Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HKW99_RS02585 Genome accession   NZ_CP053061
Coordinates   555753..557864 (+) Length   703 a.a.
NCBI ID   WP_407294741.1    Uniprot ID   -
Organism   Stutzerimonas zhaodongensis strain JZ60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550753..562864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW99_RS02565 (HKW99_02550) pilM 552921..553985 (+) 1065 WP_407294734.1 pilus assembly protein PilM Machinery gene
  HKW99_RS02570 (HKW99_02555) pilN 553985..554557 (+) 573 WP_407294736.1 type 4a pilus biogenesis protein PilN -
  HKW99_RS02575 (HKW99_02560) pilO 554554..555177 (+) 624 WP_407294738.1 type 4a pilus biogenesis protein PilO -
  HKW99_RS02580 (HKW99_02565) pilP 555174..555701 (+) 528 WP_407294740.1 type 4a pilus biogenesis lipoprotein PilP -
  HKW99_RS02585 (HKW99_02570) pilQ 555753..557864 (+) 2112 WP_407294741.1 type IV pilus secretin PilQ Machinery gene
  HKW99_RS02590 (HKW99_02575) aroK 557869..558414 (+) 546 WP_407294743.1 shikimate kinase AroK -
  HKW99_RS02595 (HKW99_02580) aroB 558438..559529 (+) 1092 WP_407294744.1 3-dehydroquinate synthase -
  HKW99_RS02600 (HKW99_02585) - 559550..561070 (+) 1521 WP_407294746.1 AAA family ATPase -

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75578.63 Da        Isoelectric Point: 4.8055

