Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KU884_RS14355 Genome accession   NZ_CP050695
Coordinates   3038205..3039929 (+) Length   574 a.a.
NCBI ID   WP_167783268.1    Uniprot ID   A0A6H0IXY0
Organism   Aquisalimonas sp. 2447     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3033205..3044929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU884_RS14335 (HC341_14270) - 3033207..3033701 (+) 495 WP_167783264.1 TRAP transporter small permease subunit -
  KU884_RS14340 (HC341_14275) - 3033705..3035030 (+) 1326 WP_167783265.1 TRAP transporter large permease subunit -
  KU884_RS14345 (HC341_14280) - 3035086..3035868 (+) 783 WP_167783266.1 3-hydroxybutyrate dehydrogenase -
  KU884_RS14350 (HC341_14285) - 3035931..3037910 (+) 1980 WP_167783267.1 acetoacetate--CoA ligase -
  KU884_RS14355 (HC341_14290) pilB 3038205..3039929 (+) 1725 WP_167783268.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KU884_RS14360 (HC341_14295) pilC 3039935..3041161 (+) 1227 WP_167783269.1 type II secretion system F family protein Machinery gene
  KU884_RS14365 (HC341_14300) pilD 3041180..3042052 (+) 873 WP_167783270.1 A24 family peptidase Machinery gene
  KU884_RS14370 (HC341_14305) coaE 3042128..3042766 (+) 639 WP_167783271.1 dephospho-CoA kinase -
  KU884_RS14375 (HC341_14310) zapD 3042766..3043557 (+) 792 WP_167783272.1 cell division protein ZapD -
  KU884_RS14380 (HC341_14315) - 3043568..3043780 (+) 213 WP_167783273.1 DNA gyrase inhibitor YacG -
  KU884_RS14385 (HC341_14320) - 3043899..3044828 (-) 930 WP_167783274.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62891.05 Da        Isoelectric Point: 5.3292

>NTDB_id=434045 KU884_RS14355 WP_167783268.1 3038205..3039929(+) (pilB) [Aquisalimonas sp. 2447]
MATANPTMKLSGLAGRLVRDGLIQEADARGAIEAARKAREPLVAHLVDKGLVSARDLAHAGAAEFGVPLMDLDAMEPDTA
VVKLVKEKLIRHHQVLPLVKRGKRLFVAVTDATNLEALDEFKFHTGLTTEPVLVEYDKLQRAIEKALDAADSNFGMGDDD
LDGDLENVQVTSGDDDDTVTDASEADADDAPVVRFVNKVLVDAINKGASDIHFEPYEKKYRVRFRQDGMLHTVASPPLGL
SPRISARLKVMSRMDIAERRVPQDGRIKMNLSKSRAIDFRVSSCPTLFGEKIVLRILDPNSAMLGIDKLGYEPEQKDRFM
NAIHKPYGMVLVTGPTGSGKTVSLYTALNILNTDDRNISTCEDPVEINLPGINQVNMNPKAGLTFAAALRAFLRQDPDIV
MVGEIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPSYNIASSVHLIIAQRLARRLCNNCKTPLDIPKET
LKDEGFTDEDLNDLKVFGPVGCDQCTQGYKGRVGIYQVMPVSEEMGRIIMEGGNAMQLEEQAAKEGINDLRRSGLMKVMQ
GVTSLDEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=434045 KU884_RS14355 WP_167783268.1 3038205..3039929(+) (pilB) [Aquisalimonas sp. 2447]
ATGGCGACCGCGAATCCCACCATGAAACTCAGTGGCCTGGCCGGACGACTGGTCCGCGACGGGCTCATCCAGGAAGCGGA
CGCGCGTGGTGCCATTGAGGCCGCCCGCAAGGCCCGCGAACCGCTGGTCGCCCACCTGGTGGACAAGGGGCTGGTCAGCG
CCCGTGACCTGGCGCACGCCGGCGCCGCGGAGTTCGGCGTACCGCTCATGGACCTGGACGCCATGGAGCCGGATACGGCG
GTGGTCAAGCTGGTCAAGGAAAAGCTGATCCGCCATCACCAGGTGCTGCCACTGGTCAAGCGAGGCAAGCGGCTGTTCGT
CGCCGTGACCGATGCCACCAACCTCGAGGCGCTGGACGAGTTCAAGTTCCACACCGGACTTACCACGGAACCGGTTCTGG
TGGAATACGACAAGCTGCAGCGCGCCATCGAGAAAGCGCTGGATGCTGCCGACAGCAACTTCGGCATGGGCGACGACGAT
CTGGACGGGGACCTGGAGAACGTTCAGGTCACCAGCGGTGACGACGACGATACCGTCACCGACGCCTCCGAGGCGGATGC
AGACGACGCACCGGTGGTCCGTTTCGTGAACAAGGTCCTGGTGGACGCCATCAACAAGGGCGCCTCGGACATCCACTTCG
AGCCCTACGAGAAAAAGTACCGGGTCCGCTTCCGCCAGGACGGCATGCTGCATACCGTGGCCAGCCCCCCGTTAGGCCTG
TCGCCGCGCATCTCGGCGCGGCTCAAGGTGATGTCGCGGATGGACATCGCAGAGCGACGGGTGCCCCAGGACGGCCGCAT
CAAGATGAACCTGTCCAAGAGCCGTGCCATCGACTTCCGCGTCAGCAGCTGCCCGACCCTGTTCGGCGAGAAGATCGTTC
TGCGTATCCTCGACCCGAACAGCGCCATGCTCGGTATCGACAAGCTCGGCTATGAACCCGAGCAGAAGGATCGCTTCATG
AACGCGATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACGGGCAGCGGCAAGACCGTGTCCCTGTACACGGC
GCTGAACATCCTCAACACCGACGACCGCAACATCTCCACCTGTGAGGACCCGGTGGAAATCAACCTGCCGGGCATCAACC
AGGTGAACATGAACCCCAAGGCGGGGCTCACTTTCGCCGCCGCCCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCGTC
ATGGTGGGTGAGATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCGGCTCAGACCGGCCACTTGGTGCTGTCCAC
GCTGCATACCAACGATGCCCCACAGACCCTCACGCGTCTGATGAACATGGGCGTGCCGTCGTACAACATTGCCTCGTCGG
TGCACCTGATCATCGCCCAGCGCCTTGCCCGGCGCCTATGCAACAACTGCAAGACACCCCTGGACATCCCCAAGGAAACG
CTGAAGGACGAGGGCTTCACCGACGAGGACCTGAACGACCTCAAGGTGTTCGGTCCGGTGGGTTGCGACCAGTGCACCCA
GGGCTACAAGGGCCGCGTGGGCATTTACCAGGTCATGCCGGTGAGCGAGGAGATGGGCCGCATCATCATGGAAGGCGGCA
ATGCCATGCAGCTGGAGGAACAGGCCGCCAAGGAGGGCATCAATGACCTGCGTCGCTCGGGACTCATGAAAGTCATGCAG
GGCGTCACCAGCCTGGATGAGATCAACCGCGTCACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H0IXY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.507

100

0.587
  pilB Acinetobacter baylyi ADP1

58.275

98.955

0.577
  pilB Legionella pneumophila strain ERS1305867

55.382

100

0.556
  pilF Neisseria gonorrhoeae MS11

49.735

98.78

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

48.932

97.909

0.479
  pilB Vibrio cholerae strain A1552

51.992

91.812

0.477
  pilB Vibrio campbellii strain DS40M4

48.142

98.432

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.338

92.857

0.375
  pilF Thermus thermophilus HB27

38.53

97.213

0.375