Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HIR79_RS12270 Genome accession   NZ_CP053032
Coordinates   2594096..2595895 (+) Length   599 a.a.
NCBI ID   WP_169958582.1    Uniprot ID   A0A6M4G1C6
Organism   Halomonas sp. PGE1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2589096..2600895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR79_RS12235 (HIR79_12245) - 2589119..2589373 (-) 255 WP_169958575.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
  HIR79_RS12240 (HIR79_12250) - 2589491..2590711 (-) 1221 WP_169958576.1 RNA-guided endonuclease TnpB family protein -
  HIR79_RS12245 (HIR79_12255) - 2590896..2591327 (-) 432 WP_169958577.1 low molecular weight protein-tyrosine-phosphatase -
  HIR79_RS12250 (HIR79_12260) - 2591339..2592781 (-) 1443 WP_169958578.1 MBL fold metallo-hydrolase -
  HIR79_RS12255 (HIR79_12265) - 2593110..2593256 (+) 147 WP_169958579.1 hypothetical protein -
  HIR79_RS12260 (HIR79_12270) - 2593367..2593792 (+) 426 WP_169958580.1 nucleotidyltransferase domain-containing protein -
  HIR79_RS12265 (HIR79_12275) - 2593782..2594003 (+) 222 WP_169958581.1 HepT-like ribonuclease domain-containing protein -
  HIR79_RS12270 (HIR79_12280) pilB 2594096..2595895 (+) 1800 WP_169958582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HIR79_RS12275 (HIR79_12285) pilC 2595948..2597183 (+) 1236 WP_169958583.1 type II secretion system F family protein Machinery gene
  HIR79_RS12280 (HIR79_12290) pilD 2597205..2598053 (+) 849 WP_169959573.1 A24 family peptidase Machinery gene
  HIR79_RS12285 (HIR79_12295) - 2598186..2598764 (-) 579 WP_119020348.1 hypoxanthine-guanine phosphoribosyltransferase -
  HIR79_RS12290 (HIR79_12300) nagZ 2598810..2599817 (-) 1008 WP_169958584.1 beta-N-acetylhexosaminidase -
  HIR79_RS12295 (HIR79_12310) - 2599962..2600675 (-) 714 WP_249322141.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 599 a.a.        Molecular weight: 65214.79 Da        Isoelectric Point: 5.5448

>NTDB_id=442323 HIR79_RS12270 WP_169958582.1 2594096..2595895(+) (pilB) [Halomonas sp. PGE1]
MNDYRTPPSAPLPGSSQGSALESTRLDATAGLRGLAWRLVADERISHAAAVRAEEEAREGEVSLLEHVIDTGLVSARQAT
LSAAWEYGLPMMDLDAIRPETLPRADAFPEKILRKLKVLPLVQRGHRLTVAVPFPSTLALLDELQFATGLTPEGVLAPVD
QLGPVLESYLASSEKSMLDELKGVDDAIGELGFAGEEESGEVDVQAAASAASEDAPIIKFVNKILLDAIHRGASDIHFEP
YETSYRVRFRIDGMLHDVAHPPFAMRARIAARLKVMSRLDISERRLPQDGAIKLRLSKTRSIDFRVNSLPTVYGEKVVLR
ILDPASAQLGIEQLGFTPEQRAMYETVLDQPQGMILVTGPTGSGKTVTLYTGINILNEVERNICTAEDPVEIKVPGVNQV
NVIPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGLAPFNIASSVS
LIIAQRLARKLCKHCKQPADLPHEALAAQGFTEEEINQATFFEPVGCKHCTKGFKGRVGIYEVVPISHAMSQLIMKNGNS
MEFSDLARQEGYPDLRRSGLMKAMQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1800 bp        

