Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DB356_RS01960 Genome accession   NZ_CP053030
Coordinates   425012..427117 (+) Length   701 a.a.
NCBI ID   WP_010438770.1    Uniprot ID   -
Organism   Pseudomonas congelans strain 508     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420012..432117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB356_RS01940 (DB356_02135) pilM 422181..423245 (+) 1065 WP_003414236.1 type IV pilus assembly protein PilM Machinery gene
  DB356_RS01945 (DB356_02140) - 423245..423820 (+) 576 WP_032613284.1 PilN domain-containing protein -
  DB356_RS01950 (DB356_02145) pilO 423817..424440 (+) 624 WP_010438765.1 type 4a pilus biogenesis protein PilO -
  DB356_RS01955 (DB356_02150) pilP 424437..424964 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  DB356_RS01960 (DB356_02155) pilQ 425012..427117 (+) 2106 WP_010438770.1 type IV pilus secretin PilQ Machinery gene
  DB356_RS01965 (DB356_02160) aroK 427122..427640 (+) 519 WP_024684306.1 shikimate kinase AroK -
  DB356_RS01970 (DB356_02165) aroB 427756..428859 (+) 1104 WP_214423545.1 3-dehydroquinate synthase -
  DB356_RS01975 (DB356_02170) - 428870..430501 (+) 1632 WP_010438779.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75366.82 Da        Isoelectric Point: 5.0992

>NTDB_id=442284 DB356_RS01960 WP_010438770.1 425012..427117(+) (pilQ) [Pseudomonas congelans strain 508]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLATKSRD
LGSGNAHSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGRTNISGTS
NWSAGGLDNRGDEAGNTGNSLGPNIPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=442284 DB356_RS01960 WP_010438770.1 425012..427117(+) (pilQ) [Pseudomonas congelans strain 508]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAACTGGCATTCGACGCCCCGGTGCCTGCGCCGCGGG
GCTACACCACGGCGCAACCGGCGCGCATTGCGCTGGACCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGCCGAGAC
CTGGGCAGTGGCAATGCGCACAGTGTCGTCGTGGTACAAGCTCAGGACCGCACTCGGGTGATCATCAACCTGACCACTCT
TTCGCCTTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGCCGCTGCGCAAGCCTCCC
AGCCGACCACTGCAATGGGCGCGCAACGGGTCGCGGTACCGCCGCCCACGCCAGCATCCGCCAAGCCATTTATCCCTGCA
GGCGTCAAGGCCATCAGGAACGTCGACTTCCAGCGTGGCGAGCTGGGTGAGGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATCCAGGAGCAGGGCGGCAAGATTCGCGTGGACTTCGCCAAGACCCAGTTGCCCGAACCGCTGC
GCGTCCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGATTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTGCGTCCATTGAC
CAACGAGGACCTGGACCGCCGCGCTGCCGACAAGCCGGTGTATACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTCCAGGGCGGTATC
ACGCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGGT
CGGCAACGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAACGTCAGGAGCTGGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCTTGCGCCGCGAGCTGCTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAATCCAAGGCCGACGAGCGCGGCTCGATCACGGTGGACGACCGGACCAATAACATCATTGCCTACCA
GACCCAGGAGCGACTCGACGAACTGCGTCGTATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCAC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTGGGGGTTCGCTGGGGCGGTAGAACCAACATCTCGGGAACCAGT
AACTGGAGCGCGGGAGGCCTGGACAACAGGGGCGACGAGGCCGGCAACACCGGCAACAGCCTGGGGCCGAACATTCCCTT
CGTCGATCTGGGTGCCGCGGATGCGACGACCGGCATCGGTCTCGGCTTCGTGACCAACAACACGCTGCTGGATCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACGACGGTGAGTTTCAAGGAGGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCCGACAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGCGTACCGCCCATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.79

100

0.752
  comQ Acinetobacter baylyi ADP1

42.158

99.144

0.418
  pilQ Acinetobacter baumannii strain A118

38.063

100

0.398
  pilQ Acinetobacter baumannii D1279779

38.063

100

0.398
  pilQ Legionella pneumophila strain ERS1305867

38.978

97.718

0.381
  pilQ Legionella pneumophila strain Lp02

38.686

97.718

0.378