Detailed information NTG0442325

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HIR79_RS12280
• Genome accession: NZ_CP053032
• Coordinates: 2597205..2598053 (+)
• Length: 282 a.a.
• NCBI ID: WP_169959573.1
• Uniprot ID: A0A6M4G4L5
• Organism: Halomonas sp. PGE1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2592205..2603053

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HIR79_RS12255 (HIR79_12265)	-	2593110..2593256 (+)	147	WP_169958579.1	hypothetical protein	-
HIR79_RS12260 (HIR79_12270)	-	2593367..2593792 (+)	426	WP_169958580.1	nucleotidyltransferase domain-containing protein	-
HIR79_RS12265 (HIR79_12275)	-	2593782..2594003 (+)	222	WP_169958581.1	HepT-like ribonuclease domain-containing protein	-
HIR79_RS12270 (HIR79_12280)	pilB	2594096..2595895 (+)	1800	WP_169958582.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HIR79_RS12275 (HIR79_12285)	pilC	2595948..2597183 (+)	1236	WP_169958583.1	type II secretion system F family protein	Machinery gene
HIR79_RS12280 (HIR79_12290)	pilD	2597205..2598053 (+)	849	WP_169959573.1	A24 family peptidase	Machinery gene
HIR79_RS12285 (HIR79_12295)	-	2598186..2598764 (-)	579	WP_119020348.1	hypoxanthine-guanine phosphoribosyltransferase	-
HIR79_RS12290 (HIR79_12300)	nagZ	2598810..2599817 (-)	1008	WP_169958584.1	beta-N-acetylhexosaminidase	-
HIR79_RS12295 (HIR79_12310)	-	2599962..2600675 (-)	714	WP_249322141.1	TetR/AcrR family transcriptional regulator	-
HIR79_RS12300 (HIR79_12315)	lexA	2600896..2601546 (+)	651	WP_169958585.1	transcriptional repressor LexA	-
HIR79_RS12305 (HIR79_12320)	dinB	2601567..2602616 (+)	1050	WP_169958586.1	DNA polymerase IV	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 30472.69 Da
• Isoelectric Point: 7.9243

>NTDB_id=442325 HIR79_RS12280 WP_169959573.1 2597205..2598053(+) (pilD) [Halomonas sp. PGE1]
MDLPPSLLWPLAAFVGLCLGSFLNVVIVRLPVMLMLGWRAEAREALELPSETQPRFNLATPRSMCPSCETPIAWHDNLPV
LGWLKRRGRCANCDAAISPQYPLVELAGGALTLAVVALFGPSWQGLFVLGACLALLAMAVIDLRTQLLPDVLTLPLLWAG
LLYQLLFQPLLLSNAVIGAMAGYLTLWSFYWAFKLITGKEGMGYGDFKLLAALGAWMGWQALPLLLILSAGVGAIIGILV
QLAVPRLRGAPMPFGPYLALAGWVALLAGEPLMAAYGRFFML

Nucleotide

• Length: 849 bp

>NTDB_id=442325 HIR79_RS12280 WP_169959573.1 2597205..2598053(+) (pilD) [Halomonas sp. PGE1]
CTGGATCTTCCCCCTTCCCTGCTCTGGCCGCTGGCGGCCTTCGTCGGCCTGTGCCTGGGCAGCTTCCTCAACGTGGTGAT
CGTGCGCCTGCCGGTGATGCTGATGCTGGGCTGGCGGGCCGAGGCCCGCGAGGCGCTGGAGCTGCCGAGTGAGACGCAGC
CGAGATTCAACCTGGCCACACCACGCTCGATGTGCCCGAGCTGCGAGACCCCCATCGCCTGGCACGACAACCTCCCGGTG
CTGGGCTGGCTCAAGCGCCGCGGGCGCTGCGCGAACTGCGATGCGGCCATCAGCCCCCAGTATCCGCTGGTGGAACTGGC
CGGCGGGGCGCTGACGCTCGCCGTGGTGGCGCTGTTCGGGCCGAGCTGGCAGGGGCTCTTCGTGCTGGGAGCCTGCCTTG
CGCTGCTCGCCATGGCGGTGATCGACCTGCGCACCCAGCTCCTGCCGGATGTGCTCACCCTGCCGCTGCTGTGGGCGGGG
CTTCTCTACCAGCTGCTGTTCCAGCCGCTACTGCTTTCGAACGCGGTGATCGGCGCCATGGCGGGGTATCTCACGCTGTG
GAGCTTCTACTGGGCCTTCAAGCTGATCACCGGCAAGGAAGGGATGGGCTACGGCGACTTCAAGCTGCTGGCCGCGCTGG
GTGCCTGGATGGGCTGGCAGGCTCTGCCACTGCTGCTGATCCTCTCCGCCGGGGTGGGGGCCATCATCGGCATCCTGGTG
CAGCTGGCGGTGCCGCGGCTGCGCGGCGCCCCCATGCCCTTCGGGCCCTATCTGGCCCTGGCGGGCTGGGTGGCGCTGCT
GGCCGGGGAGCCGCTGATGGCGGCCTATGGAAGGTTCTTCATGCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (132-238)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (15-117)

3D structure

• AlphaFold DB: A0A6M4G4L5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.137	96.099	0.511
pilD	Vibrio campbellii strain DS40M4	49.643	99.291	0.493
pilD	Acinetobacter baumannii D1279779	48.54	97.163	0.472
pilD	Acinetobacter nosocomialis M2	47.81	97.163	0.465
pilD	Neisseria gonorrhoeae MS11	45.522	95.035	0.433