Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DB356_RS01940 Genome accession   NZ_CP053030
Coordinates   422181..423245 (+) Length   354 a.a.
NCBI ID   WP_003414236.1    Uniprot ID   A0A0P9RSF6
Organism   Pseudomonas congelans strain 508     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417181..428245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB356_RS01930 (DB356_02120) - 418044..419312 (+) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -
  DB356_RS01935 (DB356_02130) - 419530..421974 (-) 2445 WP_096131834.1 penicillin-binding protein 1A -
  DB356_RS01940 (DB356_02135) pilM 422181..423245 (+) 1065 WP_003414236.1 type IV pilus assembly protein PilM Machinery gene
  DB356_RS01945 (DB356_02140) - 423245..423820 (+) 576 WP_032613284.1 PilN domain-containing protein -
  DB356_RS01950 (DB356_02145) pilO 423817..424440 (+) 624 WP_010438765.1 type 4a pilus biogenesis protein PilO -
  DB356_RS01955 (DB356_02150) pilP 424437..424964 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  DB356_RS01960 (DB356_02155) pilQ 425012..427117 (+) 2106 WP_010438770.1 type IV pilus secretin PilQ Machinery gene
  DB356_RS01965 (DB356_02160) aroK 427122..427640 (+) 519 WP_024684306.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38120.65 Da        Isoelectric Point: 4.7049

>NTDB_id=442283 DB356_RS01940 WP_003414236.1 422181..423245(+) (pilM) [Pseudomonas congelans strain 508]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=442283 DB356_RS01940 WP_003414236.1 422181..423245(+) (pilM) [Pseudomonas congelans strain 508]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTTGAGCCATTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTCGGTCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
TAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTTGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAGCGCGTCGAGGTGTTGCTGGCGGCCTGCCGCAAAGAGAACGTCGAGGTGCGCGAAGCCGCGCTGGCGCTG
GCCGGACTGACTGCGCGGGTCGTCGATGTCGAGGCCTACGCCCTGGAGCGTTCGTTCGGGCTGCTGGCCGCGCAACTGGG
CAACGGTCATGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGTGTGCTGCATCATGGCC
GGATCATTTACACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCTGACGACTACGTCAGCGAGGTGCTCGATCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCGCTGCAGTTCTTCTTTGCAGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGCCGCATAGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAACGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9RSF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466