Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XAV_RS05145 Genome accession   NZ_CP053649
Coordinates   1192983..1194716 (+) Length   577 a.a.
NCBI ID   WP_042824856.1    Uniprot ID   A0A098Q0B6
Organism   Xanthomonas axonopodis pv. vasculorum strain NCPPB 796     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1187983..1199716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XAV_RS05120 (XAV_05140) coaE 1188790..1189425 (-) 636 WP_042823464.1 dephospho-CoA kinase -
  XAV_RS05125 (XAV_05145) - 1189439..1190302 (-) 864 WP_042823466.1 A24 family peptidase -
  XAV_RS05130 (XAV_05150) pilC 1190309..1191565 (-) 1257 WP_042823467.1 type II secretion system F family protein Machinery gene
  XAV_RS05135 (XAV_05155) - 1191919..1192356 (+) 438 WP_042823468.1 pilin -
  XAV_RS05140 (XAV_05160) - 1192520..1192918 (+) 399 WP_280528854.1 pilin -
  XAV_RS05145 (XAV_05165) pilB 1192983..1194716 (+) 1734 WP_042824856.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XAV_RS05150 (XAV_05170) pilR 1194956..1196404 (-) 1449 WP_042823471.1 sigma-54 dependent transcriptional regulator Regulator
  XAV_RS05155 (XAV_05175) - 1196677..1198290 (-) 1614 WP_042823472.1 PAS domain-containing sensor histidine kinase -
  XAV_RS05160 (XAV_05180) sucC 1198521..1199690 (+) 1170 WP_042823473.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62675.80 Da        Isoelectric Point: 5.4589

>NTDB_id=445969 XAV_RS05145 WP_042824856.1 1192983..1194716(+) (pilB) [Xanthomonas axonopodis pv. vasculorum strain NCPPB 796]
MNSVATTNLVGITGIARRLVQDGAVEEAVARSAMDQAAAAKMPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDEEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
AANGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=445969 XAV_RS05145 WP_042824856.1 1192983..1194716(+) (pilB) [Xanthomonas axonopodis pv. vasculorum strain NCPPB 796]
ATGAATAGTGTCGCGACCACAAATTTGGTTGGGATTACAGGAATCGCGCGTCGTTTGGTACAGGATGGTGCGGTCGAAGA
GGCTGTCGCGCGATCTGCAATGGATCAGGCAGCGGCGGCAAAGATGCCTTTGCCGCAATGGTTTGCCGAGAAAAAGTTGG
TCACTGCTTCCCAGCTAGCCGCTGCCAATGCGGTCGAGTTCGGCATGCCACTGCTAGATGTGTCGGCGTTCGACGCCAGC
CAAAACGCGGTCAAGCTGGTTAGTGAGGAGTTGCTCCAGAAGTACCAAGTGTTGCCGCTGTTCAAGCGCGGCAATAGGCT
GTTCGTGGGGGTTAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTCCACACGAACTTGGTAGTCGAGCCCA
TCCTTGTGGATGAAGACCAGATCCGTCGCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCGCTGGGCTCCGCGCTCGGT
GACGACGAAGAGGGCATGGGGGATCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGTGGGGATTCCGGGGTCGA
TGCCAAGGGTGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTACTGGTGGATGCGATCAGGCGAGGAGCCTCCGACA
TCCATTTCGAACCTTATGAAGACGACTACCGGGTGCGCTTGCGGATCGATGGGCTGCTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAATCAGCGCATTGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATTAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCTTGGATGGTAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTATGGGATGGTGCTGGTCACCGGGCCGACCGGCTCGGGCAAGACGGTGTCCCT
GTACACGGCGCTGGGTATCCTCAATGACGAAACGCGCAATATCTCCACGGCTGAGGATCCGGTCGAAATCCGGTTGCCGG
GCGTCAATCAGGTGCAGCAAAACAACAAGCGCGGCATGACCTTTGCTGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTATCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCACGACTAATGAACATGGGTATCGCGCCGTACAACATCA
CCTCCTCGGTGACGTTGGTGATCGCGCAGCGGCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCTTGCCG
GAGCACGCGCTGTTGGCGGAAGGGTTCACGCCAGAGCAAGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
GTGCACCGAGGGCTACAAGGGGCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCGGCGCAGAAGATCGGTATCCGCGATTTGCGTCAATCGGCGTTGATGAAG
GCTGCGAATGGGGTCACCAGCCTGGCAGAGATCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098Q0B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.652

99.653

0.555
  pilB Acinetobacter baumannii D1279779

56.162

98.44

0.553
  pilB Legionella pneumophila strain ERS1305867

51.585

98.44

0.508
  pilB Vibrio cholerae strain A1552

49.22

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.944

98.44

0.482
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.004

97.574

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

35.4

100

0.376