Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IL983_RS19645 Genome accession   NZ_CP063047
Coordinates   4274625..4275089 (-) Length   154 a.a.
NCBI ID   WP_021205141.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain KC-Tt-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4269625..4280089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL983_RS19630 (IL983_19630) nadC 4272135..4272983 (+) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IL983_RS19640 (IL983_19640) - 4273164..4274543 (-) 1380 WP_232095709.1 O-antigen ligase family protein -
  IL983_RS19645 (IL983_19645) pilA/pilAI 4274625..4275089 (-) 465 WP_021205141.1 pilin Machinery gene
  IL983_RS19650 (IL983_19650) pilB 4275320..4277020 (+) 1701 WP_033956582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IL983_RS19655 (IL983_19655) pilC 4277024..4278241 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  IL983_RS19660 (IL983_19660) pilD 4278242..4279114 (+) 873 WP_034036018.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  IL983_RS19665 (IL983_19665) coaE 4279111..4279722 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  IL983_RS19670 (IL983_19670) yacG 4279719..4279919 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16076.44 Da        Isoelectric Point: 9.0145

>NTDB_id=436244 IL983_RS19645 WP_021205141.1 4274625..4275089(-) (pilA/pilAI) [Pseudomonas aeruginosa strain KC-Tt-1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPANWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=436244 IL983_RS19645 WP_021205141.1 4274625..4275089(-) (pilA/pilAI) [Pseudomonas aeruginosa strain KC-Tt-1]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTGCAGTTGGAAGCCAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGCTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.945

94.805

0.455
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA Acinetobacter baumannii strain A118

45.205

94.805

0.429
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37