Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HGP31_RS02295 Genome accession   NZ_CP051487
Coordinates   512314..514365 (+) Length   683 a.a.
NCBI ID   WP_168757101.1    Uniprot ID   -
Organism   Pseudomonas umsongensis strain CY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507314..519365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGP31_RS02275 (HGP31_02275) pilM 509535..510596 (+) 1062 WP_020796149.1 pilus assembly protein PilM Machinery gene
  HGP31_RS02280 (HGP31_02280) - 510596..511159 (+) 564 WP_168757100.1 PilN domain-containing protein -
  HGP31_RS02285 (HGP31_02285) - 511156..511776 (+) 621 WP_033042556.1 type 4a pilus biogenesis protein PilO -
  HGP31_RS02290 (HGP31_02290) - 511776..512300 (+) 525 WP_033042554.1 pilus assembly protein PilP -
  HGP31_RS02295 (HGP31_02295) pilQ 512314..514365 (+) 2052 WP_168757101.1 type IV pilus secretin PilQ Machinery gene
  HGP31_RS02300 (HGP31_02300) aroK 514370..514888 (+) 519 WP_032831512.1 shikimate kinase AroK -
  HGP31_RS02305 (HGP31_02305) aroB 515040..516140 (+) 1101 WP_020796154.1 3-dehydroquinate synthase -
  HGP31_RS02310 (HGP31_02310) - 516151..517740 (+) 1590 WP_168757102.1 AAA family ATPase -

Sequence

Protein


Download         Length: 683 a.a.        Molecular weight: 74063.31 Da        Isoelectric Point: 5.4236

>NTDB_id=438947 HGP31_RS02295 WP_168757101.1 512314..514365(+) (pilQ) [Pseudomonas umsongensis strain CY-1]
MNRIFSTLGVSLWIALLSPMVQAANLKALDVAALPGDRVELKLVFDQPPPTPRGYTTDSPARIALDLPGVVSQLASKTRD
LGSGNARSATVVEAKDRARLIINLTQLSPYDMRVEGNNLFVVVGQGGKSRAPRAAAAAVPVPVPARAVAPTGKAIRAVDF
QRGTQGEGNVVIDLSDPSIAPDIQEREGKIVLGFNKTRLPEPLRVRLDVKDFATPVQFVNASATGDKATITIEPNGAFDY
STYQTDNKLTVSIRPMTVDDLQKRNADRNAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWD
QALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTNAEAKADE
RGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVGNGTS
DGGSTSSPFVDLGVANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEASSSGAT
SVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQSKVVDKV
PFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2052 bp        

>NTDB_id=438947 HGP31_RS02295 WP_168757101.1 512314..514365(+) (pilQ) [Pseudomonas umsongensis strain CY-1]
ATGAACAGGATTTTCTCCACCCTCGGAGTATCGCTATGGATAGCGTTGCTGTCACCGATGGTACAAGCGGCCAACCTCAA
GGCGCTGGATGTCGCCGCGTTGCCGGGAGATCGCGTCGAGTTGAAGCTGGTGTTCGACCAACCGCCTCCGACGCCCCGTG
GTTATACAACGGACTCTCCTGCCCGGATTGCCCTGGATCTGCCCGGTGTCGTCAGTCAGCTGGCGAGCAAAACTCGCGAC
CTGGGCAGTGGCAATGCCCGCAGCGCGACCGTGGTGGAGGCCAAGGATCGTGCGCGGCTGATCATCAACCTGACCCAGTT
GAGCCCCTACGACATGCGGGTCGAAGGCAATAACCTGTTTGTGGTCGTCGGGCAGGGAGGTAAAAGCCGTGCGCCAAGAG
CGGCCGCTGCTGCTGTACCGGTCCCGGTTCCGGCGCGCGCGGTTGCACCGACAGGCAAAGCGATTCGTGCGGTGGATTTC
CAGCGGGGTACCCAGGGCGAAGGCAATGTCGTGATCGACTTGTCGGATCCGTCCATTGCACCCGATATCCAGGAGCGCGA
AGGCAAGATCGTTCTCGGCTTCAACAAGACCCGATTGCCCGAGCCGTTGCGTGTGCGCCTGGATGTGAAGGATTTCGCCA
CACCGGTGCAATTCGTCAATGCCAGTGCCACGGGGGACAAGGCGACCATCACCATAGAACCCAACGGTGCCTTTGACTAT
TCGACCTACCAGACCGACAACAAGCTGACTGTCAGCATTCGTCCGATGACGGTCGACGACCTGCAAAAGCGCAACGCCGA
TCGCAATGCCTACAGCGGTGAAAAGCTTTCGCTGAATTTCCAAGACATCGACGTGCGCTCGGTACTGCAACTGATCGCCG
ATTTCACCAACCTCAATCTCGTCGCCAGTGACACGGTCCAGGGGGGAATTACCCTGCGCCTGCAAAACGTACCCTGGGAC
CAGGCGCTGGACCTGGTACTGAAAACCAAGGGCCTGGACAAGCGCAAGCTGGGTAATGTGTTGCTCGTCGCTCCGGCCGA
CGAAATTGCCGCCCGTGAACGCCAGGAGCTGGAGTCGCAAAAGCAGATTGCCGACCTGGCACCACTGCGTCGGGAGCTGT
TGCAGGTCAATTACGCCAAGGCGGCCGATATCGCCAAACTGTTCCAGTCGGTGACCAACGCCGAGGCGAAAGCCGACGAA
CGGGGATCGATCACGGTCGATGAACGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAACTGCGGCG
CATCGTTGCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGCGTATCGTTGAAGCCAACGTTGACTACGACA
AGAGCCTTGGCGTGCGTTGGGGAGGCTCAATACAGAACAAAGGCAACTGGAATGCCTCGGGAGTCGGTAACGGCACCAGC
GACGGCGGTAGCACCAGCTCGCCGTTCGTCGACCTGGGGGTCGCCAACAACACATCGGGCATCGGCATCGCCTTCATCAC
CGACAACGTCTTGCTGGACCTTGAATTGACGGCCATGGAAAAGACCGGCAATGGCGAAATCGTCTCGCAACCCAAGGTCG
TCACTTCCGACAAGGAAACCGCAAAGATCCTCAAGGGCACCGAGATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCTACT
TCGGTCTCGTTCAAAGAGGCTTCGCTATCGCTGGAGGTGACGCCGCAGATCACGCCGGACAACCGGATCATCATGGAGGT
CAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAGGTCCAGGATGTGCCGCCGATCAAGAAAAACGAAGTCAATGCCA
AGGTCCTGGTCAACGATGGCGAGACCATCGTCATTGGTGGTGTTTTCTCAAATACTCAAAGCAAGGTTGTAGATAAGGTG
CCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGTTGGT
GTTTCTCACACCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(518-674)

Secretin and TonB N terminus short domain (STN)

(299-345)

AMIN domain (AMIN)

(39-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.761
  comQ Acinetobacter baylyi ADP1

41.57

100

0.419
  pilQ Acinetobacter baumannii D1279779

38.389

100

0.419
  pilQ Acinetobacter baumannii strain A118

38.389

100

0.419
  pilQ Legionella pneumophila strain Lp02

39.021

98.682

0.385
  pilQ Legionella pneumophila strain ERS1305867

38.872

98.682

0.384