Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HF313_RS07765 Genome accession   NZ_CP051166
Coordinates   1778546..1780273 (-) Length   575 a.a.
NCBI ID   WP_126074536.1    Uniprot ID   A0A430HMD3
Organism   Massilia atriviolacea strain SOD     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1773546..1785273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF313_RS07740 (HF313_07750) zapD 1773611..1774366 (-) 756 WP_126074531.1 cell division protein ZapD -
  HF313_RS07745 (HF313_07755) coaE 1774446..1775078 (-) 633 WP_126074532.1 dephospho-CoA kinase -
  HF313_RS07750 (HF313_07760) pilD 1775080..1775970 (-) 891 WP_126074533.1 A24 family peptidase Machinery gene
  HF313_RS07755 (HF313_07765) - 1776157..1777248 (+) 1092 WP_126074534.1 acyltransferase -
  HF313_RS07760 (HF313_07770) pilG 1777314..1778534 (-) 1221 WP_126074535.1 type II secretion system F family protein Machinery gene
  HF313_RS07765 (HF313_07775) pilB 1778546..1780273 (-) 1728 WP_126074536.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HF313_RS07770 (HF313_07780) - 1780497..1781777 (-) 1281 WP_126074537.1 HlyC/CorC family transporter -
  HF313_RS07775 (HF313_07785) - 1781943..1782503 (-) 561 WP_126074538.1 ATP-dependent Clp protease proteolytic subunit -
  HF313_RS07780 (HF313_07790) uraH 1782571..1782924 (-) 354 WP_126074539.1 hydroxyisourate hydrolase -
  HF313_RS07785 (HF313_07795) puuE 1783046..1783990 (+) 945 WP_126074540.1 allantoinase PuuE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62567.31 Da        Isoelectric Point: 5.5840

>NTDB_id=436483 HF313_RS07765 WP_126074536.1 1778546..1780273(-) (pilB) [Massilia atriviolacea strain SOD]
MAAVLPNTTPGTPMPGLARALMQAGRLSAQQADSLHKKSLADKSPFIDTLLLTGTFDARTLAAFCAETFGYPLMDLQSFN
LDALPANAIDAKLMQSQRVIALAKRGNKMSVAISDPTNIQALDQIKFQSEASVEPVIVLHDVLLNLLSNLGRNAEQSIND
MVGDQEEIQFGEEDAPSTVIDQGAAEIEDAPIVKFLNKILMDAINLGASDIHFEPFEKFYRIRLRVDGMLKEHAQPPITI
RDKLVSRIKVLSKLDISEKRVPQDGRMRLILSPTKAIDFRVSTCPTLFGEKTVMRILDATQAQMGIDALGYDPDQKELLL
EAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNINDRSGLTFPVALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVIMITAQRLARRLCTCKKPVETSREIL
LAAGFREEELEGGWKPYGPVGCDRCSGGGYKGRVGIYQIMPITPAIEKIILAHGNSMEIAAQSEAEGVKSLRRSGLMKVK
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=436483 HF313_RS07765 WP_126074536.1 1778546..1780273(-) (pilB) [Massilia atriviolacea strain SOD]
ATGGCTGCAGTCCTCCCGAATACCACCCCCGGAACCCCGATGCCGGGCCTGGCCCGCGCCCTGATGCAAGCTGGCCGCCT
GAGTGCGCAGCAAGCCGATTCGCTGCATAAAAAATCGCTGGCGGACAAGAGCCCTTTCATCGACACCCTGCTGCTGACCG
GCACCTTCGACGCGCGCACCCTGGCCGCGTTTTGCGCGGAAACCTTCGGCTACCCGCTGATGGATTTGCAGAGCTTTAAC
CTGGATGCCTTGCCGGCCAACGCCATCGACGCCAAGCTGATGCAGAGCCAGCGCGTGATCGCCCTGGCCAAGCGCGGCAA
CAAGATGTCGGTCGCGATTTCCGACCCCACCAATATCCAGGCGCTCGACCAGATCAAGTTCCAGAGCGAAGCCTCGGTCG
AGCCGGTGATCGTGCTGCACGACGTGCTGCTGAACCTGCTGTCCAACCTGGGCCGCAACGCCGAGCAGAGCATCAACGAC
ATGGTGGGCGACCAGGAAGAAATCCAGTTCGGCGAGGAAGACGCGCCGTCCACGGTGATCGACCAGGGCGCGGCCGAGAT
CGAAGACGCGCCGATCGTCAAGTTCCTCAACAAGATCCTGATGGACGCGATCAACCTGGGCGCGTCCGATATCCACTTCG
AGCCGTTCGAAAAATTCTACCGCATCCGCCTGCGCGTGGACGGCATGCTCAAGGAGCACGCCCAGCCGCCGATCACCATC
CGCGACAAGCTGGTCTCGCGCATCAAGGTGCTCTCCAAGCTCGACATCTCCGAAAAGCGCGTGCCGCAGGATGGCCGCAT
GCGCCTGATCCTGTCGCCCACCAAGGCGATCGACTTCCGGGTCAGCACCTGCCCCACCCTGTTCGGCGAAAAGACCGTCA
TGCGTATCCTGGACGCGACCCAGGCCCAGATGGGCATCGACGCGCTCGGCTACGATCCGGACCAGAAGGAATTGCTGCTC
GAAGCGATCCAGCGCCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAGACGGTGTCGCTGTACACCTG
CCTGAACGTGATCAACAAACCGGGCATCAATATTTCGACCGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCGTGAACC
AGGTCAACATCAACGACCGCTCCGGCCTGACCTTCCCGGTCGCGCTCAAGTCCTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCGAC
CCTGCACACCAACGATGCCCCATCGACCCTGACGCGCCTGATGAACATGGGCGTGGCGCCCTTTAACATCGCTTCCTCGG
TGATCATGATCACCGCCCAGCGCCTGGCGCGCCGCCTGTGCACCTGCAAGAAGCCGGTCGAGACCAGCCGCGAAATCCTG
CTCGCGGCCGGCTTCCGGGAAGAGGAACTGGAAGGCGGCTGGAAGCCCTACGGCCCGGTCGGCTGCGACCGCTGCTCGGG
CGGCGGCTACAAGGGCCGGGTCGGTATCTACCAGATCATGCCGATTACCCCGGCGATCGAAAAAATCATCCTGGCGCACG
GCAATTCGATGGAAATTGCCGCGCAATCGGAAGCGGAGGGCGTCAAATCCCTGCGCCGCTCCGGCCTGATGAAAGTAAAA
CAAGGCTTGACCAGCCTTGAAGAAGTTCTCGGCTGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-155)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A430HMD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.276

99.652

0.541
  pilB Acinetobacter baylyi ADP1

54.021

99.478

0.537
  pilF Neisseria gonorrhoeae MS11

53.464

97.913

0.523
  pilB Legionella pneumophila strain ERS1305867

48.577

97.739

0.475
  pilB Vibrio cholerae strain A1552

46.004

97.913

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

97.739

0.443
  pilB Vibrio campbellii strain DS40M4

44.248

98.261

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.571

97.391

0.376
  pilF Thermus thermophilus HB27

39.626

93.043

0.369