Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ISU84_RS20420 Genome accession   NZ_CP064103
Coordinates   4240988..4242373 (+) Length   461 a.a.
NCBI ID   WP_022645178.1    Uniprot ID   -
Organism   Escherichia coli strain IR53017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4235988..4247373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISU84_RS20395 (ISU84_20395) aroP 4236536..4237909 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  ISU84_RS20400 (ISU84_20400) ampE 4237952..4238806 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  ISU84_RS20405 (ISU84_20405) ampD 4238803..4239354 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ISU84_RS20410 (ISU84_20410) nadC 4239442..4240335 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ISU84_RS20415 (ISU84_20415) pilA 4240538..4240978 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  ISU84_RS20420 (ISU84_20420) pilB 4240988..4242373 (+) 1386 WP_022645178.1 type II secretion system protein GspE Machinery gene
  ISU84_RS20425 (ISU84_20425) hofC 4242363..4243565 (+) 1203 WP_022645177.1 protein transport protein HofC -
  ISU84_RS20430 (ISU84_20430) guaC 4243600..4244643 (-) 1044 WP_001217337.1 GMP reductase -
  ISU84_RS20435 (ISU84_20435) - 4244799..4244843 (-) 45 WP_120795372.1 protein YacM -
  ISU84_RS20440 (ISU84_20440) coaE 4244868..4245488 (+) 621 WP_022645176.1 dephospho-CoA kinase -
  ISU84_RS20445 (ISU84_20445) zapD 4245488..4246231 (+) 744 WP_001194731.1 cell division protein ZapD -
  ISU84_RS20450 (ISU84_20450) yacG 4246241..4246438 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  ISU84_RS20455 (ISU84_20455) mutT 4246537..4246926 (-) 390 WP_022645175.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50712.40 Da        Isoelectric Point: 6.5399

>NTDB_id=442354 ISU84_RS20420 WP_022645178.1 4240988..4242373(+) (pilB) [Escherichia coli strain IR53017]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNKLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQEEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=442354 ISU84_RS20420 WP_022645178.1 4240988..4242373(+) (pilB) [Escherichia coli strain IR53017]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGCCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACAAGCTGGGGATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCGGCACTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGAGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.312

83.514

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Vibrio cholerae strain A1552

46.891

83.731

0.393
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.786

88.286

0.36