Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DSM104443_RS01860 Genome accession   NZ_CP053069
Coordinates   352263..353969 (+) Length   568 a.a.
NCBI ID   WP_171089008.1    Uniprot ID   A0A6M4GUN4
Organism   Usitatibacter rugosus strain 0125_3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347263..358969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104443_RS01835 (DSM104443_00340) - 348056..348286 (+) 231 WP_171089003.1 formate dehydrogenase subunit delta -
  DSM104443_RS01840 (DSM104443_00341) prmC 348283..349104 (-) 822 WP_171089004.1 peptide chain release factor N(5)-glutamine methyltransferase -
  DSM104443_RS01845 (DSM104443_00342) prfA 349104..350186 (-) 1083 WP_171089005.1 peptide chain release factor 1 -
  DSM104443_RS01850 (DSM104443_00343) hemA 350183..351445 (-) 1263 WP_171089006.1 glutamyl-tRNA reductase -
  DSM104443_RS01855 (DSM104443_00344) - 351660..352199 (+) 540 WP_171089007.1 glutathione peroxidase -
  DSM104443_RS01860 (DSM104443_00345) pilB 352263..353969 (+) 1707 WP_171089008.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DSM104443_RS01865 (DSM104443_00346) pilC 353988..355214 (+) 1227 WP_171089009.1 type II secretion system F family protein Machinery gene
  DSM104443_RS01870 (DSM104443_00347) - 355289..356248 (+) 960 WP_171089010.1 A24 family peptidase -
  DSM104443_RS01875 (DSM104443_00348) coaE 356245..356850 (+) 606 WP_212756896.1 dephospho-CoA kinase -
  DSM104443_RS01880 (DSM104443_00349) zapD 356939..357694 (+) 756 WP_171089011.1 cell division protein ZapD -
  DSM104443_RS01885 (DSM104443_00350) - 357691..357885 (+) 195 WP_246232452.1 DNA gyrase inhibitor YacG -
  DSM104443_RS01890 (DSM104443_00351) - 357982..358422 (+) 441 WP_171089012.1 DUF2244 domain-containing protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61934.40 Da        Isoelectric Point: 5.2310

