Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HKW96_RS25285 Genome accession   NZ_CP053057
Coordinates   5641551..5642768 (+) Length   405 a.a.
NCBI ID   WP_023969386.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain RP4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5636551..5647768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW96_RS25270 (HKW96_25270) - 5636995..5639922 (+) 2928 WP_036984759.1 DUF748 domain-containing protein -
  HKW96_RS25275 (HKW96_25275) - 5640125..5640481 (-) 357 WP_009050906.1 BON domain-containing protein -
  HKW96_RS25280 (HKW96_25280) - 5640769..5641185 (-) 417 WP_009050907.1 pilin -
  HKW96_RS25285 (HKW96_25285) pilC 5641551..5642768 (+) 1218 WP_023969386.1 type II secretion system F family protein Machinery gene
  HKW96_RS25290 (HKW96_25290) pilD 5642771..5643643 (+) 873 WP_023969387.1 A24 family peptidase Machinery gene
  HKW96_RS25295 (HKW96_25295) coaE 5643640..5644263 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  HKW96_RS25300 (HKW96_25300) yacG 5644260..5644463 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  HKW96_RS25305 (HKW96_25305) - 5644468..5644683 (-) 216 WP_009050911.1 hypothetical protein -
  HKW96_RS25310 (HKW96_25310) - 5644749..5645438 (-) 690 WP_023969388.1 energy-coupling factor ABC transporter permease -
  HKW96_RS25315 (HKW96_25315) - 5645435..5645902 (-) 468 WP_023969389.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44386.11 Da        Isoelectric Point: 10.4090

>NTDB_id=442544 HKW96_RS25285 WP_023969386.1 5641551..5642768(+) (pilC) [Pseudomonas chlororaphis strain RP4]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLVRAQLRKQGISPARVRKKSSSPFQRTPPIKARDVSLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLAELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=442544 HKW96_RS25285 WP_023969386.1 5641551..5642768(+) (pilC) [Pseudomonas chlororaphis strain RP4]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGGTCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCCGTTCCAGCGCACGCCCCCGATCAAGGCCCGCGACGTCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGTGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGTTGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGTTTCACCAGTGCGCTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGCGTGTTCGGCCTGCGT
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGGCCTGCTGAAACTGCCGTTGGTGGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCCCTGGTGGAAGCCCTGG
GCTCGGTCGCCGGAGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTCGCGACTGGCATG
CAGCTGCACTTTTCCATGCGCGCGTCCGGTATCTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTGGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.679

100

0.657
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.902

100

0.553
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilG Neisseria meningitidis 44/76-A

41.294

99.259

0.41
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393