Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HNE05_RS18090 Genome accession   NZ_CP053697
Coordinates   3932229..3933293 (-) Length   354 a.a.
NCBI ID   WP_173209927.1    Uniprot ID   A0A6M8F7L2
Organism   Aquipseudomonas campi strain S1-A32-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3927229..3938293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNE05_RS18065 (HNE05_15700) aroK 3927825..3928343 (-) 519 WP_173209917.1 shikimate kinase AroK -
  HNE05_RS18070 (HNE05_15705) pilQ 3928348..3930405 (-) 2058 WP_173209919.1 type IV pilus secretin PilQ Machinery gene
  HNE05_RS18075 (HNE05_15710) pilP 3930459..3930986 (-) 528 WP_173209921.1 type 4a pilus biogenesis lipoprotein PilP -
  HNE05_RS18080 (HNE05_15715) pilO 3930983..3931606 (-) 624 WP_173209923.1 type 4a pilus biogenesis protein PilO -
  HNE05_RS18085 (HNE05_15720) - 3931606..3932229 (-) 624 WP_173209925.1 PilN domain-containing protein -
  HNE05_RS18090 (HNE05_15725) pilM 3932229..3933293 (-) 1065 WP_173209927.1 pilus assembly protein PilM Machinery gene
  HNE05_RS18095 (HNE05_15730) - 3933478..3935889 (+) 2412 WP_173209929.1 penicillin-binding protein 1A -
  HNE05_RS18100 (HNE05_15735) - 3935974..3937242 (-) 1269 WP_173209931.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37863.15 Da        Isoelectric Point: 4.4982

>NTDB_id=446398 HNE05_RS18090 WP_173209927.1 3932229..3933293(-) (pilM) [Aquipseudomonas campi strain S1-A32-2]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKTGVRSVAV
AVAGSAVITKTIEMESGLNDDELENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVEVLLAACRKENVEVREAALAL
SGLTAKVVDVEAYALERAYGLLAAQLGSGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQMFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FADMALGGKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=446398 HNE05_RS18090 WP_173209927.1 3932229..3933293(-) (pilM) [Aquipseudomonas campi strain S1-A32-2]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTTCTGGGGATCGACATCAGTTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCAGGAAGCCGCTACAAAGTAGAGGCCTACGCTGTCGAGCCGCTCCCCCCAAATGCTGTCGTCGAAAAGAACA
TTGCCGAACTGGAAGGTGTCGGGCAGGCGCTTTCACGGGTGCTGGCCAAGGCCAAGACCGGTGTGCGTTCGGTTGCCGTT
GCCGTTGCCGGCTCTGCGGTGATCACCAAGACGATCGAGATGGAGTCCGGTCTCAACGATGACGAGCTGGAAAATCAGCT
GAAGATCGAAGCAGACCAATACATCCCCTATCCGCTGGAAGAGGTTGCCATCGACTTCGAGGTTCAGGGTGCTTCTGCGC
GTAACCCCGAACGCGTCGAAGTGCTGCTGGCGGCTTGCCGGAAAGAGAACGTCGAAGTTCGTGAGGCAGCCCTGGCTTTG
TCGGGGCTTACTGCCAAGGTCGTCGATGTCGAGGCCTATGCGCTTGAACGTGCCTACGGTTTGCTTGCCGCTCAGTTGGG
CAGTGGTCATGACGAGCTGACGGTTGCAGTAGTCGATATCGGCGCGACCATGACAACGCTGAGTGTTCTGCACAACGGAC
GCACCATCTACACCCGTGAGCAGATGTTTGGTGGTCGTCAGTTGACTGAAGAAATTCAGCGGCGCTACGGGCTGTCGGTA
GAAGAGGCTGGCCTGGCCAAGAAGCAGGGTGGCCTGCCGGATGACTACGACAGTGAAGTGCTGCAGCCATTCAAGGAGGC
TGTAGTGCAGCAGGTTTCTCGTTCGCTGCAGTTCTTTTTCGCTGCCGGGCAGTTCAATGATGTCGACTACATCCTGCTTG
CAGGCGGGACGGCATCTATCCCAGATCTTGATCGACTGATTCAGCAAAAGATCGGTACTCCTACCCAGGTTGCCAACCCA
TTTGCCGATATGGCGCTGGGGGGCAAGGTCAATGCGGGTGCGCTGGCAAGTGACGCACCGTCCTTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8F7L2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472