Detailed information NTG0442586

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HKW99_RS02565
• Genome accession: NZ_CP053061
• Coordinates: 552921..553985 (+)
• Length: 354 a.a.
• NCBI ID: WP_407294734.1
• Uniprot ID: -
• Organism: Stutzerimonas zhaodongensis strain JZ60
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 547921..558985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKW99_RS02550 (HKW99_02535)	rpmE	548032..548244 (+)	213	WP_407294730.1	50S ribosomal protein L31	-
HKW99_RS02555 (HKW99_02540)	-	548924..550192 (+)	1269	WP_407294731.1	malic enzyme-like NAD(P)-binding protein	-
HKW99_RS02560 (HKW99_02545)	-	550302..552737 (-)	2436	WP_407294732.1	penicillin-binding protein 1A	-
HKW99_RS02565 (HKW99_02550)	pilM	552921..553985 (+)	1065	WP_407294734.1	pilus assembly protein PilM	Machinery gene
HKW99_RS02570 (HKW99_02555)	pilN	553985..554557 (+)	573	WP_407294736.1	type 4a pilus biogenesis protein PilN	-
HKW99_RS02575 (HKW99_02560)	pilO	554554..555177 (+)	624	WP_407294738.1	type 4a pilus biogenesis protein PilO	-
HKW99_RS02580 (HKW99_02565)	pilP	555174..555701 (+)	528	WP_407294740.1	type 4a pilus biogenesis lipoprotein PilP	-
HKW99_RS02585 (HKW99_02570)	pilQ	555753..557864 (+)	2112	WP_407294741.1	type IV pilus secretin PilQ	Machinery gene
HKW99_RS02590 (HKW99_02575)	aroK	557869..558414 (+)	546	WP_407294743.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37814.19 Da
• Isoelectric Point: 4.6939

>NTDB_id=442586 HKW99_RS02565 WP_407294734.1 552921..553985(+) (pilM) [Stutzerimonas zhaodongensis strain JZ60]
MLRLFTKKSNTLLGIDISSTSVKLLELSRSGSRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKAKTGAKAAAV
AVSGSAVITKSIEMEAGLGDDDLENQLKIEADQYIPYPLEEVAIDFEVQGPASRSPGRVEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYGLLAPQLGAGHDELTVALVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFHDVDYILLAGGTASIPGLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPSLMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=442586 HKW99_RS02565 WP_407294734.1 552921..553985(+) (pilM) [Stutzerimonas zhaodongensis strain JZ60]
GTGCTAAGGCTCTTCACTAAGAAATCGAATACGCTGCTTGGGATCGACATCAGTTCGACATCCGTCAAGCTGCTGGAATT
AAGCCGCTCAGGTTCCCGATATCGGGTTGAGTCGTATGCGGTCGAGCCACTTCCGGCCAATGCCGTTGTGGAAAAGAACA
TTGCTGAGTTGGAGGGCGTAGGTCAGGCGCTTTCCCGTTTGCTGGCCAAAGCAAAAACTGGAGCCAAGGCCGCAGCTGTT
GCGGTATCCGGTTCAGCAGTGATCACCAAAAGTATCGAAATGGAAGCTGGGCTCGGTGATGATGACCTCGAGAACCAGCT
GAAAATCGAAGCGGATCAGTACATCCCGTATCCGCTCGAGGAAGTCGCAATCGACTTCGAGGTGCAGGGCCCTGCATCTC
GTTCTCCGGGTCGTGTCGAGGTTCTGCTGGCCGCGTGTCGTAAAGAAAACGTGGAGATACGTGAAGCGGCTCTAGCCTTG
GCAGGCCTGACAGCCAAGGTCGTGGATGTCGAAGCTTATGCCTTGGAGCGCTCGTATGGTTTGCTGGCGCCTCAGCTGGG
GGCTGGACATGACGAGCTGACGGTGGCACTCGTCGATATCGGTGCGACCATGACTACGCTAAGCGTGCTGCACAACGGCC
GTACTATCTACACCCGCGAGCAACTGTTCGGTGGGCGACAGCTGACTGAGGAAATTCAGCGTCGTTACGGTTTGTCGATG
GAAGAGGCTGGCTTGGCTAAGAAGCAGGGCGGTCTTCCAGATGACTACGACAGTGAGGTGTTGCAGCCGTTCAAGGAAGC
GGTGGTTCAGCAGGTCTCGCGTTCGCTGCAGTTCTTCTTTGCTGCTGGCCAGTTCCATGATGTGGATTACATCTTGCTCG
CTGGCGGGACTGCTTCAATCCCAGGGCTGGATCGGCTCATTCAGCAGAAAATCGGAACCCAGACTTTAGTCGCGAACCCG
TTCGCCGATATGGCATTGAGCAGCAAGGTTAACGCTGGCGCGCTTGCCAGTGATGCGCCTTCCCTGATGATTGCTTGTGG
GTTGGCCTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.78	100	0.568
comM	Acinetobacter nosocomialis M2	56.78	100	0.568
comM	Acinetobacter baylyi ADP1	54.802	100	0.548
pilM	Legionella pneumophila strain ERS1305867	47.458	100	0.475