK 3927825..3928343 (-) 519 WP_173209917.1 shikimate kinase AroK -
  HNE05_RS18070 (HNE05_15705) pilQ 3928348..3930405 (-) 2058 WP_173209919.1 type IV pilus secretin PilQ Machinery gene
  HNE05_RS18075 (HNE05_15710) pilP 3930459..3930986 (-) 528 WP_173209921.1 type 4a pilus biogenesis lipoprotein PilP -
  HNE05_RS18080 (HNE05_15715) pilO 3930983..3931606 (-) 624 WP_173209923.1 type 4a pilus biogenesis protein PilO -
  HNE05_RS18085 (HNE05_15720) - 3931606..3932229 (-) 624 WP_173209925.1 PilN domain-containing protein -
  HNE05_RS18090 (HNE05_15725) pilM 3932229..3933293 (-) 1065 WP_173209927.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 685 a.a.        Molecular weight: 74074.20 Da        Isoelectric Point: 4.6126

>NTDB_id=446397 HNE05_RS18070 WP_173209919.1 3928348..3930405(-) (pilQ) [Aquipseudomonas campi strain S1-A32-2]
MNSSLSRLGISLFVAMFAPALLAANLEALDVASLPGDKVELKLTFDEPVAAPRGYTIEQPARIALDMPGVANKLGTKNRE
LGVGNARSVTVVEAKDRTRLIINLSTLVPYSTRADGKNLYVVVGDTANVSAAQSASSAVPQSVQAYAPVGRAIKGIDFQR
GEMGEGNVVIDLSDPTVSPDIQEQGGKIRLDFAKTQLPEALRARLDVKDFATPVQFVNAVAKGDGATVTVEPTGFYDYLV
FQADNKLTISIKPLTQDDVEKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQA
LDLVLKTRGLDKRKVGNVLLVAPADEIAARERQELEAKQQLAELAPLRREVVQLNYAKASDIAKLFEDAGEQGSLDKDMR
GKIIFDDRTNSLIAYLTQERIDELRRIVTQLDIPVRQVMIEARIVEANVDFNKELGVEWRGVGLGDNNWVAGGTNSVEKG
TDPLEFQAGNFVDLGAAGRTAGLNLGFITDNTLLDLELSAMEKTGNGEVISQPKVVTSDKETAKILKGAKIPYPEASSSG
AATTAFAEANLSLEVTPQITPDNRVIIEVKVNKDAADFSNQVNGTPSILTNEVNAKVLVSDGETIVIGGIFSNTQSTAVD
KVPLLGDMPYIGRLFRRDVVQDSKSELLVFLTPRIMNNQAIAVSP

