Detailed information NTG0438422

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HC661_RS12355
• Genome accession: NZ_CP051463
• Coordinates: 2544135..2544749 (-)
• Length: 204 a.a.
• NCBI ID: WP_015417854.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain UCMB5140
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2539135..2549749

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC661_RS12325 (HC661_23640)	rpsT	2539438..2539704 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HC661_RS12330 (HC661_23650)	holA	2539721..2540762 (-)	1042	Protein_2396	DNA polymerase III subunit delta	-
HC661_RS12335	-	2540802..2540953 (-)	152	Protein_2397	hypothetical protein	-
HC661_RS12340	-	2540994..2541128 (+)	135	WP_003152870.1	YqzM family protein	-
HC661_RS12345 (HC661_23660)	comEC	2541147..2543498 (-)	2352	WP_015417853.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HC661_RS12350 (HC661_23670)	-	2543499..2544068 (-)	570	WP_003152868.1	ComE operon protein 2	-
HC661_RS12355 (HC661_23680)	comEA	2544135..2544749 (-)	615	WP_015417854.1	helix-hairpin-helix domain-containing protein	Machinery gene
HC661_RS12360 (HC661_23690)	comER	2544808..2545629 (+)	822	WP_015417855.1	late competence protein ComER	-
HC661_RS12365 (HC661_23700)	-	2545698..2546435 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
HC661_RS12370 (HC661_23710)	rsfS	2546432..2546788 (-)	357	WP_007408260.1	ribosome silencing factor	-
HC661_RS12375 (HC661_23720)	yqeK	2546806..2547366 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HC661_RS12380 (HC661_23730)	-	2547356..2547925 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
HC661_RS12385 (HC661_23740)	yhbY	2547936..2548226 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HC661_RS12390 (HC661_23750)	aroE	2548220..2549062 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22207.39 Da
• Isoelectric Point: 6.4888

>NTDB_id=438422 HC661_RS12355 WP_015417854.1 2544135..2544749(-) (comEA) [Bacillus velezensis strain UCMB5140]
MTKAMMTERLRRNLKILVACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=438422 HC661_RS12355 WP_015417854.1 2544135..2544749(-) (comEA) [Bacillus velezensis strain UCMB5140]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.653	96.078	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA	Lactococcus lactis subsp. cremoris KW2	34.685	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae R6	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368