Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHE93_RS02075 Genome accession   NZ_CP051529
Coordinates   459244..460956 (-) Length   570 a.a.
NCBI ID   WP_259214674.1    Uniprot ID   -
Organism   Shewanella baltica strain 11FHM2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454244..465956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHE93_RS02045 (HHE93_02070) mutT 454734..455126 (-) 393 WP_259214668.1 8-oxo-dGTP diphosphatase MutT -
  HHE93_RS02050 (HHE93_02075) yacG 455203..455412 (-) 210 WP_006083355.1 DNA gyrase inhibitor YacG -
  HHE93_RS02055 (HHE93_02080) zapD 455486..456220 (-) 735 WP_259214670.1 cell division protein ZapD -
  HHE93_RS02060 (HHE93_02085) coaE 456213..456830 (-) 618 WP_259214671.1 dephospho-CoA kinase -
  HHE93_RS02065 (HHE93_02090) pilD 456870..457784 (-) 915 WP_259214672.1 A24 family peptidase Machinery gene
  HHE93_RS02070 (HHE93_02095) pilC 457824..459095 (-) 1272 WP_259214673.1 type II secretion system F family protein Machinery gene
  HHE93_RS02075 (HHE93_02100) pilB 459244..460956 (-) 1713 WP_259214674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HHE93_RS02080 (HHE93_02105) - 461275..462696 (+) 1422 WP_259214675.1 O-antigen ligase -
  HHE93_RS02085 (HHE93_02110) - 462744..463166 (-) 423 WP_310454465.1 type IV pilin protein -
  HHE93_RS02090 (HHE93_02115) nadC 463847..464728 (-) 882 WP_226058331.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHE93_RS02095 (HHE93_02125) ampD 465264..465812 (+) 549 WP_259214676.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62849.33 Da        Isoelectric Point: 6.2570

>NTDB_id=439507 HHE93_RS02075 WP_259214674.1 459244..460956(-) (pilB) [Shewanella baltica strain 11FHM2]
MPSTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLISARAIAELCYEEYGTPLLDLTEFDLASIPEE
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDRRSEESTGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQTQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVIHGVTSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=439507 HHE93_RS02075 WP_259214674.1 459244..460956(-) (pilB) [Shewanella baltica strain 11FHM2]
ATGCCATCTACAGGCCTTCACTTAGGGCTGTCGACCCTTTTTATCCGCAAAGGTCTGCTAAATGAAGAGCAGATGGCTAA
CGCTATCACTAAATCTCGACAAAACAAACACGCCCTTGTAAGCACCTTAGTTCAAAGTAAGCTAATCTCTGCCCGTGCAA
TAGCCGAGCTTTGCTATGAGGAATATGGCACTCCACTTCTCGATCTCACTGAATTTGATCTTGCTTCCATCCCTGAAGAA
TTTCTTAATAAAAAACTCATTGAAAAACATCGTTGCTTACCCTTGTTCAAGCGTGGGAATCGCCTCTATATAGGCACATC
CGACCCAACCAATATTGCCGCCCTTGAAGACTTCCAATTTAGTGCAGGCCTGCATGCCGAAGCGATTCTGGTTGAAGAAG
ATAAACTTGCTAAAGCGTTGGAGAAAATTCTCGAAGAAGATATTAGTGGTCTCGATTTAGGCGGCATGGACGAAGCAGCG
CTGGCAGCGATAGAAATTACTGATACAGACAGACGTAGCGAAGAGTCTACTGGTGAGAGTAGTGACGATGCGCCTATTGT
CATTTATATCAATAAAATTTTGACAGATGCTATTCGTAAGGGTGCGTCGGATCTACACTTTGAACCCTATGAGAAACGCT
ATCGCATACGGTTTCGTATAGATGGTATTCTGCACGAAGTCTCTGAACCGCCGATTAGTCTGGCTGGTCGTCTTTCTGCA
CGCTTAAAAGTGATGTCAAAACTCGATATCGCCGAACGGCGCGTGCCACAGGATGGTCGAATTAAGATGAAGCTTTCCCG
CACTAAATCTATCGACTTTCGGGTTAGCACTCTGCCGACCCTGTGGGGTGAAAAGATTGTAATGCGGATTTTGGACTCGT
CCTCAGCACAACTAGGCATCGAAAAACTCGGCTACGAGCCCGACCAAGAAAAACTCTACCTTGAGATGCTAGCAAAACCC
CAAGGGATGATTTTAGTGACGGGTCCAACGGGCTCAGGTAAGACCGTCTCCCTTTACACAGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATTTCTACCGCAGAAGATCCGGTTGAAATTAACCTTGAAGGTGTGAACCAAGTTCACATTAATTTAA
AGGCAGGCCTCACCTTCGCATCCGCTTTACGTTCATTTCTACGCCAAGACCCTGATGTGGTGATGGTGGGCGAAATCCGC
GATCTGGAAACAGCCGAAATTGCCATTAAAGCGGCGCAAACAGGACACTTAGTCTTATCGACCTTGCACACAAACTCGGC
TGCCGAAACCTTAACCCGTTTAATCAACATGGGTGTACCTGGCTATAACATAGCTAGCTCTGTGAATTTGATTATTGCCC
AGCGCTTAGCGCGTCGTCTCTGCCCCGAATGTAAACAAGCTGAAGTGATACCCGAGCATGAATTACTGCGCCTTGGTTTT
GCCCAAACACAAATTACTCAAGGCTTTACCACCTTTAAACCCATTGGCTGCGAACATTGCTCTGCGGGCTACAAGGGGCG
GGTTGGCATTTATGAAGTGATGAAAATGTCTGACGAAATAGCCCGCACTATCATGGAAGGGGGGAACTCTTTACAAATCG
CCTCCCAAGCTAAAGCGCAAGGAATGCGGGATTTGCGTCAATCAGGATTATTGAAAGTGATCCACGGTGTTACTAGTATT
GCAGAAGTCAATCGCGTAACCAGTTTTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.18

98.421

0.582
  pilB Acinetobacter baumannii D1279779

58.716

95.614

0.561
  pilB Legionella pneumophila strain ERS1305867

55.694

98.596

0.549
  pilB Vibrio cholerae strain A1552

53.514

97.368

0.521
  pilB Vibrio parahaemolyticus RIMD 2210633

51.573

100

0.518
  pilB Vibrio campbellii strain DS40M4

51.327

99.123

0.509
  pilF Neisseria gonorrhoeae MS11

47.173

99.298

0.468
  pilF Thermus thermophilus HB27

38.681

98.421

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.71

97.895

0.379