Nucleotide


Download         Length: 2058 bp        

>NTDB_id=446397 HNE05_RS18070 WP_173209919.1 3928348..3930405(-) (pilQ) [Aquipseudomonas campi strain S1-A32-2]
ATGAATAGCTCTTTATCTCGTCTTGGCATTTCGCTTTTTGTTGCCATGTTTGCGCCTGCATTGCTGGCTGCAAACCTAGA
AGCTCTAGATGTAGCTTCGCTACCTGGTGACAAGGTCGAGCTGAAACTCACATTCGACGAGCCTGTTGCCGCCCCGCGTG
GCTACACCATTGAACAACCTGCTCGAATTGCACTGGATATGCCAGGTGTGGCCAATAAGCTGGGTACGAAGAATCGTGAA
CTCGGTGTGGGCAATGCGCGTAGCGTGACAGTGGTCGAGGCAAAAGATCGTACTCGCCTGATTATCAATCTTTCTACTCT
CGTCCCATACAGCACCCGTGCCGATGGCAAGAATCTCTACGTGGTGGTTGGTGATACTGCGAATGTAAGTGCCGCGCAAA
GTGCTTCAAGCGCGGTTCCCCAGTCTGTGCAGGCATATGCCCCCGTCGGTCGTGCAATCAAGGGCATTGACTTCCAGCGC
GGCGAAATGGGAGAGGGGAATGTTGTTATCGATTTGTCCGATCCGACTGTGAGCCCTGACATTCAGGAGCAGGGTGGAAA
AATTCGTTTGGACTTCGCCAAAACCCAGCTGCCGGAAGCGCTGCGTGCCCGCTTGGATGTGAAGGATTTCGCCACCCCTG
TTCAGTTCGTTAATGCAGTGGCCAAGGGCGATGGCGCCACCGTAACTGTGGAGCCTACAGGCTTCTATGATTACCTAGTT
TTCCAGGCGGATAACAAGCTGACCATCAGTATCAAGCCGCTAACTCAGGATGATGTCGAGAAGCGTAAGGCTGAACGTTT
TGCCTACTCCGGCGAGAAGTTGTCGCTGAATTTTCAGGATATTGATGTTCGTTCGGTTCTGCAGCTGATTGCCGATTTCA
CCGATCTCAATCTGGTGGCTAGCGACACGGTGCAGGGCAACATCACTCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCG
CTGGATCTGGTACTGAAGACTCGTGGCCTCGACAAACGCAAGGTGGGCAATGTGCTGCTGGTGGCTCCTGCCGATGAGAT
TGCGGCACGGGAGCGTCAGGAGCTTGAAGCCAAGCAGCAGCTAGCTGAACTTGCTCCGCTGCGTCGGGAAGTTGTTCAGT
TGAACTATGCCAAGGCTTCCGATATCGCCAAGCTGTTCGAAGATGCGGGCGAGCAAGGCTCTCTGGATAAAGACATGCGT
GGCAAGATCATTTTCGATGATCGAACCAACAGCCTCATTGCTTATTTGACTCAGGAGCGCATAGACGAACTGCGTCGGAT
CGTGACTCAGCTCGATATTCCGGTGCGTCAGGTGATGATCGAGGCTCGCATCGTTGAGGCCAATGTGGATTTTAACAAAG
AGCTGGGTGTCGAGTGGCGTGGTGTGGGCCTTGGAGATAACAATTGGGTAGCAGGTGGTACCAATAGTGTCGAGAAAGGC
ACAGATCCGCTTGAGTTTCAGGCCGGTAACTTTGTCGATCTTGGTGCAGCGGGTCGTACTGCTGGGCTGAACCTGGGCTT
CATCACCGATAACACCTTGCTGGATCTTGAGCTGTCGGCCATGGAAAAGACCGGTAACGGTGAAGTCATTTCCCAGCCGA
AAGTCGTTACCTCCGATAAAGAGACCGCCAAGATCCTCAAGGGTGCGAAAATTCCGTACCCGGAAGCCTCCTCCAGCGGT
GCTGCGACTACGGCTTTCGCTGAAGCCAACTTGTCCTTGGAAGTCACCCCGCAGATTACCCCGGATAACCGGGTGATCAT
CGAAGTGAAGGTCAACAAGGATGCGGCAGACTTCTCCAATCAGGTGAATGGCACGCCATCCATCCTGACCAACGAAGTGA
ATGCCAAGGTACTGGTAAGCGATGGTGAGACCATCGTGATCGGCGGTATTTTCAGCAATACGCAGTCCACGGCTGTCGAC
AAGGTGCCGCTGCTGGGCGATATGCCTTACATCGGCCGCCTGTTCCGTCGCGATGTGGTTCAGGACTCCAAGTCTGAACT
GCTGGTGTTCCTCACTCCGCGTATCATGAACAATCAAGCCATTGCAGTGAGCCCGTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(297-343)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(520-676)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8FL71

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.448

100

0.749
  comQ Acinetobacter baylyi ADP1

41.46

100

0.415
  pilQ Acinetobacter baumannii strain A118

39.971

100

0.404
  pilQ Acinetobacter baumannii D1279779

39.971

100

0.404
  pilQ Legionella pneumophila strain Lp02

37.818

97.664

0.369
  pilQ Legionella pneumophila strain ERS1305867

37.668

97.664

0.368