Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INP70_RS02325 Genome accession   NZ_CP063420
Coordinates   487175..487585 (-) Length   136 a.a.
NCBI ID   WP_000649335.1    Uniprot ID   -
Organism   Vibrio cholerae strain VC_hf7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482175..492585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP70_RS02305 coaE 482673..483281 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  INP70_RS02310 pilD 483278..484153 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  INP70_RS02315 pilC 484211..485437 (-) 1227 WP_000648396.1 type II secretion system F family protein Machinery gene
  INP70_RS02320 pilB 485480..487168 (-) 1689 WP_000957207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INP70_RS02325 pilA 487175..487585 (-) 411 WP_000649335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  INP70_RS02330 nadC 487830..488720 (-) 891 WP_000665277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  INP70_RS02335 ampD 489018..489563 (+) 546 WP_000567314.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP70_RS02340 fldB 489712..490230 (-) 519 WP_000690112.1 flavodoxin FldB -
  INP70_RS02345 xerD 490368..491276 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  INP70_RS02350 dsbC 491293..492045 (+) 753 WP_000115054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14248.54 Da        Isoelectric Point: 9.0659

>NTDB_id=439422 INP70_RS02325 WP_000649335.1 487175..487585(-) (pilA) [Vibrio cholerae strain VC_hf7]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPAYKDYVKKSQASSALATLKSLVTPAELLIQSDGSISGGLSVLGISAG
SNTLGTLSVGTGNKSLVFVFKNGDLANANLTMTREDTGWVCERSDKTKIPPIEGCQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=439422 INP70_RS02325 WP_000649335.1 487175..487585(-) (pilA) [Vibrio cholerae strain VC_hf7]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGTGTGTT
GGCAGCGATTGCAGTACCTGCTTACAAAGATTACGTCAAAAAAAGCCAAGCTTCATCGGCGTTAGCTACCTTGAAAAGCC
TCGTAACACCGGCAGAACTACTCATTCAATCGGATGGCTCTATTAGTGGTGGTCTGAGCGTTTTAGGTATATCCGCAGGT
TCAAATACTTTAGGAACGCTTTCTGTTGGTACCGGCAACAAATCTTTAGTATTCGTATTCAAGAATGGTGATCTGGCAAA
CGCGAACCTCACAATGACTCGCGAAGATACAGGATGGGTTTGTGAGAGAAGTGATAAAACAAAAATTCCACCTATTGAAG
GTTGCCAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(41-130)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50

92.647

0.463
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.611

100

0.456
  pilA Vibrio cholerae strain A1552

41.611

100

0.456
  pilA Vibrio cholerae C6706

41.611

100

0.456
  pilA Pseudomonas aeruginosa PAK

38.514

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.732

100

0.404
  pilA Acinetobacter baumannii strain A118

37.778

99.265

0.375