>NTDB_id=442323 HIR79_RS12270 WP_169958582.1 2594096..2595895(+) (pilB) [Halomonas sp. PGE1]
ATGAACGACTATCGCACCCCGCCGAGCGCCCCGCTCCCGGGCTCTTCACAGGGCTCGGCCCTGGAGAGCACCCGCCTTGA
TGCCACCGCGGGGCTGCGCGGCCTGGCGTGGCGCCTGGTGGCGGACGAGCGCATCAGCCACGCCGCGGCGGTGCGCGCCG
AGGAAGAGGCCCGCGAGGGCGAGGTGTCACTGCTGGAGCACGTGATCGACACCGGGCTCGTCTCCGCCCGCCAGGCCACC
CTCTCGGCGGCCTGGGAGTATGGCCTGCCGATGATGGACCTCGACGCCATCCGCCCCGAGACATTACCCCGCGCCGATGC
CTTCCCCGAGAAGATCCTGCGCAAGCTCAAGGTGCTGCCGTTGGTGCAGCGCGGCCACCGGCTCACCGTGGCGGTGCCCT
TCCCCTCCACGCTTGCCCTGCTCGACGAGCTGCAGTTCGCCACCGGCCTGACCCCAGAGGGGGTGCTGGCCCCGGTGGAC
CAGCTCGGCCCGGTGCTGGAGAGCTATCTCGCCTCCAGTGAGAAGAGCATGCTCGATGAGCTCAAGGGGGTCGACGATGC
CATCGGCGAGCTGGGCTTCGCCGGCGAAGAGGAGAGCGGCGAGGTGGACGTGCAGGCCGCGGCGAGCGCGGCCAGCGAAG
ACGCGCCGATCATCAAGTTCGTCAACAAGATCCTGCTCGACGCCATCCACCGCGGCGCCTCGGACATCCACTTCGAGCCG
TATGAAACCAGCTACCGGGTGCGCTTTCGCATCGACGGCATGCTGCATGACGTGGCCCACCCACCCTTCGCCATGCGCGC
GCGCATCGCCGCACGCCTCAAGGTGATGTCGCGGCTCGATATCTCGGAGCGCCGCCTGCCCCAGGACGGCGCCATCAAGC
TCCGGCTCTCCAAGACCCGCTCCATCGACTTTCGCGTCAACTCGCTGCCCACGGTGTATGGCGAGAAGGTGGTACTGCGG
ATCCTCGACCCCGCCTCGGCCCAGCTCGGCATCGAGCAGCTCGGCTTCACGCCGGAGCAGCGCGCCATGTACGAGACGGT
GCTGGACCAGCCCCAGGGCATGATCCTGGTGACCGGGCCCACCGGCAGCGGCAAGACGGTGACCCTCTACACCGGCATCA
ACATCCTCAACGAGGTGGAGCGCAACATCTGCACCGCCGAGGACCCGGTGGAGATCAAGGTGCCCGGGGTCAACCAGGTG
AACGTGATCCCCAAGATCGGCCTGGACTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGT
GGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGCGGTAAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCCACGGTGC
ACACCAACTCCGCCGCGGAGACCCTCACCCGCCTGGCCAACATGGGCCTGGCGCCGTTCAATATCGCCAGTTCCGTGAGC
CTGATCATCGCCCAGCGCCTGGCGCGCAAGCTCTGCAAGCACTGCAAGCAGCCGGCGGACCTGCCCCATGAGGCCCTGGC
CGCCCAGGGCTTCACGGAGGAGGAGATCAATCAGGCCACCTTCTTCGAGCCGGTGGGCTGCAAGCACTGCACGAAGGGCT
TCAAGGGCCGGGTGGGCATCTACGAGGTGGTGCCGATCAGCCACGCCATGAGTCAGCTGATCATGAAGAATGGCAATTCC
ATGGAGTTCAGCGACCTGGCACGCCAGGAGGGTTACCCGGACTTGCGCAGAAGCGGCCTGATGAAGGCGATGCAGGGGGT
GACCAGCCTGGCCGAGATCAACAGGGTGACCAAAGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(71-174)

Type II/IV secretion system protein (T2SSE)

(223-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M4G1C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.289

94.825

0.496
  pilB Legionella pneumophila strain ERS1305867

52.021

94.992

0.494
  pilB Acinetobacter baumannii D1279779

50.427

97.663

0.492
  pilB Vibrio campbellii strain DS40M4

48.901

91.152

0.446
  pilB Vibrio cholerae strain A1552

49.72

89.316

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

47.905

91.653

0.439
  pilF Neisseria gonorrhoeae MS11

45.263

95.159

0.431
  pilB/pilB1 Synechocystis sp. PCC 6803

39.488

91.319

0.361