Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HF908_RS03295 Genome accession   NZ_CP051171
Coordinates   696442..697707 (-) Length   421 a.a.
NCBI ID   WP_193027555.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2279     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 691442..702707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF908_RS03265 (HF908_03305) - 692513..693397 (+) 885 WP_193027551.1 ATP-binding protein -
  HF908_RS03270 (HF908_03310) - 693375..693836 (+) 462 WP_193027552.1 NUDIX domain-containing protein -
  HF908_RS03275 (HF908_03315) yacG 693829..694044 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  HF908_RS03280 (HF908_03320) zapD 694055..694813 (-) 759 WP_020747465.1 cell division protein ZapD -
  HF908_RS03285 (HF908_03325) coaE 694931..695545 (-) 615 WP_193027553.1 dephospho-CoA kinase -
  HF908_RS03290 (HF908_03330) - 695558..696439 (-) 882 WP_275760905.1 prepilin peptidase -
  HF908_RS03295 (HF908_03335) pilC 696442..697707 (-) 1266 WP_193027555.1 type II secretion system F family protein Machinery gene
  HF908_RS03300 (HF908_03340) pilB 697752..699479 (-) 1728 WP_193027556.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HF908_RS03305 (HF908_03345) - 699727..701004 (-) 1278 WP_193027557.1 HlyC/CorC family transporter -
  HF908_RS03315 (HF908_03355) ispB 701291..702259 (-) 969 WP_193028424.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46502.81 Da        Isoelectric Point: 10.3693

>NTDB_id=436551 HF908_RS03295 WP_193027555.1 696442..697707(-) (pilC) [Ralstonia pseudosolanacearum strain RUN2279]
MATRAPTANRSAAASARKTAAKKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIV
PKDIAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLSVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMGISDFFVKW
WYIIIFAPVIGIILYARAFKRSENVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YYDATMEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPLIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=436551 HF908_RS03295 WP_193027555.1 696442..697707(-) (pilC) [Ralstonia pseudosolanacearum strain RUN2279]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCTGCATCGGCACGCAAGACCGCCGCCAAGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAGATCTTCAAGGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAATGCGGTGCTGCGCAAGCAAGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCCGCGCGCGGCAAGAAGATCGTA
CCCAAGGACATCGCCTACTTCACGCGCCAGCTGTCTACCATGCTGAAAGCGGGCGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGGCACGCCAACCCGAACTTCACCCAGCTGCTGACGGAAATCCGTGTCGACATCGAGTCTGGCAGCAGCA
TGGCGCAGGCCTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGATGCGGGTGAGCAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTATCCGATCTCGGTGCTGAGTGTGGCTTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCGGCGTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCAGCCCCGACGCTGGTGGTGATGGGCATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTCGCGCCCGTCATCGGCATCATTCTGTATGCGCGCGCCTTCAAGCGCTCGGAGAATGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGGA
CGCTGGCCACCATGTTCTCGGCCGGGACGCCGCTGGTGGAGTCGATGGAATCCGTGGCGGGCGCCGCCGGCAACTGGGTC
TACTATGACGCCACCATGGAGATCCAGCAGGCGGTGCGCATCGGTACCAGCCTCACCAACGCCATGCAGGCGACCCATGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGTGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AATTCTACGAGCGCGAAGTCGATGACGCGGTGGCCAACATCTCCGCCCTGATCGAGCCGCTCATCATCGTGATCCTGGGC
GTGCTGATCGGGGGCATGGTGGTTGCCATGTACCTGCCGATCTTCAAGCTGGGCCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

50.248

95.962

0.482
  pilG Neisseria gonorrhoeae MS11

50.627

94.774

0.48
  pilG Neisseria meningitidis 44/76-A

50.376

94.774

0.477
  pilC Acinetobacter baumannii D1279779

47.666

96.675

0.461
  pilC Acinetobacter baylyi ADP1

46.929

96.675

0.454
  pilC Legionella pneumophila strain ERS1305867

45.813

96.437

0.442
  pilC Vibrio cholerae strain A1552

39.75

95.012

0.378
  pilC Vibrio campbellii strain DS40M4

39.552

95.487

0.378