Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FRAAU_RS05820 Genome accession   NC_017033
Coordinates   1269811..1271532 (-) Length   573 a.a.
NCBI ID   WP_014402639.1    Uniprot ID   H8L4F9
Organism   Frateuria aurantia DSM 6220     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1264811..1276532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRAAU_RS05800 (Fraau_1170) - 1265993..1266940 (+) 948 WP_014402635.1 Nudix family hydrolase -
  FRAAU_RS05805 (Fraau_1171) coaE 1266943..1267575 (-) 633 WP_014402636.1 dephospho-CoA kinase -
  FRAAU_RS05810 (Fraau_1172) pilD 1267572..1268423 (-) 852 WP_014402637.1 A24 family peptidase Machinery gene
  FRAAU_RS05815 (Fraau_1173) pilC 1268505..1269752 (-) 1248 WP_014402638.1 type II secretion system F family protein Machinery gene
  FRAAU_RS05820 (Fraau_1174) pilB 1269811..1271532 (-) 1722 WP_014402639.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FRAAU_RS05825 (Fraau_1175) - 1271803..1273614 (-) 1812 WP_014402640.1 DEAD/DEAH box helicase -
  FRAAU_RS05830 (Fraau_1176) - 1273780..1274529 (+) 750 WP_014402641.1 SDR family NAD(P)-dependent oxidoreductase -
  FRAAU_RS05835 (Fraau_1177) rimJ 1274639..1275202 (+) 564 WP_014402642.1 ribosomal protein S5-alanine N-acetyltransferase -
  FRAAU_RS05840 (Fraau_1178) - 1275188..1276516 (-) 1329 WP_014402643.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63196.70 Da        Isoelectric Point: 6.3115

>NTDB_id=44317 FRAAU_RS05820 WP_014402639.1 1269811..1271532(-) (pilB) [Frateuria aurantia DSM 6220]
MSTQFNHASSPLRGLSRRLVDEGLMSEDAIRHAMAEANTQRQTLVSWLQDRQAVDSLSLSEASSREFGIPLVDLAALDMP
ELPAELLNEALISKHQALPIMRRNKRLYVAIADPMQSHVIDEFKFQSSHIIEPLLVEHRQLQQFIHAAVNKLGAALPHID
DSVLSELEHEYGDQEESISGIDANAADDAPIVKFVNKILLDAIRRGASDIHFEPFEQHYRIRLRLDGLLIAVASPPPKLA
PRLSSRLKVMAGLDIAERRVPQDGRIKLNLSRTQAMDFRVSTLPTLFGEKIVLRILDGSSAKLGIDKLGYEEFQKRLYLD
AIHKPYGMVLVTGPTGSGKTVSLYTALNILNTDERNISTVEDPVEIRVEGINQVEQNAKRGMTFAAALRSFLRQDPDVIM
VGEIRDVETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNIISSVTLVIAQRLARRLHSCKRPVELPRHALL
AEGFSETDIDAGLTIYEPVGCEQCNGGYKGRVGIYQVMPLLENIQQIILRGGGAMQIAEAAAEAGIPDLRESALSKVKAG
IISLAEMARITKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=44317 FRAAU_RS05820 WP_014402639.1 1269811..1271532(-) (pilB) [Frateuria aurantia DSM 6220]
ATGTCGACGCAGTTCAATCACGCTTCCAGTCCCCTGAGAGGCCTCTCCCGCCGACTGGTGGACGAAGGCCTGATGTCCGA
AGATGCCATTCGCCATGCCATGGCGGAGGCCAATACGCAAAGACAGACCCTGGTGAGCTGGCTGCAGGACCGGCAGGCCG
TCGACAGCCTCTCGCTGAGCGAGGCCTCGTCCCGCGAGTTCGGTATTCCGCTGGTGGATCTGGCCGCCCTGGACATGCCG
GAGCTGCCGGCCGAGCTGCTCAATGAAGCCCTGATCAGCAAGCATCAGGCACTGCCCATCATGCGGCGCAACAAGCGACT
CTATGTCGCCATCGCCGATCCCATGCAGAGCCATGTGATCGACGAATTCAAGTTCCAGTCCAGTCACATCATCGAACCGC
TGCTGGTCGAGCACCGCCAGCTGCAGCAATTCATCCATGCCGCCGTCAACAAGCTCGGCGCTGCACTGCCGCACATCGAC
GATTCGGTGCTGAGCGAACTGGAGCACGAATACGGTGACCAGGAAGAATCCATCAGCGGCATCGATGCCAATGCCGCCGA
TGACGCGCCCATCGTCAAGTTCGTCAACAAGATCCTGCTTGACGCGATTCGTCGCGGCGCCTCCGACATCCATTTCGAAC
CGTTCGAGCAGCATTACCGGATCCGCCTGCGCCTGGATGGCCTGCTGATCGCCGTGGCCAGCCCGCCTCCCAAGCTGGCT
CCCCGCCTGTCATCGCGGCTCAAGGTCATGGCCGGTCTGGATATCGCCGAGCGGCGAGTGCCGCAGGATGGCCGGATCAA
GCTCAACCTGTCCAGGACCCAGGCGATGGACTTTCGTGTCAGTACCTTGCCGACACTGTTCGGCGAGAAGATCGTACTGC
GGATCCTGGACGGCTCTTCCGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGAGTTCCAGAAGCGACTCTATCTGGAC
GCGATCCACAAGCCCTACGGCATGGTGCTGGTTACCGGCCCGACCGGCTCGGGCAAGACCGTCTCGCTGTATACCGCCCT
GAATATCCTCAATACCGACGAGCGCAATATCTCGACGGTCGAAGATCCGGTGGAAATCCGGGTCGAAGGCATCAACCAGG
TCGAACAGAATGCCAAGCGCGGCATGACGTTCGCCGCCGCCCTGCGCTCGTTCCTGCGACAGGATCCGGATGTGATCATG
GTCGGCGAGATCCGTGACGTGGAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGCCATATGGTGCTGTCCACCCT
GCACACCAATGACGCGCCACAGACCATTGCCCGCCTGATGAACATGGGCATCGCACCTTACAACATCATCTCCTCGGTGA
CCTTGGTGATCGCCCAGCGCCTGGCCCGGCGCCTGCACAGCTGCAAGCGTCCGGTGGAACTGCCGCGCCACGCCCTGCTT
GCCGAAGGCTTCAGCGAGACGGACATCGATGCCGGCCTGACGATCTATGAGCCGGTCGGCTGCGAGCAGTGCAATGGCGG
CTACAAGGGCCGGGTCGGCATCTATCAGGTGATGCCGCTGCTGGAGAATATCCAGCAGATCATCCTGCGTGGCGGCGGCG
CCATGCAGATCGCCGAAGCCGCTGCCGAGGCCGGCATCCCGGATTTGCGCGAATCCGCCCTGAGCAAGGTCAAGGCCGGT
ATCATCAGCCTTGCCGAAATGGCACGCATCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-146)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H8L4F9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.322

99.825

0.532
  pilB Acinetobacter baylyi ADP1

52.753

98.255

0.518
  pilB Legionella pneumophila strain ERS1305867

49.385

99.302

0.49
  pilB Vibrio cholerae strain A1552

51.625

91.274

0.471
  pilF Neisseria gonorrhoeae MS11

47.434

98.604

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.473

92.321

0.466
  pilB Vibrio campbellii strain DS40M4

49.806

90.052

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.522

100

0.366
  pilF Thermus thermophilus HB27

37.23

97.033

0.361

Multiple sequence alignment