Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HGP31_RS02275 Genome accession   NZ_CP051487
Coordinates   509535..510596 (+) Length   353 a.a.
NCBI ID   WP_020796149.1    Uniprot ID   -
Organism   Pseudomonas umsongensis strain CY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504535..515596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGP31_RS02260 (HGP31_02260) rpmE 504864..505082 (+) 219 WP_008047849.1 50S ribosomal protein L31 -
  HGP31_RS02265 (HGP31_02265) - 505369..506637 (+) 1269 WP_020796147.1 malic enzyme-like NAD(P)-binding protein -
  HGP31_RS02270 (HGP31_02270) - 506879..509320 (-) 2442 WP_033042561.1 penicillin-binding protein 1A -
  HGP31_RS02275 (HGP31_02275) pilM 509535..510596 (+) 1062 WP_020796149.1 pilus assembly protein PilM Machinery gene
  HGP31_RS02280 (HGP31_02280) - 510596..511159 (+) 564 WP_168757100.1 PilN domain-containing protein -
  HGP31_RS02285 (HGP31_02285) - 511156..511776 (+) 621 WP_033042556.1 type 4a pilus biogenesis protein PilO -
  HGP31_RS02290 (HGP31_02290) - 511776..512300 (+) 525 WP_033042554.1 pilus assembly protein PilP -
  HGP31_RS02295 (HGP31_02295) pilQ 512314..514365 (+) 2052 WP_168757101.1 type IV pilus secretin PilQ Machinery gene
  HGP31_RS02300 (HGP31_02300) aroK 514370..514888 (+) 519 WP_032831512.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37985.72 Da        Isoelectric Point: 4.7353

>NTDB_id=438946 HGP31_RS02275 WP_020796149.1 509535..510596(+) (pilM) [Pseudomonas umsongensis strain CY-1]
MLRLFNKKARSLLGIDISSTSVKLLELSRQGNGYRVEAYAVEPLPAKAVIEKNIAELDGVGQALARVLVKARTDLKYVAV
AVAGSAVITKTIEMDAGLTDDEMEDQLKIEADQYIPYPLDDVAIDFEVQGASTRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLKIQQGGAQGRLVAVVDVGATMTTLSVLLEGRIIYTREQLFGGRQLTEAIQRRYGLTVE
QAGLAKRQGGLPDDYVGEVLQPFREALVQQVSRSLQFFFASGQYSAVDQILLAGGTASIPGLERLIEQQLGTPTLVANPF
CDMTLGSKVNAVALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=438946 HGP31_RS02275 WP_020796149.1 509535..510596(+) (pilM) [Pseudomonas umsongensis strain CY-1]
GTGCTAAGACTCTTCAATAAAAAAGCCAGGTCGCTCCTGGGAATCGATATCAGCTCCACTTCGGTGAAGTTGCTGGAACT
GAGCCGCCAGGGCAATGGCTATCGGGTCGAAGCCTACGCAGTCGAGCCGTTGCCCGCCAAGGCGGTCATCGAGAAAAATA
TCGCCGAGCTCGATGGGGTGGGCCAGGCGCTGGCGCGCGTGCTGGTCAAGGCCAGGACCGACCTCAAGTACGTAGCGGTG
GCCGTGGCCGGTTCTGCAGTGATCACCAAGACCATTGAAATGGATGCCGGCCTTACCGATGACGAGATGGAAGACCAGTT
GAAGATCGAGGCCGACCAGTACATCCCTTATCCGCTGGACGATGTCGCCATCGATTTCGAAGTCCAGGGCGCTTCCACGC
GCAATCCTGAGCGGGTCAACGTGTTACTTGCCGCCTGTCGCAAGGAAAATGTCGAGGTCCGTGAGGCCGCACTGGCCCTG
GCGGGCTTGACTGCGCGCGTGGTCGACGTGGAGGCCTATGCCCTGGAGCGTTCGTTCGGTTTGCTCAAGATCCAGCAGGG
AGGCGCTCAAGGGCGCTTGGTGGCAGTAGTCGATGTCGGAGCAACCATGACCACCCTGAGCGTGCTGCTTGAGGGGCGGA
TTATCTATACCCGAGAGCAATTGTTCGGCGGCCGTCAGCTCACCGAGGCAATTCAGCGCCGATATGGCCTGACCGTTGAG
CAGGCCGGCTTGGCGAAGAGACAAGGCGGCTTGCCGGATGATTATGTCGGCGAAGTGCTGCAGCCGTTTCGCGAAGCGCT
GGTACAGCAAGTATCGCGCTCATTGCAGTTCTTCTTCGCTTCGGGCCAGTACAGCGCGGTCGACCAGATTCTGCTGGCCG
GCGGTACTGCGTCAATCCCCGGTCTGGAGCGGTTGATCGAGCAGCAATTGGGAACGCCAACGCTGGTGGCCAACCCGTTT
TGCGACATGACGTTGGGCAGCAAGGTCAATGCCGTGGCCCTGGCCAGCGATGCGCCGGCGCTGATGATTGCCTGCGGGTT
GGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.239

100

0.535
  comM Acinetobacter nosocomialis M2

53.239

100

0.535
  comM Acinetobacter baylyi ADP1

52.394

100

0.527
  pilM Legionella pneumophila strain ERS1305867

47.632

100

0.484