Detailed information NTG0446204

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HPC60_RS09805
• Genome accession: NZ_CP053671
• Coordinates: 1967137..1967784 (-)
• Length: 215 a.a.
• NCBI ID: WP_179107683.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain G121
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1962137..1972784

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPC60_RS09780 (HPC60_04525)	atpF	1962206..1962712 (-)	507	WP_004255258.1	F0F1 ATP synthase subunit B	-
HPC60_RS09785 (HPC60_04530)	atpB	1962727..1963440 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
HPC60_RS09790 (HPC60_04535)	-	1963485..1963700 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
HPC60_RS09795 (HPC60_04540)	-	1963885..1964661 (-)	777	WP_023188533.1	alpha/beta hydrolase family protein	-
HPC60_RS09800 (HPC60_04545)	comEC	1964946..1967156 (-)	2211	WP_179107682.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HPC60_RS09805 (HPC60_04550)	comEA	1967137..1967784 (-)	648	WP_179107683.1	ComEA family DNA-binding protein	Machinery gene
HPC60_RS09810 (HPC60_04555)	-	1967844..1969205 (-)	1362	WP_025016984.1	ABC transporter permease	-
HPC60_RS09815 (HPC60_04560)	-	1969202..1970134 (-)	933	WP_025016985.1	ABC transporter ATP-binding protein	-
HPC60_RS09820 (HPC60_04565)	-	1970239..1970637 (-)	399	WP_023188528.1	hypothetical protein	-
HPC60_RS09825 (HPC60_04570)	-	1970741..1971304 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
HPC60_RS09830 (HPC60_04575)	-	1971478..1972656 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23658.78 Da
• Isoelectric Point: 4.8659

>NTDB_id=446204 HPC60_RS09805 WP_179107683.1 1967137..1967784(-) (comEA) [Lactococcus lactis subsp. lactis strain G121]
MDKILEKVKEYWKMIVLVVCGLFAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDIIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=446204 HPC60_RS09805 WP_179107683.1 1967137..1967784(-) (comEA) [Lactococcus lactis subsp. lactis strain G121]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCTTTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATATAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381