>NTDB_id=442587 HKW99_RS02585 WP_407294741.1 555753..557864(+) (pilQ) [Stutzerimonas zhaodongensis strain JZ60]
MNTTFTRLGLSLLAVFISPALLAANLNSLDVASLPGERVELKLAFDEPVAAPRGYTLDQPARIALDLPGVSNKLGSKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGNDLFVVLGESAVSQAAVAAPVTTAVPAATPVSQVAVGKSISNID
FRRGEDGAGNVVITLSDPSISPNIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDSSSKADGASIAIEPSGRYD
YLAYQTDDKLTISVKPLSDAEAEKQKAEHFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESKRQIAELAPLRREVVQVNYAKAADIAKLFQSVTAVDGQAD
ERGSISVDDRTNNIIAYQTQERLDELRRIVTQLDIPVRQVMIEARIVEANVGYDKALGVRWGGNVGNGRWSAYGKDGARS
IDDDGVLAFPGSKTVGNFTLEDGVAPVPFVDLGVLNSTSGVGIGFISDHLLLDLQLSAMEKTGNGEVVSQPKVMTADKET
AKILKGTEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFRNAVNGVPPIDKNEVNAKVLVNDG
ETVVIGGVFSNSQSKSVDKVPFLGDVPYVGRLFRRDVVTDAKSELLVFLTPRILNHQAIAVNR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=442587 HKW99_RS02585 WP_407294741.1 555753..557864(+) (pilQ) [Stutzerimonas zhaodongensis strain JZ60]
ATGAATACCACTTTCACTCGTCTCGGTTTGTCCTTGCTGGCGGTGTTTATTTCGCCTGCACTGCTAGCTGCGAATCTGAA
CTCATTGGACGTCGCGTCCCTGCCTGGCGAACGCGTTGAGCTGAAGCTGGCGTTTGACGAGCCGGTGGCTGCCCCGCGTG
GCTATACGCTTGACCAGCCCGCCCGCATCGCGCTCGATCTGCCAGGCGTATCGAATAAGTTAGGGTCAAAGAACCGCGAG
CTTGGAGTCGGTAACGCACGCAGTGTCACGGTCGTAGAGGCCCAGGGGCGAACCCGGCTGATCGTGAACCTCACTTCGCT
GGTTCCTTATTCCACCCGTGTGGACGGTAACGATTTATTTGTCGTGCTTGGCGAAAGTGCAGTATCGCAAGCTGCTGTTG
CTGCTCCGGTGACCACAGCGGTGCCAGCGGCAACTCCTGTCTCCCAGGTAGCCGTTGGCAAGTCAATCAGTAATATTGAT
TTCCGCCGTGGTGAGGATGGTGCGGGTAACGTGGTTATTACACTGTCGGATCCCTCGATCAGCCCGAACATCGAAGAGCA
AGGCGGCAAGATCCGCGTTTCATTTGCCAAGACGCAGCTGCCAGAATCGCTGCGTGTGCGCCTTGATGTTCAGGACTTCG
CAACGCCGGTCAAATTTGTTGATTCGAGCAGCAAGGCTGATGGAGCAAGTATCGCGATCGAGCCTAGCGGGCGATACGAC
TACTTGGCCTACCAGACTGACGACAAGCTGACGATCAGTGTCAAGCCGCTGAGTGATGCGGAGGCCGAGAAGCAAAAGGC
CGAGCATTTCACGTATTCCGGCGAGAAGCTTTCGCTTAACTTCCAAGACATTGACGTACGCTCCGTGCTGCAGCTGATCG
CGGATTTCACCGATCTCAATCTGGTGGCCAGTGATACTGTTCAGGGCAACATCACTCTGCGCTTGCAGAACGTACCTTGG
GACCAGGCGCTGGATCTGGTATTGAAAACGAAGGGCCTCGACAAGCGACAGGTAGGTAACGTCTTGCTGGTAGCACCGGC
GGATGAGATCGCTGCTCGTGAGCGGCAGGAGCTTGAATCCAAGCGGCAAATCGCCGAATTGGCACCGCTTCGCCGTGAAG
TTGTCCAGGTCAATTACGCAAAAGCAGCCGATATTGCGAAGCTCTTCCAGTCGGTTACTGCAGTAGATGGGCAGGCTGAC
GAGCGCGGCTCTATCTCGGTCGATGATCGCACCAACAATATTATTGCTTACCAGACTCAGGAGCGTCTGGATGAGCTGCG
CCGCATTGTGACTCAGCTAGATATTCCTGTCCGCCAGGTCATGATCGAAGCTCGGATTGTTGAGGCAAACGTTGGGTACG
ATAAGGCCCTCGGGGTTCGTTGGGGCGGTAACGTTGGGAATGGTCGATGGAGTGCTTACGGCAAGGACGGCGCCAGATCA
ATAGATGACGACGGAGTCTTAGCGTTTCCGGGAAGCAAGACAGTAGGTAATTTCACACTTGAGGACGGTGTGGCCCCAGT
TCCTTTCGTGGACTTGGGAGTGCTAAACAGCACGTCGGGTGTGGGCATCGGATTTATTTCAGATCACCTCCTGTTGGACC
TGCAGCTTTCCGCCATGGAGAAGACTGGAAACGGGGAAGTGGTATCCCAGCCGAAGGTCATGACAGCTGACAAGGAAACA
GCCAAAATCCTGAAGGGTACGGAAGTGCCATATCAGGAGGCCAGTTCAAGTGGTGCGACAAGTACGTCATTCAAGGAGGC
GGCACTGTCTTTGGAGGTTACTCCTCAAATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTGACCAAAGATGCAC
CGGATTTCCGTAACGCAGTGAACGGCGTTCCTCCAATCGACAAGAACGAAGTCAATGCCAAAGTATTGGTCAACGATGGC
GAAACTGTAGTCATTGGTGGTGTATTCTCTAACAGTCAGTCGAAGAGCGTCGACAAGGTCCCGTTTCTGGGTGATGTACC
CTATGTTGGCCGCTTGTTTCGTCGCGATGTCGTGACAGATGCCAAGTCTGAGCTTCTGGTATTTTTGACCCCTAGGATCC
TGAATCACCAGGCTATCGCAGTGAACCGCTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(538-694)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.23

100

0.754
  comQ Acinetobacter baylyi ADP1

42.286

99.573

0.421
  pilQ Acinetobacter baumannii strain A118

40.027

100

0.42
  pilQ Acinetobacter baumannii D1279779

40.027

100

0.42
  pilQ Legionella pneumophila strain Lp02

36.571

99.573

0.364
  pilQ Legionella pneumophila strain ERS1305867

37.026

97.582

0.361