>NTDB_id=442661 DSM104443_RS01860 WP_171089008.1 352263..353969(+) (pilB) [Usitatibacter rugosus strain 0125_3]
MAAAPSHFNALGRALVQQGKLVQADANAIQAEATKAGISFIQQLIGSKKLSAKEVSMFAANTFGYPLLDLNAVDGDQLPT
SLIDAKIVGNHRVVALGKRGNRLFVAMSDPSNEQVMQEVKFSTGMTMEPIVVEDDKLNIFVKKITEGGGKALEKMVEDVD
LQLPDEGELEAETAASVEDIDDAPVVKYVQKVLLDAINAGASDIHFEPYEKFYRIRYRQDGVLYDVAQPPLAIKEKIASR
IKVVSKLDISEKRVPQDGRMKLTLSKTRAIDFRVSTLPTLFGEKICMRILDPSSATLGIDALGYDPDQKENLMTAIQRPY
GMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNINDKAGMTFPAALKAFLRQDPDIIMVGEIRD
IETGEISIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGIAPFNIASSVIMVTAQRLARKLCVCKRPIDIPEEALKRAGFTE
EDLDGTWQPFAPAGCDVCKNTGYKGRVGIYQVMPISEEMERIIMKNGTAIDLADQSRREGIKDLRQSGLIKVKKGLTSLE
EVEGVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=442661 DSM104443_RS01860 WP_171089008.1 352263..353969(+) (pilB) [Usitatibacter rugosus strain 0125_3]
ATGGCCGCAGCGCCTTCCCATTTCAATGCCCTCGGGCGCGCCCTCGTGCAACAGGGCAAGCTCGTACAGGCCGACGCCAA
CGCCATCCAGGCGGAAGCGACGAAGGCGGGTATTTCCTTCATCCAGCAGCTGATCGGATCGAAGAAGCTCTCGGCGAAGG
AAGTCTCGATGTTCGCCGCCAACACCTTCGGCTATCCGCTGCTCGACCTGAACGCGGTCGACGGCGACCAGCTCCCCACC
AGCCTCATCGACGCGAAGATCGTCGGCAACCACCGCGTTGTGGCCCTCGGCAAGCGCGGCAACCGCCTGTTCGTCGCGAT
GAGCGACCCGTCGAACGAGCAGGTGATGCAGGAGGTGAAGTTCTCGACCGGCATGACGATGGAGCCCATCGTCGTCGAGG
ACGACAAGCTCAACATCTTCGTGAAGAAGATCACCGAGGGCGGCGGCAAGGCCCTCGAGAAGATGGTCGAGGACGTCGAC
CTGCAGCTGCCCGACGAGGGCGAGCTCGAGGCCGAGACCGCCGCTTCCGTCGAGGACATCGACGACGCCCCGGTGGTGAA
GTACGTGCAGAAGGTGCTGCTCGACGCCATCAACGCCGGCGCCTCCGACATCCACTTCGAGCCCTACGAGAAGTTCTACC
GCATCCGCTACCGCCAGGACGGCGTGCTCTACGACGTGGCGCAGCCGCCGCTCGCGATCAAGGAGAAGATCGCCTCGCGC
ATCAAGGTCGTCTCCAAGCTCGACATCTCCGAGAAGCGCGTCCCGCAGGACGGGCGCATGAAGCTCACGCTCTCCAAGAC
GCGCGCCATCGACTTCCGCGTCTCGACGCTGCCGACGCTCTTCGGCGAGAAGATTTGCATGCGGATCCTCGATCCCTCGA
GCGCCACGCTCGGGATCGACGCCCTGGGCTACGACCCGGACCAGAAAGAGAACCTGATGACCGCCATCCAGCGCCCCTAC
GGGATGGTGCTGGTGACGGGGCCGACGGGCTCGGGCAAGACGGTGTCGCTCTACACGTGCCTGAACATCCTGAACAAGCC
GGGCATCAATATCTCGACCGCGGAGGACCCGGCGGAGATCAACCTGCCCGGCGTGAACCAGGTCAACATCAACGACAAGG
CGGGGATGACGTTCCCGGCGGCGCTGAAGGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGCGAGATCCGCGAC
ATCGAGACCGGCGAGATCTCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTCTCCACGCTGCACACCAACGACGCGCC
GCAGACGCTCACGCGATTGATGAACATGGGCATCGCTCCGTTCAACATCGCGTCGTCCGTGATCATGGTCACGGCCCAGC
GCCTCGCGCGGAAGCTCTGCGTGTGCAAGCGCCCGATCGACATCCCGGAGGAAGCGCTCAAGCGCGCGGGTTTCACCGAA
GAAGACCTCGACGGAACCTGGCAGCCCTTCGCGCCCGCCGGATGCGACGTATGCAAGAATACCGGCTACAAGGGGCGCGT
CGGCATCTACCAGGTCATGCCCATCTCCGAGGAGATGGAACGCATCATCATGAAGAACGGCACGGCGATCGACCTTGCCG
ACCAGTCCCGCCGCGAAGGCATCAAGGACCTGCGGCAATCGGGGCTCATCAAGGTGAAGAAGGGACTCACTTCCCTCGAG
GAAGTCGAAGGCGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M4GUN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.064

99.648

0.539
  pilB Acinetobacter baylyi ADP1

52.91

99.824

0.528
  pilB Legionella pneumophila strain ERS1305867

51.394

100

0.519
  pilF Neisseria gonorrhoeae MS11

51.601

98.944

0.511
  pilB Vibrio campbellii strain DS40M4

45.035

99.296

0.447
  pilB Vibrio cholerae strain A1552

45.179

98.592

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

44.366

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

35.761

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.114

92.606

0.371
  pilF Thermus thermophilus HB27

36.907

100

0.37